Hybridizing method for obtaining allotranssituation system for anti downy mildew of cucumbus
A technology for resisting downy mildew and translocation lines, applied in the fields of botanical equipment and methods, application, plant gene improvement, etc., can solve the problem of insufficient resistance to cucumber downy mildew, narrow genetic basis of cucumber, and difficulty in disease-resistant genes, etc. problem, to achieve the effect of rich morphological types, strong resistance, and comprehensive identification technology
Inactive Publication Date: 2006-11-08
NANJING AGRICULTURAL UNIVERSITY
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[0006] Technical Problems The purpose of this invention is to provide a set of cucumber downy mildew resistant heterologous translocation lines in view of the current situation that it is difficult to directly use exogenous disease resistance genes of the genus Melon, the genetic basis of cucumber is narrow, and the source of resistance to cucumber downy mildew is insufficient. Breeding method, obtaining disease-resistant heterologous translocation lines of Melon, providing new materials for cucumber disease-resistant breeding, and cultivating new disease-resistant cucumber varieties
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Embodiment 1
[0022] Example 1 The breeding method of cucumber downy mildew resistant heterologous translocation line CT-01, including:
[0023] 1) Using artificially synthesized new amphidiploid species (Patent No. ZL 00 1 12271.1, Publication No.: CN 1279009A, Publication Date: January 10, 2001) as the male parent, common cucumber "Beijing Truncated" (publicly known Public varieties, preserved in the National Vegetable Germplasm Resources Medium-term Bank of the Chinese Academy of Agricultural Sciences) as the female parent, backcrossed, in the backcrossed population, the root tip meristematic cell chromosome preparation technology and field inoculation test for resistance to downy mildew were directly screened Obtain the number of chromosomes 2n=14( Figure 4 .A), resistant to downy mildew, the disease level is 1.8 ± 0.5 (Table 1) single plant HH 1-8 ;
[0024] 2) For single plant HH 1-8 Carry out self-breeding, and sow in the second year to continue field inoculation identification o...
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The breeding method of the cucumber downy mildew-resistant heterologous translocation line CT-01 of the invention is specially used for cucumber disease-resistant breeding, and uses the new amphidiploid species of the genus Melon to backcross with the cultivated cucumber parent "Beijing Truncated". Through root tip meristematic cell chromosome preparation technology and downy mildew resistance field inoculation test, the downy mildew resistant single plant with chromosome number 2n=14 was directly screened from the backcross progeny, and the self-bred progeny was tracked and identified, and the obtained A heterologous translocation line CT-01 of cucumber with high resistance to downy mildew.
Description
1. Technical field [0001] The invention relates to a breeding method of the downy mildew resistant heterologous translocation line CT-01 of cucumber, belonging to the field of biotechnology breeding. 2. Background technology [0002] For more than ten years, the parental resources of cucumber hybrid varieties cultivated in China are limited, and there is even a phenomenon that one parent is reused in multiple varieties. Therefore, in the current cucumber breeding, the lack of specific and excellent germplasm resources is an urgent problem to be solved, and the innovation of specific germplasm resources such as disease resistance, insect resistance and stress resistance of cucumber can provide an effective way way. However, due to the existence of genetic or physiological barriers between distant species, it is difficult to successfully hybridize. [0003] In the patent "Method of Cucumber Breeding for Wild Melon Species" (Patent No. ZL 00 1 12271.1, Publication No.: CN 127...
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IPC IPC(8): A01H1/02
Inventor 陈劲枫曹清河钱春桃娄群峰
Owner NANJING AGRICULTURAL UNIVERSITY