Immunity colloidal gold reagent for detecting IgG antibody of giant cells virus and its preparing method

A cytomegalovirus and colloidal gold technology, applied in the field of preparation of the reagent, can solve the problems of inability to realize single-unit detection, inability to realize one-step operation, long detection time, etc., and achieve easy popularization and use, fast detection speed, low cost effect

Inactive Publication Date: 2003-07-23
博顿生物检验技术(杭州)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection High, unable to achieve single-person detection
The disadvantages of the latex agglutination method ar

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] Adhere one end of the PVC backing to the sample pad, the binding pad in turn, the nitrocellulose membrane in the middle, and the other end to the absorbent pad. The binding pad is coated with protein A (or rabbit anti-human IgG)-colloidal gold marker. The nitrocellulose membrane is coated with cytomegalovirus-specific surface membrane antigen and chicken anti-protein A (or goat anti-rabbit IgG), depending on whether protein A or rabbit anti-human IgG is used for labeling. If the colloidal gold is labeled with protein A, use chicken anti-protein A as the control line; if the colloidal gold is labeled with rabbit anti-human IgG, use goat anti-rabbit IgG as the control line.

[0008] Preparation according to the following steps: (1) Preparation of rabbit anti-human IgG: extract human antiserum to immunize rabbits, and obtain rabbit anti-human IgG after purification; (2) Preparation of polyclonal antibody: use cytomegalovirus-specific surface membrane antigen several times ...

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Abstract

The reagent includes the sample pad, the binding pad, the cellulose nitrate film, the water uptake pad and the PVC back cover. The sample pad, the binding pad are adhered to the one end with PVC backcovered in sequence. The cellulose nitrate film is adhered to the middle. The water uptake pad is adhered to another end of the reagent. The binding pad is coated with the label of albumen A (or rabbit anti human IgG)-colloidal gold. The cellulose nitrate film is coated with the specificity surface film antigen of giant cells virus and chickens anti albumen A (or sheep anti rabbit IgG). The reagent features high specificity and sensitivity, simple and fast. The reagent is applicable to the detection in site within 30 minutes for the full testing procedure.

Description

technical field [0001] The invention relates to an immune colloidal gold reagent for detecting antibodies, and also relates to a preparation method of the reagent. Background technique [0002] Existing methods for detecting CMV antibody IgG mainly include enzyme-linked immunosorbent assay, latex agglutination, immunocolloidal gold diafiltration and so on. The disadvantages of the enzyme-linked immunosorbent (EIA) method are: special instruments and equipment such as microplate readers are required for use; detection operators need to undergo professional training; the operation process is relatively complicated, and the time required for detection is relatively long; the cost of detection Higher, single-person detection cannot be realized. The disadvantages of the latex agglutination method are: the operation process is relatively complicated and demanding, and one-step operation cannot be realized; the reagent needs to be stored at low temperature; ...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/531G01N33/543G01N33/569
Inventor 张少恩
Owner 博顿生物检验技术(杭州)有限公司
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