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Method for making bio-chip

A technology of biochips and production methods, applied in the directions of biochemical equipment and methods, measurement/inspection of microorganisms, biological testing, etc., can solve the problems of difficulty in realizing accurate quantification, etc., and achieve easy industrial production, uniform shape and size, and good repeatability Effect

Inactive Publication Date: 2005-03-23
INST OF MECHANICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result of this spotting method, the current biochip can only be used for qualitative or semi-quantitative measurements, and it is very difficult to achieve accurate quantification

Method used

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  • Method for making bio-chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] refer to figure 1 , a method for preparing a methyl polyethylene glycol derivative biochip with a silicon wafer as a substrate in detail

[0021] 1. First make the silicone stamp: apply the photoresist on the silicon wafer, and make a mold with 500 circular patterns through conventional photolithography technology;

[0022] 2. Pour commercially available PDMS silica gel onto the mold made in step 1, and after the PDMS silica gel is solidified, remove it from the mold to obtain a silica gel stamp with a circular pattern and a diameter of 20 microns;

[0023] 3. Put the polyethylene glycol derivative Cl on the silicone stamp made in step 2 3 Si(CH 2 ) 11 (OCH 2 CH 2 ) 3 OCH 3 The solution is coated on the silicone stamp made in step 2, and transferred on the silicon wafer;

[0024] 4. Soak the silicone stamp made in step 3 in polyethylene glycol derivative Cl 3 Si(CH 2 ) 11 (OCH 2 CH 2 ) 3 OCH 2 COOCH 2 CH 3 Medium modification without polyethylene glyc...

Embodiment 2

[0026] refer to figure 1 , a method for preparing a hydroxypolyethylene glycol derivative biochip with a gold-plated silicon wafer as a substrate in detail

[0027] 1. First make a silicone stamp: apply the photoresist on the silicon wafer, and make a pattern with 10,000 squares through conventional photolithography technology;

[0028] 2. Pour commercially available PDMS silica gel on the mold made in step 1, and after the PDMS silica gel solidifies, remove it from the mold to obtain a silica gel stamp with a square pattern and a side length of 100 microns;

[0029] 3. Place the polyethylene glycol derivative HS (CH) on the silicone stamp made in step 2 2 )11 (OCH 2 CH 2 ) 3 The OH solution is coated on the silicone stamp made in step 2, and transferred to the silicon wafer;

[0030] 4. Soak the silicone stamp made in step 3 in polyethylene glycol derivative HS (CH 2 ) 11 (OCH 2 CH 2 ) 7 OCH 2 Modified non-polyethylene glycol derivatives HS(CH 2 ) 11 (OCH 2 CH ...

Embodiment 3

[0032] refer to figure 1 , a method for preparing a methyl polyethylene glycol derivative biochip with a glass sheet as a substrate in detail

[0033] 1. First make the silicone stamp: apply the photoresist on the silicon wafer, and make a mold with 1500 circular patterns through conventional photolithography technology;

[0034] 2. Pour commercially available PDMS silica gel on the mold made in step 1, and after the PDMS silica gel is solidified, remove it from the mold to obtain a silica gel stamp with a circular pattern and a diameter of 200 microns;

[0035] 3. Put the polyethylene glycol derivative (CH 3 CH 2 O) 3 Si(CH 2 ) 11 (OCH 2 CH 2 ) 3 OCH 3 The solution is coated on the silicone stamp made in step 2, and transferred on the silicon wafer;

[0036] 4. Soak the silicone stamp made in step 3 in polyethylene glycol derivatives (CH 3 CH 2 O) 3 Si(CH 2 ) 11 (OCH 2 CH 2 ) 3 Modified non-polyethylene glycol derivatives in OCHO (CH 3 CH 2 O) 3 Si(CH 2 ...

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Abstract

The invention discloses a biology chip producing method, it includes following steps: at first, produce silica gel print, then smear polyethylene glycol derivative A which is used to format solid surface on the print surface, then put it on the solid surface; at last, dip the formatted solid chip in the polyethylene glycol derivative B solution, the B combine in the blank field which is not covered by the A; act the active radical of the B by normal method, put the biology sample on the surface which is not covered by the B by mechanical spotting machine; after hatching, rinse the rest biology molecule which is not fixed on the surface, then a distributed equably molecule film can be achieved. The method is operated easily, and has good repeatability; and the produced chip dots have accordant shape and size, and within each dot the molecules is distributed equably.

Description

technical field [0001] The invention relates to a method for making a biochip, in particular to a method for making a biochip in which sample points made on the surface of the biochip are uniform in shape and size, and biological samples in a single point are evenly distributed. Background technique [0002] Biochips have become a booming field in biotechnology. In biological research, disease diagnosis, environmental monitoring, etc., biochips have great potential application prospects. There are many ways to make biochips. At present, the most widely used and simplest method is the mechanical pointing method, in which biological samples are added to the substrate with a sampler, and arranged on the substrate in the form of a matrix. Spotting methods can be divided into two categories, contact and non-contact spotting. Although the mechanical spotting method can accurately control the amount of biological samples at each point, the spots on the substrate cannot be unifor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/50G01N33/68
Inventor 王战会靳刚
Owner INST OF MECHANICS - CHINESE ACAD OF SCI
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