Application of 20(S)-ginsenoside-Rg#[3] in the preparing process of medicine for treating or preventing cerebrovascular disease
A cerebrovascular disease and ginsenoside technology, which is applied in the application field of 20(S)-ginsenoside-Rg3 in the preparation of medicines for treating or preventing cerebrovascular diseases
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Examples
preparation example 1
[0014] Preparation Example 1: 20(S)-Ginsenoside Rg 3 And 20(R)-Ginseng Shake Rg 3 preparation of
[0015] Take total saponins of Panax notoginseng (or total saponins of ginseng) as raw material, add 50% acetic acid aqueous solution, heat in a water bath at 70°C for 6 hours, cool, add 5% sodium carbonate solution, extract the solution with n-butanol, evaporate the extract to dryness, Go up silica gel column, take chloroform-methanol as eluent, carry out gradient elution, obtain 20(S)-ginsenoside Rg 3 and 20(R)-ginsenoside Rg 3 The crude product, through repeated silica gel column chromatography, obtains 20 (S)-ginsenoside Rg respectively 3 and 20(R)-ginsenoside Rg 3 . And according to the literature [Tang Minghui, Jiang Zhihong, Zhao Zhongzhen, et al. Separation and detection of 20(S)-ginsenoside Rg3 and 20(R)-ginsenoside Rg3 by HPLC. Chinese herbal medicine. 2003, 35(3): 208-282.] detection method described above.
preparation example 2
[0016] Preparation Example 2: 20(S)-Ginsenoside Rg for Injection 3 Preparation
[0017] Weigh 0.50g 20(S)-ginsenoside Rg 3 , add 50g PEG-400 to dissolve the saponin; prepare 50g pH5.5 phosphate buffer solution, weigh 0.02g sodium bisulfite and add it to the above phosphate buffer solution; add the buffer solution to the PEG solution of saponin, Mix well, add 0.01% activated carbon for needles and keep warm at 100°C for 30 minutes, G 3 Filtration with a vertically fused glass funnel and a 0.22um microporous membrane. The filtered medicinal solution is packaged into 50 injections of 2ml each.
[0018] Preparation 3: 20(S)-ginsenoside Rg for injection 3 Preparation of freeze-dried powder
[0019] Weigh 0.50g 20(S)-ginsenoside Rg 3 , add 50g PEG-400 to dissolve the saponin. Prepare 50g of pH5.5 phosphate buffer, weigh 0.02g of sodium bisulfite and add it to the above phosphate buffer; add the buffer to the PEG solution of saponin, mix well, add 0.01% activated carbon for nee...
preparation example 4
[0020] Preparation Example 4: 20(R)-Ginsenoside Rg for Injection 3 Preparation
[0021] Weigh 0.50g 20(R)-ginsenoside Rg 3 , add 50g PEG-400 to dissolve the saponin. Prepare 50g of pH5.5 phosphate buffer, weigh 0.02g of sodium bisulfite and add it to the above phosphate buffer; add the buffer to the PEG solution of saponin, mix well, add 0.01% activated carbon for needles, 100°C Keep warm for 30 minutes, G 3 Filtration with a vertically fused glass funnel and a 0.22um microporous membrane; the filtered medicinal solution is divided into 2ml / vials, and freeze-dried by a freeze dryer to make a freeze-dried powder for injection.
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