Radio rhizobia for reducing endemic nitrosamine content fortobacco and its use

A technology of irradiating rhizobia and nitrosamines, which is applied in the biological field and can solve problems that have not been widely applied

Inactive Publication Date: 2005-02-16
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitations of these methods, they have not been widely applied at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Inoculate Rhizobium radiobacter (Rhizobium radiobacter) KenLXR34 strain on the slant of the test tube. The medium formula is: 1.2% casein, 0.8% soybean peptone, 0.5% sodium chloride, 1.7% agar, pH7.5. Constant temperature culture at 30°C for 1 Day, get test tube species.

[0067] The test tube is inoculated into a 500ml Erlenmeyer flask (200ml per bottle) liquid medium, and the medium formula is: casein 1.5%, soybean peptone 0.5%, sodium chloride 0.5%, dipotassium hydrogen phosphate 0.025%, glucose 0.3%, pH7.5. Cultivate the inoculated triangular flask at 30°C on a shaking table (180 rpm) for 2 days, and when the bacterial concentration reaches ≥108 viable bacteria per ml, it can be applied to tobacco, which can reduce the risk of burley tobacco. TSNA content.

Embodiment 2

[0069] Basically the same as Example 1, the difference lies in the formula of the liquid medium, which is: 0.5% beef extract, 1.2% peptone, 0.5% sodium chloride, pH 7.0.

Embodiment 3

[0071] Basically the same as in Example 1, the difference lies in the formula of the liquid medium, which is: 4.5% nutrient agar powder, and the pH is natural.

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PUM

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Abstract

The invention relates to a kind of radiating legume bacteria that can reduce the content of nitrosamine in tobacco and its application. The radiating legume bacteria, Rhizobium radiobacter KenLXR34, of the invention has been kept by common microorganism center of China microorganism storing management committee, its number is CGMCC NO:0986. the main character of the invention follows this, screen out the radiating legume bacteria, KenLX34, which can reduce the content of nitrosamine in tobacco markedly, after fermenting culturing in liquid, it can be used for tobacco to reduce the content of TSNA in smoke. If dip footstalk in strain hanging liquid, the depression ratio is highest, it can reach 98.28%. so the invention has favourable and latent application.

Description

Technical field: [0001] The invention relates to a radiorhizobium strain and its application in reducing the content of tobacco-specific nitrosamines, belonging to the field of biological technology. Background technique: [0002] Tobacco-specific nitrosamines (TSNA) are a group of carcinogens found only in tobacco and its products, mainly including N-nitrosonornicotine (NNN), 4-(N-formazine 4 Among them, NNN and NNK have significant carcinogenic effects, while NAB and NAT have no obvious carcinogenic effects. Burley tobacco had the highest content of TSNA in different tobacco types, and the content of oriental tobacco and flue-cured tobacco was lower. Studies have shown that the main precursors of TSNA are alkaloids, nitrate, and nitrite. There is no TSNA in the green tobacco leaves before harvest, and they are produced during the modulation and fermentation process after harvest. Over the past 20 years, TSNA has become one of the most focused areas of tobacco scientific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20C12N1/20
Inventor 祝明亮李天飞汪安云王东丹
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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