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Livestock sex control method by using sperm separation and embryo sex identification as basis

A control method and sperm technology, applied in biochemical equipment and methods, analysis materials, animal delivery, etc., can solve the problems of poor accuracy, no effect, and damage to sperm motility in separating sperm

Inactive Publication Date: 2004-10-13
内蒙古赛科星繁育生物技术(集团)股份有限公司
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  • Abstract
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Problems solved by technology

However, the above-mentioned methods were basically negated by the experimental results of most researchers in the 1990s due to poor or no effect on the accuracy of sperm separation, and damage to sperm motility, so they could not be used as a method. Practical technology applied to production practice

Method used

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  • Livestock sex control method by using sperm separation and embryo sex identification as basis
  • Livestock sex control method by using sperm separation and embryo sex identification as basis
  • Livestock sex control method by using sperm separation and embryo sex identification as basis

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Embodiment Construction

[0025] As shown in the figure: a livestock sex control method based on sperm separation and embryo sex identification, comprising the following steps:

[0026] (1) Sperm separation and cryopreservation:

[0027] A, take 1 milliliter of fresh semen from the normal kind of bull, use phosphate buffer solution PBS-0.05%PVP, centrifuge and wash 2 times, 2000 revs, 5 minutes, then use described phosphate buffer solution to adjust the concentration of sperm to 100×10 6 1 / ml, each ml of fresh semen can prepare 10-15 ml of diluted semen with the above concentration;

[0028] B. Add 10 μg / ml fluorescent dye Hoechst 33342 to the sperm fluid, stain DNA at 37°C for 30 minutes, then centrifuge and wash twice with phosphate buffer PBS-0.05% PVP, 2000 rpm, 5 minutes;

[0029] C. Readjust the sperm concentration to 100×10 6 Sperm per milliliter, then loaded into the flow cell separator, separated at a speed of 3000 sperm per second, the separated X sperm positively charged and Y sperm negati...

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Abstract

The invention relates to a cattle sex control method based on sperm separation and embryo sex identification, including the steps of sperm separation and freezing storage, and sex controlled embryo production and embryo sex identification, where the fist step includes fresh sampling sperm sampling, sperm processing ahead of separating, sperm separation processing, and freezing storage; the second step includes embryo production (in vivo or tube technique), embryo cutting, and embryo sex identification by PCR.

Description

technical field [0001] The invention belongs to a domestic animal sex control method, in particular to a domestic animal sex control method based on sperm separation and embryo sex identification. Background technique [0002] The sex of mammals (including domestic animals) is determined by the X and Y sperm in the semen at the time of fertilization. The combination of X sperm and egg produces a female individual, and Y sperm leads to the generation of a male individual. In the normal livestock semen, the proportion of X sperm and Y sperm is equal, so the male and female in the offspring account for about 50% respectively. For actual industrial operation, if X sperm and Y sperm can be separated, the sex of livestock can be selected according to production needs. For example, beef cattle breeders require to increase the birth rate of male calves as much as possible, while dairy cattle operators think that having multiple female calves is OK. Get greater economic benefits. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/00C12Q1/68G01N33/00G01N33/50G01N33/68
Inventor 李喜和
Owner 内蒙古赛科星繁育生物技术(集团)股份有限公司
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