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Method and appts. for selective biological material detection

A bioassay and biological activity technology, applied in the field of detection of pathogenic microorganisms or biological substances, can solve problems such as unexpected sensors

Inactive Publication Date: 2002-01-16
TOXIN ALERT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The Berkeley researchers did not envisage putting sensors into food packaging, nor did they envision the variety of sensors capable of detecting and identifying pathogenic contamination sources in the hands of untrained end users such as food buyers or consumers.

Method used

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  • Method and appts. for selective biological material detection
  • Method and appts. for selective biological material detection
  • Method and appts. for selective biological material detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Antibody Detection on the Surface of Pretreated Thin Layer Polyethylene Foils:

[0057] 0.1M Sodium Carbonate (Na 2 CO 3 )-sodium bicarbonate (NaHCO 3 ) buffer to dilute rabbit polyclonal IgG to a final concentration of 2.0 μg / ml.

[0058] Using a 2"x3" grid, 75 μL (150 ng) was applied to the pretreated polyethylene sheet with a 1" gap.

[0059] The antibody-treated polyethylene sheets were dried at 37°C for 1.5 hours.

[0060] The dried film was washed 3 times with pH 7.4 phosphate buffer.

[0061] In 1% casein, 0.1M potassium ferricyanide K 3 Fe(CN) 6 , 0.1% phosphate glass (Na 15 P 13 o 40 -Na 20 P 18 o 55 ) diluted goat anti-rabbit IgG conjugated to HRP (GαR HRP ) to a concentration of 1:7000, pH7.4.

[0062] Add 125 μL of diluted G with a precision pipette HRP into the grid-backed polyethylene film at 1" intervals, coinciding with where previously covered by RαG.

[0063] The flakes were incubated for 30 minutes at room temperature.

[00...

Embodiment 2

[0068] Example 2 Whole Sandwich Immunoassay on the Surface of Pretreated Thin Layer Polyethylene Foils

[0069] 0.1M Sodium Carbonate (Na 2 CO 3 )-sodium bicarbonate (NaHCO 3 ) buffer to dilute rabbit polyclonal IgG to a final concentration of 2.0 μg / ml.

[0070] A 13 x 9 cm pretreated thin layer polyethylene sheet from DuPont was inserted into a BIO-RAD DOT-SPOT device with 96 sample wells in a 12 x 8 well grid spaced at 1.0 cm intervals.

[0071] 100 μL of rabbit polyclonal IgG sample (1.0 μg) was added to each of the 8 wells in column 1.

[0072] In columns 2-12 are serial dilutions of antibody from 500ng-0.5ng, respectively.

[0073] Antibody-treated polyethylene sheets were dried overnight at 37°C.

[0074] The dried film was washed 3 times with pH 7.4 phosphate buffered saline (PBS).

[0075] Antigen was diluted to a final concentration of 1.0 μg / ml in tris buffer (TBS) containing 1% casein at pH 7.4.

[0076] 100 μL of antigen (representing 100 ng) was applied to...

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Abstract

The present invention relates to bioassay materials useful for the detection of toxic substances and, more particularly, to packaging materials for food and other products, along with methods for their manufacture and use. The invention provides a unique composite material capable of detecting and identifying multiple biological materials within a single package. The biological material identification system is designed for incorporation into existing types of flexible packaging material such as polyolefin films, and its introduction into the existing packaging infrastructure will require little or no change to present systems or procedures.

Description

field of invention [0001] The present invention relates to the detection of pathogenic microorganisms or biological substances, more specifically, a combined bioassay material for the detection of particularly toxic substances, its manufacturing method and application method, where the composite material is particularly useful for food packaging and the like Useful and capable of simultaneous detection and identification of such a diversity of biological substances. Background of the invention [0002] Despite considerable effort and expense to control the growth of pathogenic microorganisms from food, significant safety concerns remain in the packaged food supply. For example, contamination of food ingredients with strains of Escherichia coli, Campylobacter, Listeria, Cyclospora, and Salmonella has caused countless food poisoning incidents, resulting in illness and even death, not to mention the risk to food manufacturers. A lot of income that came was lost. These and oth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/545G01N33/558G01N33/569
CPCG01N33/545G01N33/54393G01N33/558G01N33/54386G01N33/569Y10S436/805G01N33/54391
Inventor 威廉·T·博登哈默
Owner TOXIN ALERT