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Process for promoting generation of 10-hydroxycamptothecin in the bud of camptotheca seed

A technology of hydroxycamptothecin and seeds, applied in the fields of seed and rhizome treatment, organic chemistry, application, etc., can solve the problems of high cost, complicated process, large toxic and side effects, etc., and achieve the effect of low cost

Inactive Publication Date: 2004-03-17
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of artificially synthesizing 10-hydroxycamptothecin is that it has large toxic and side effects, and the process of converting camptothecin into 10-hydroxycamptothecin using sterile flavomycin is complicated and costly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Put 2 kilograms of camphoria seeds in hydroponics at room temperature for 192 hours to germinate them, place the germinated camphoria seeds in an ultrasonic device, add 4 kilograms of water to submerge the seeds, and under the conditions of frequency 20KHz and temperature 25°C, After ultrasonic radiation for 20 minutes, the radiated camptothecin seed buds were placed in a homogenizer with 5 liters of 70% ethanol added in advance to crush the seed bud tissue, homogenate and filter to obtain a filtrate containing 10-hydroxycamptothecin . The filtrate is centrifuged, and the supernatant is taken, and measured by high performance liquid phase, the content of 10-hydroxycamptothecin in the filtrate is 52.79mg / l, and the content of 10-hydroxycamptothecin has increased 20 times than the dry camptothecin seeds. Example 2

Embodiment 2

[0009] Put 10 kg of philodendron seeds in hydroponics at room temperature for 750 hours to germinate them, put the germinated philodendron seeds in an ultrasonic device, add 25 kg of water to submerge the seed buds, and ultrasonicate at a frequency of 40KHz and a temperature of 20°C After irradiating for 30 minutes, the radiated camptothecin seed buds were placed in a homogenizer pre-added with 25 liters of 85% ethanol to crush, homogenize and filter the seed bud tissues to obtain a filtrate containing 10-hydroxycamptothecin. The filtrate is centrifuged, and the supernatant is taken, and measured by high performance liquid phase, the content of 10-hydroxycamptothecin in the filtrate is 26.39mg / l, and the content of 10-hydroxycamptothecin has increased 25 times than the dry camptothecin seeds.

Embodiment 3

[0011] Put 15 kg of philodendron seeds in hydroponics at room temperature for 720 hours to germinate them, place the germinated philodendron seeds in an ultrasonic device, add 30 kg of water to submerge the seed buds, and ultrasonicate at a frequency of 40KHz and a temperature of 20°C After irradiating for 10 minutes, the radiated camptothecin seed buds were placed in a homogenizer pre-added with 30 liters of 95% ethanol to crush, homogenize and filter the seed bud tissues to obtain a filtrate containing 10-hydroxycamptothecin. The filtrate is centrifuged, and the supernatant is taken, and measured by high performance liquid phase, the content of 10-hydroxycamptothecin in the filtrate is 29.04mg / l, and the content of 10-hydroxycamptothecin has increased 22 times than the dry camptothecin seeds.

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Abstract

The present invention relates to the method of promoting the formation of 10-hydroxyl camptothecine in camptotheca seed sprout. The technological process includes water culture of camptotheca seed for sprouting, ultrasonic radiating sprouting camptotheca seed in an ultrasonic device with water, crushing and homogenating the seed sprout tissue in a homogenating machine, filtering to obtain filtrate of 10-hydroxyl camptothecine. The said process has the features of low cost and high yield of 10-hydroxyl camptothecine.

Description

Technical field [0001] The invention relates to a method for using environmental factors to affect secondary metabolites, in particular to providing a method for promoting the transformation of 10-hydroxycamptothecin in camptothecin seed buds by using ultrasound. Background technique [0002] At present, since the content of natural 10-hydroxycamptothecin in camptothecin seeds is very low, 10-hydroxycamptothecin is mainly obtained through artificial synthesis. On August 13, 1986, the Patent Office of the China Intellectual Property Office disclosed "Methods for the Production of 10-Hydroxycamptothecin by Biotransformation (CN 851 00520 A)", using sterile flavomycin to convert camptothecin into 10- Hydroxycamptothecin, the conversion rate is 50%. The disadvantage of artificially synthesizing 10-hydroxycamptothecin is high toxicity and side effects, and the process of converting camptothecin into 10-hydroxycamptothecin using sterile flavomycin is complicated and costly. Con...

Claims

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Application Information

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IPC IPC(8): A01C1/00A61P35/00C07D491/22
Inventor 祖元刚史权赵春建祖柏实唐中华付玉杰张宝友
Owner NORTHEAST FORESTRY UNIVERSITY
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