Biological chip time division complex multiple fluorescent simultaneously detecting method and device

A biochip and time-division multiplexing technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., to achieve the effects of less time-consuming detection, simple instrument maintenance, and low instrument cost

Inactive Publication Date: 2005-03-02
UNIV OF SHANGHAI FOR SCI & TECH
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Problems solved by technology

[0006] The present invention aims to solve the technical problem of simultaneously detecting the presence of multiple fluorescences in existing biochip laser confocal scanners, and provides a method and device that can simultaneously excite multiple fluorescent substances and detect corresponding fluorescent signals

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  • Biological chip time division complex multiple fluorescent simultaneously detecting method and device
  • Biological chip time division complex multiple fluorescent simultaneously detecting method and device
  • Biological chip time division complex multiple fluorescent simultaneously detecting method and device

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0022] This embodiment is a method and device for simultaneous detection of biochips labeled with two fluorescent substances, CY3 and CY5. The absorption peak wavelength of the CY3 fluorescent substance is about 530nm, so a semiconductor laser with a wavelength of 532nm is used to pump a frequency-doubled laser as an excitation light source, and the peak wavelength of fluorescence generated by excitation is 570nm. The absorption peak wavelength of the CY5 fluorescent substance is about 630nm, so a semiconductor laser with a wavelength of 635nm is used as the excitation light source, and the fluorescence peak wavelength generated by excitation is 670nm. Semiconductor lasers have a very high modulation frequency, which can meet the actual modulation needs. In this embodiment, biochips with two fluorescent substances are used, so two corresponding lasers ...

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Abstract

The invention discloses a biological chip time mulitiplex multi-fluorescence simultaneous detection method and apparatus. Its method has the following steps: several laser maser for exciting different fluorescent substances and corresponding reflection holographic optical filter are installed in scanner; after laser maser pulse modulation to laser maser, optical pulse collimation through separate collimation enters the same measuring optical circuit to excite in turn biological chip of several fluorescent substances which creates fluorescence signal with different phase; after being collected and focusing, entering photomultiplier through pinhole to be transformed into pulse electric signal which is fluorescence signal for diplex corresponding to fluorescence signal with different phase; releasing diplex of signal is realized by multiplying unit obtaining separate fluorescence signal exciting; each fluorescence signal is transformed through A/D, transferred to computer used to do two-dimensional scan to biological chip. The invention uses biological chip laser scanner and time mulitiplex method to realize detection of fluorescence signal and exciting to multi kind of fluorescent substances with only one receive system and once scan.

Description

technical field [0001] The invention belongs to the field of biological optical detection, in particular to a detection method and device of a biological chip. Background technique [0002] The existing biochip detection methods mainly include CCD imaging method and laser confocal scanning method. [0003] The CCD imaging method uses an excitation light source to irradiate the biochip at the same time, filters out the excitation light at the detection end, and only allows the fluorescence to pass through, and images on the CCD through the imaging system, and the two-dimensional fluorescence signal is recorded by the CCD. The CCD imaging method can obtain the information of one area on the biochip in one measurement, so it is characterized by fast detection speed. The disadvantage is that the lateral resolution is relatively low; in order to improve the lateral resolution, the magnification of the imaging system needs to be increased, so the field of v...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 马军山袁武付东翔侯琳琳陈家璧
Owner UNIV OF SHANGHAI FOR SCI & TECH
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