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Anti-ammonium engineering strain of Brasil diazotrophic spirillum containing multiple copied glnB gene DraT*

An Azospirillum brasiliensis, multi-copy technology, applied in the field of Azospirillum brasiliensis engineering strains, can solve the problem of NifA protein without transcriptional activation activity, and achieve the effect of reducing the amount of use

Inactive Publication Date: 2006-05-03
CHINA AGRI UNIV
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AI Technical Summary

Problems solved by technology

The nifA gene in Azospirillum brasiliensis can be expressed constitutively, but the expressed NifA protein has no transcriptional activation activity and needs the P protein encoded by the glnB gene. II Only under the action of the protein can it have transcriptional activation activity

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0015] The invention provides an engineering strain of Azospirillum brasiliensis, and the strain number is YuMB. The bacterial strain has been preserved by the General Microorganism Center of China Microorganism Culture Collection Management Committee on January 22, 2003, and the preservation number is: CGMCC No.0875.

[0016] The present invention will be further described through the construction example of the engineering strain YuMB below.

[0017] 1. Construction of engineering strain YuMB of Azospirillum brasiliensis

[0018] The construction of Azospirillum brasiliensis engineering strain YuMB mainly includes the following three steps:

[0019] (1) Azospirillum brasiliensis DraT - Mutant strain construction

[0020]In the present invention, Azospirillum brasiliensis Yu62 is inoculated into LD liquid medium (10 g of tryptone, 2.5 g of NaCl, 5 g of yeast powder, 1000 ml of distilled water, pH6.8), placed on a constant temperature shaker at 200 rpm, and cultured at 30° ...

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Abstract

An Azospirillum brasilense DraT- anti-ammonium engineering strain containing multi-copied glnB gene is disclosed, with preserving number of CGMCC No.08765. This bacterial manure, containing anti-ammonium engineering strain of Azospirillum brasilense DraT- with multi-copied glnB is also disclosed. The cultured engineering strain as bacterial manure is applied for field plant of corn and wheat to decrease use amount of urea, increase yield and save manure, promote plant growth by IAA.

Description

technical field [0001] The invention relates to an engineering bacterial strain of Azospirillum brasiliensis. The invention also relates to a bacterial fertilizer. Background technique [0002] The improvement of the output of most crops such as wheat, corn and rice mainly depends on the increase of the application of chemical nitrogen fertilizers. The unrestrained application of large amounts of chemical fertilizers with the sole goal of increasing the output will result in soil compaction, decreased fertility, and the environment especially Serious water pollution and other issues. [0003] Biological nitrogen fixation is an important way to reduce the use of nitrogen fertilizers, alleviate environmental pollution, and reduce agricultural production costs. According to the relationship between nitrogen-fixing microorganisms and host plants, nitrogen fixation can be divided into autogenous nitrogen fixation, joint nitrogen fixation and symbiotic nitrogen fixation (Edited ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C05F11/08
Inventor 陈三凤刘元李季伦
Owner CHINA AGRI UNIV
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