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Experimental buffer solution and its preparation method

A buffer and experimental technology, applied in material inspection products, instruments, biological testing, etc., can solve the problems of inaccurate weighing of raw materials, inconvenient storage, cumbersome and other problems, and achieve the effect of small size and easy portability.

Inactive Publication Date: 2004-03-03
长沙福滋堂生物技术开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently used buffer solution for experiments is mainly for temporary preparation or purchase of liquid preparations by laboratory personnel, and in the process of temporary preparation, the weighing of raw materials is inaccurate and cumbersome, and there are often differences in weighing from person to person or some errors, resulting in inaccurate experimental buffers
However, a small amount of commercially available liquid agents require containers such as glass bottles, which take up a lot of space, are inconvenient for transportation and storage, and the liquid agents are not very stable.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Preparation of PBS phosphate buffer solid dosage form. Use the same raw material ratio as PBS phosphate buffer solution, NaCl:kCl:Na 2 HPO 4 .7H 2 o: KH 2 PO 4 =80:2:11.5:2 by weight. Accurately weigh the above-mentioned raw material particles with a precision balance according to the above-mentioned ratio, grind and pulverize them, and sieve them to 20 meshes, add microcrystalline fiber accounting for 1 / 10 of the total weight of the raw materials, and paraffin wax accounting for 1 / 10 of the total weight of the raw materials. The above raw materials are mixed, placed in a tablet press and pressed into reagent discs with a diameter of 0.5 cm or 1 cm or 2 cm or 5 cm. Finally, let it dry. The pressed reagent tablet is weighed, sampled and dissolved into a water solvent, and its ion concentration, pH value and related determinations are chemically analyzed to further test the stability and reliability of the reagent tablet.

Embodiment 2

[0016] Preparation of 20×SSC (hybridization buffer) solid dose. Adopt the same raw material ratio as the liquid agent, NaCl:sodium citrate=175:88 by weight. Accurately weigh the above-mentioned raw material particles with a precision balance according to the above-mentioned proportion, grind and pulverize them, and sieve them to 20 meshes, and add microcrystalline fiber and 1 / 10 paraffin wax accounting for 1 / 20 of the total weight of the raw materials. The above raw materials are mixed, placed in a tablet machine and pressed into reagent tablets with a size of 0.5 cm or 1 cm or 2 cm or 5 cm. Finally, let it dry. The pressed reagent disc is weighed, sampled and dissolved into a water solvent, and its ion concentration, pH value and related determinations are chemically analyzed to further test the stability and reliability of the reagent disc.

Embodiment 3

[0018] Preparation of 20×SSPE (hybridization buffer) solid dose. Use the same raw material ratio as the liquid agent, NaCl: Na 2 HPO 4 :EDTANa 2 =174:27.6:7.4 by weight. Accurately weigh the above-mentioned raw material particles with a precision balance according to the above-mentioned ratio, grind and pulverize, and sieve to 10 meshes, add microcrystalline fiber accounting for 1 / 10 of the total weight of the raw materials, and lubricants and paraffin wax accounting for 1 / 10 of the total weight of the raw materials. Mix the above-mentioned raw materials, put them in a tablet press, and press them into reagent discs of a certain size. Finally, let it dry. The pressed reagent tablet is weighed, sampled and dissolved into a water solvent, and its ion concentration, pH value and related determinations are chemically analyzed to further test the stability and reliability of the reagent tablet.

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Abstract

A solid tablet for buffering liquid for experiment is prepared through proportionally mixing all raw materials together, pulverizing, sieving by proper granularity, adding microcrystal fibres, disintegrant and adhesive, tabletting, dissolving the specimen tablet in water, analyzing its ionic concentration, pH value and relative parameters, and testing its stability and safety.

Description

technical field [0001] The present invention relates to the preparation method of experimental buffer solution and dosage form Background technique [0002] Buffers are widely used in various experiments in life science research, such as Northern-bltting, Southe-blotting, Western-blotting, immunohistochemistry, DNA Chip, protein chip and other experiments. However, the currently used buffer solution for experiments is mainly for temporary preparation or purchase of liquid preparations by laboratory personnel, and in the process of temporary preparation, the weighing of raw materials is inaccurate and cumbersome, and there are often differences in weighing from person to person Or some errors, resulting in less accurate experimental buffer. However, a small amount of commercially available liquid agents require containers such as glass bottles, which take up a lot of space, are inconvenient for transportation and storage, and the liquid agents are not very stable. Contents...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/52G01N37/00
Inventor 唐发清
Owner 长沙福滋堂生物技术开发有限公司