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Benzoquinone compound preparation method and application thereof

A technology of compounds and fermented products, which is applied in the field of preparation of cell proliferation inhibitors or antitumor agents, and can solve problems such as drugs that have not yet been seen

Inactive Publication Date: 2006-10-18
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inventor found that the crude extract of Penicillium terrestre LM2-02 (Penicillium terrestre) (preservation number: CCTCC M 204077) liquid fermentation product after ultrasonic crushing has good cell cycle inhibitory activity, and then its active ingredients As a result, two new benzoquinone compounds have been found to have antitumor activity, but there is no report on the chemical structure and cell proliferation inhibitory activity of the compound, so there is no related drug on the market.

Method used

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  • Benzoquinone compound preparation method and application thereof
  • Benzoquinone compound preparation method and application thereof
  • Benzoquinone compound preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1 Fermentation production and separation and purification of compound I-II

[0024] 1 Fermentation production

[0025] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take an appropriate amount of Penicillium terrestre LM2-02 (Penicillium terrestre) (preservation number: CCTCC M 204077), inoculate it on the PDA slant medium, and cultivate it in an incubator at 28 degrees Celsius 4 days.

[0026] Get an appropriate amount of Penicillium LM2-02 (Penicillium terrestre) cultured on the slant for 4 days, and inoculate it into 120mL culture medium [medium composition (g / L): maltose 20.0, mannitol 20.0, monosodium glutamate 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, yeast extract 3.0, pH natural] in the 500mL Erlenmeyer flask, at 28 ℃, 120 rev / min conditions shaker culture 48 hours, obtain the seed culture solution of Penicillium terrestre LM2-02 (Penicillium terrestre). This seed culture solution is respective...

Embodiment 2

[0033] The test of embodiment 2 antitumor activity

[0034] 1 Experimental samples and experimental methods

[0035] Preparation of the test sample solution: the test sample is the pure compound I-II isolated and refined in the above-mentioned Example 1. Accurately weigh an appropriate amount of sample and prepare a solution with the required concentration with DMSO for activity testing.

[0036] Cell lines and subculture of cells: P388 and A-549 cell lines were used for activity testing. All kinds of cells were subcultured in RPMI-1640 medium containing 10% FBS in an incubator with 5% carbon dioxide at 37°C.

[0037] Cell Proliferation Inhibitory Activity Test Method (MTT Method)

[0038]The invention adopts the MTT method to test and evaluate the inhibitory activity of the tested sample on the proliferation of cancer cells. The dehydrogenase in the mitochondria of living cells can metabolize and reduce the yellow 3-(4,5-dimethylthiazole)-2,5-diphenyltetrazolium bromide t...

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Abstract

The present invention relates to two kinds of benzoquinone compounds and their preparation method and application. The invention uses Penicillium terrestre LM2-02 (Penicillium terrestre) isolated from marine samples to produce benzoquinone compounds with novel structures. Experiments have proved that the compound can be used as a cell proliferation inhibitor or an antitumor agent.

Description

Technical field: [0001] The invention relates to a method for preparing benzoquinone compounds by using Penicillium terrestre LM2-02 (Penicillium terrestre) (preservation number: CCTCC M 204077); the invention also relates to the use of such compounds in the preparation of cell proliferation inhibitors or antitumor agents use. Background technique: [0002] Related benzoquinone compounds have a wide range of biological activities, mainly used as free radical scavengers, bactericidal and cytotoxic activities. Such as Suzuki Y, Kono Y, Inoue T, Sakurai A.A potent antifugal benzoquinone in etiolatedsorghum seedlings and its metabolites. Phytochemistry, 1998, 47(6):997-1001; Kubo, I, Kamikawa T, Miura I. Isolation, structure and synthesis of maesanin, a host defense stimulus from an African medicinal plant Maesa lanceolata. Tetrahedron Lett, 1983, 24, 3825-3828; Muhammad I, Takamatsu S, Walker L A, Mossa J S, Fong H H S, El-Feraly F S. Cytotoxic and antioxidant activities of A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C50/38C07C46/00A61K31/122A61P35/00
Inventor 朱伟明刘为忠顾谦群朱天骄刘红兵方玉春
Owner OCEAN UNIV OF CHINA
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