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CZ-81 strain of 'Baibachi' bacterium and artificial culture method

A technology of artificial cultivation of white rake tooth fungus, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of weak metabolic function, poor stability, and no cultivation methods, etc., to increase the content, The effect of strong stability and scientific control of production links

Inactive Publication Date: 2006-10-11
TONGHUA JITONG PHARMA
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Problems solved by technology

[0003] The fermented extracts isolated and purified from the strains of white rake tooth bacteria have good clinical effects on the treatment of chronic glomerulonephritis. The existing strains have poor stability and weak metabolic function. How to artificially cultivate high-quality white rake Dental bacteria strains, has not been a good culture method

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  • CZ-81 strain of 'Baibachi' bacterium and artificial culture method

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Embodiment

[0069] A strain with stable performance obtained through isolation, purification, screening, and systematic selection and evaluation from the dead standing wood of broad-leaved trees or the fruiting bodies of dead wood in the mixed coniferous and broad-leaved forest belt of Changbai Mountains in China. The method is:

[0070] (1) Level 1 slant strains:

[0071] Medium: Comprehensive PDA Medium: Glucose 2%, Potato 20%, Agar 2%, KH 2 PO 4 0.3%, MgSO 4 0.2%, VB10.05%, PH6.5 (before elimination).

[0072] Set up special personnel, special boxes (refrigerators), and special record management. Check and record the strain performance and aging condition at any time. Do a good job of marking and recording the subculture of the transfer tube, and the generation (number of passages) of the strain used in production must be clear. Abnormal and aging strains should be eliminated in time and should not be used in production.

[0073] The culture temperature of the slant strain is 26...

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Abstract

The invention relates to a culture method of a fungus bioengineering submerged fermentation cultured high-performance strain, that is, the culture method of the bacteria strain "CZ-81". CZ-81 strain CGMCC No. 1016. The artificial cultivation method includes strain cultivation, that is, strain cultivation on an inclined plane; strain cultivation in shake flasks; strain cultivation in seed tanks; fermentation production cultivation and extraction processing after putting into tanks. The strain CZ-81 of the white rake tooth bacteria is a strain with strong stability, high metabolic ability and high safety after years of selection and breeding. This strain is not easy to degenerate after 20 generations, and its metabolic index is more than 1 times higher than that of the original screened strain. The entire fermentation and cultivation time is shortened by nearly 20%. The method not only improves the content of effective active ingredients, but also shortens the fermentation and cultivation time, and reduces the production cost.

Description

Technical field: [0001] The invention relates to a method for cultivating high-performance strains cultured by submerged fermentation of fungal bioengineering, that is, the bacterial strain "CZ-81" of the fungi. Background technique: [0002] Irpex lacteus Fr. is a kind of fungus belonging to the family Irpex lacteus, the genus Irpex lacteus, and the species Irpex lacteus, also known as white sac hole. Synonyms: Hirschioporus lacteus (Fr.) Teng; Deng Shuqun, Fungi in China, 484.1963. Its fruiting bodies are born on dead standing or fallen wood of broad-leaved trees. Morphological characteristics: Basidiocarp (fruiting body) flattened, expanded, white, soft leathery; or flattened and rolled up, the rolled part is 8-15×10-20mm. Sometimes anatropous, stacked in the shape of compound tiles, fan-shaped, with a narrow base, 2-4cm in transverse diameter, and 2-3mm in original; cover surface is milky white, with short hairs, silky luster, ring pattern, thin edge, Recurved, wavy t...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 宋启印
Owner TONGHUA JITONG PHARMA