Method for determining the existence and/or content of an analyte in a blood sample, and detecting element

A technology for detecting elements and analytes, which is applied in the preparation of test samples, analytical materials, measuring devices, etc., and can solve problems such as interfering with the accurate determination of analytes

Active Publication Date: 2004-12-29
BAYER HEALTHCARE LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Variations in the hematocrit of whole blood samples used in diagnostic assays are known to interfere with the accurate determination of analytes

Method used

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  • Method for determining the existence and/or content of an analyte in a blood sample, and detecting element
  • Method for determining the existence and/or content of an analyte in a blood sample, and detecting element
  • Method for determining the existence and/or content of an analyte in a blood sample, and detecting element

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Embodiment Construction

[0019] In accordance with the present invention, there is provided a method of assaying an analyte in a blood sample in which the mean cell volume of the blood sample is reduced to reduce the interfering effect of hematocrit on the accuracy of the assay. According to the method of the present invention, at some point during the diagnostic procedure, the average cell volume of the sample is reduced by treating it with a non-lysing hypertonic saline composition. The term "hypertonic salt composition" as used in this patent means any composition of one or more organic or inorganic salts or mixtures thereof sufficient to create a higher osmotic pressure in a blood sample than isotonic fluid. The term "non-lysed" as used in this patent means that the composition will not cause significant lysis of the red blood cells in the sample when analyzed by the method of the present invention.

[0020] In particular, the methods of the present invention comprise the steps of: (a) providing a...

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Abstract

A method for determining the presence and / or amount of an analyte in a sample of whole blood comprises the step of treating the sample with a nonlytic hypertonic salt composition to reduce the hematocrit by reducing the size of the red blood cells. In optical detection systems, the smaller red blood cells create greater scatter, which allows a more accurate correction to be applied in a dual-wavelength detection system. In electrochemical detection systems, as well as in optical detection systems, the smaller red blood cells provide less obstruction to the diffusion of analyte and reagents in the sample, to facilitate the reactions thereof.

Description

technical field [0001] The present invention relates to a method of determining the presence and / or amount of an analyte in a blood sample by reducing the influence of hematocrit in the determination to improve its precision and accuracy. The invention also relates to a detection element for carrying out the method, and a detection kit comprising the detection element. Background technique [0002] The ability to quantify the chemical and biochemical constituents of whole blood is becoming increasingly important to medical science. This capability is important when determining the effects of noxious substances, anesthetics, therapeutic drugs, and in diagnostics. In some applications, it is important that a layperson be able to perform the test outside of the laboratory and obtain accurate and rapid results. For example, diabetics must measure glucose levels in their own blood samples several times a day and adjust food and medication accordingly. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/00G01N15/02G01N21/31G01N21/49G01N21/59G01N21/86G01N27/26G01N27/44G01N33/487G01N33/49
CPCG01N15/0205G01N21/8483G01N2015/0073G01N21/314G01N27/3271
Inventor K·L·马福尔特
Owner BAYER HEALTHCARE LLC
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