Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for making bio-chip

A technology of biochips and derivatives, applied in biochemical equipment and methods, measurement/inspection of microorganisms, biological testing, etc., can solve problems such as the difficulty of achieving accurate quantification, and achieve easy industrial production, uniform shape and size, and good repeatability Effect

Inactive Publication Date: 2006-10-25
INST OF MECHANICS - CHINESE ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result of this spotting method, the current biochip can only be used for qualitative or semi-quantitative measurements, and it is very difficult to achieve accurate quantification

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for making bio-chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] refer to figure 1 , a method for preparing a methyl polyethylene glycol derivative biochip with a silicon wafer as a substrate in detail

[0021] 1. First make the silicone stamp: apply the photoresist on the silicon wafer, and make a mold with 500 circular patterns through conventional photolithography technology;

[0022] 2. Pour commercially available PDMS silica gel on the mold made in step 1, and after the PDMS silica gel is solidified, remove it from the mold to obtain a silica gel stamp with a circular pattern and a diameter of 20 microns;

[0023] 3. Put the polyethylene glycol derivative Cl on the silicone stamp made in step 2 2 Si(CH 2 ) 11 (OCH 2 CH 2 ) 3 OCH 3 The solution is coated on the silicone stamp made in step 2, and transferred on the silicon wafer;

[0024] 4. Soak the silicone stamp made in step 3 in polyethylene glycol derivative Cl 3 Si(CH 2 ) 11 (OCH 2 CH 2 ) 3 OCH 2 COOCH 2 CH 3 Medium modification without polyethylene glycol d...

Embodiment 2

[0026] refer to figure 1 , a method for preparing a hydroxypolyethylene glycol derivative biochip with a gold-plated silicon wafer as a substrate in detail

[0027] 1. First make a silicone stamp: apply the photoresist on the silicon wafer, and make a pattern with 10,000 squares through conventional photolithography technology;

[0028] 2. Pour commercially available PDMS silica gel on the mold made in step 1, and after the PDMS silica gel solidifies, remove it from the mold to obtain a silica gel stamp with a square pattern and a side length of 100 microns;

[0029] 3. Place the polyethylene glycol derivative HS (CH) on the silicone stamp made in step 2 2 ) 11(OCH 2 CH 2 ) 3 The OH solution is coated on the silicone stamp made in step 2, and transferred to the silicon wafer;

[0030] 4. Soak the silicone stamp made in step 3 in polyethylene glycol derivative HS (CH 2 ) 11 (OCH 2 CH 2 ) 7 OCH 2 Modified non-polyethylene glycol derivatives HS(CH 2 ) 11 (OCH 2 CH ...

Embodiment 3

[0032] refer to figure 1 , a method for preparing a methyl polyethylene glycol derivative biochip with a glass sheet as a substrate in detail

[0033] 1. First make the silicone stamp: apply the photoresist on the silicon wafer, and make a mold with 1500 circular patterns through conventional photolithography technology;

[0034] 2. Pour commercially available PDMS silica gel on the mold made in step 1, and after the PDMS silica gel is solidified, remove it from the mold to obtain a silica gel stamp with a circular pattern and a diameter of 200 microns;

[0035] 3. Put the polyethylene glycol derivative (CH 3 CH 2 O) 3 Si(CH 2 ) 11 (OCH 2 CH 2 ) 3 OCH 3 The solution is coated on the silicone stamp made in step 2, and transferred on the silicon wafer;

[0036] 4. Soak the silicone stamp made in step 3 in polyethylene glycol derivatives (CH 3 CH 2 O) 3 Si(CH 2 ) 11 (OCH 2 CH 2 ) 3 Modified non-polyethylene glycol derivatives in OCHO (CH 3 CH 2 O) 3 Si(CH 2 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

A biochip manufacturing method of the present invention comprises the following steps: firstly making a silica gel seal; then coating the polyethylene glycol derivative A used to format the solid surface on the surface of the seal made in step 1, and then covering it on a spare part On the solid surface, transfer the polyethylene glycol derivative A on the solid surface according to the designed pattern; finally, immerse the formatted solid substrate in the polyethylene glycol derivative B solution. The alcohol derivative B is bound in the blank area not covered by the polyethylene glycol derivative A; the active groups on the polyethylene glycol derivative B are activated by conventional chemical methods, and the biological sample is spotted by a mechanical spotter. The area covered by polyethylene glycol derivative B on the solid surface; after incubation; and after the biomolecules that are not covalently fixed on the surface are washed away with water, a biomolecular film layer with uniform distribution is obtained. The method is simple to operate and has good repeatability; and the prepared biochip dots are uniform in shape and size, and biomolecules in each dot are evenly distributed.

Description

technical field [0001] The invention relates to a method for making a biochip, in particular to a method for making a biochip in which sample points made on the surface of the biochip are uniform in shape and size, and biological samples in a single point are evenly distributed. Background technique [0002] Biochips have become a booming field in biotechnology. In biological research, disease diagnosis, environmental monitoring, etc., biochips have great potential application prospects. There are many ways to make biochips. At present, the most widely used and simplest method is the mechanical pointing method, in which biological samples are added to the substrate with a sampler, and arranged on the substrate in the form of a matrix. Spotting methods can be divided into two categories, contact and non-contact spotting. Although the mechanical spotting method can accurately control the amount of biological samples at each point, the spots on the substrate cannot be unifor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50G01N33/68C12Q1/68
Inventor 王战会靳刚
Owner INST OF MECHANICS - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com