Rearing process for Shuangji cloned pig by gene engineering technology

A kind of genetic engineering and technical technology, applied in the field of "double muscle" cloned pigs, which can solve the problems of transgenic families that have not yet obtained homozygosity, and the characters cannot be stably inherited.

Inactive Publication Date: 2004-04-21
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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Problems solved by technology

Although some pigs transgenic for growth hormone have obtained good phenotypes, some traits cannot be inherited stably, and some have various aberrations due to the destruction of some endogenous genes or ectopic expression of transgenes due to the random integration of multiple copies of transgenes
More importantly, until now, no homozygous transgenic family has been obtained, which casts a shadow over the prospect of pig genetic engineering breeding

Method used

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  • Rearing process for Shuangji cloned pig by gene engineering technology
  • Rearing process for Shuangji cloned pig by gene engineering technology
  • Rearing process for Shuangji cloned pig by gene engineering technology

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Embodiment Construction

[0014] Materials and methods

[0015] 1. Reagents and medium: All reagents were purchased from Sigma-Aldrich Company; endonucleases and molecular biology reagents used were purchased from Promega Company; medium was purchased from Gibco Company.

[0016] 2. Gene cloning and identification: The full-length sequence of the porcine myostatin gene was screened out from the porcine genome library by conventional methods, and sequence analysis was carried out [Stratil A, Kopecny M. Genomic organization, sequence and polymorphism of the porcine myostatin (GDF8; MSTN ) gene. Anim Genet, 30(6): 468-70.].

[0017] 3. Construction of porcine myostatin gene targeting vector. A vector for targeting is constructed by conventional molecular cloning methods using appropriate restriction sites. After successful digestion and confirmation, the vector was linearized and quantified for later use.

[0018] 4. Obtain mature oocytes from sexually mature or pre-sexually mature s...

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Abstract

A genetic engineering technology for preparing the clonal pig by gene knock-out and somatic cell nucleus transplantation includes such steps as homoglous recombining to destroy the muscle growth inhibin gene of somatic cell, externally screening the targeted cells, and nucleus transplanting. Its advantage is stable inheritance of excellent characteristics.

Description

technical field [0001] The invention relates to a new method for pig breeding, in particular to a method for cultivating "double-muscle" cloned pigs with increased lean meat percentage, rapid growth and improved feed conversion rate by using transgenic technology. Background technique [0002] Pork is the favorite meat of our people. Except for some ethnic minorities and the Han population living in pastoral areas, the main meat that most people eat daily is pork. Along with improving day by day of people's living standard, people's demand for lean meat increases. As far as animal husbandry is concerned, obtaining new breeds of pigs with high lean meat percentage, rapid growth and improved feed conversion rate has become the goal that breeders have been striving for for many years. my country is a traditional pig-raising country and the country with the largest number of pigs in the world. Breeding pig breeds with good quality will have a huge and far-reaching impact on my...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
Inventor 林福玉邓继先黄培堂
Owner INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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