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Combined application of gp64 gene and p35 gene of baculovirus

A baculovirus and gene technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem of no recombinant virus improvement, etc.

Inactive Publication Date: 2005-08-17
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, the research on the gp64 gene and p35 gene of AcMNPV at home and abroad is still in the stage of further analysis of functions, and there is no report on the application of recombinant virus improvement, and there is no example of the combination of the above two genes

Method used

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  • Combined application of gp64 gene and p35 gene of baculovirus
  • Combined application of gp64 gene and p35 gene of baculovirus
  • Combined application of gp64 gene and p35 gene of baculovirus

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Embodiment Construction

[0017] In the present invention, by jointly expressing the gp64 gene and the p35 gene of Autographa californica virus (Autographa californica MNPV, AcMNPV), it is found that the recombinant virus using the gp64 gene and the p35 gene of AcMNPV has advantages in terms of virus infectivity and host adaptation range. Significant improvement and improvement, it is speculated that the gp64 gene and p35 gene of BmNPV and OpMNPV may also have similar effects.

[0018] The present invention will be further described below in conjunction with example and accompanying drawing.

[0019] 1. gp64 and p35 genes of AcMNPV

[0020] The nucleotide sequences of gp64 and p35 genes are shown in Fig. 1 and Fig. 2 respectively, and their index numbers in the GenBank database are NC_001623. Among them: the coding region of the gp64 gene is 1501 nucleotides long, encoding a 58.5 kDa envelope glycoprotein product; the p35 gene coding region is 841 nucleotides long, encoding an anti-apoptotic protein p...

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Abstract

The present invention is the joint application of baculovirus gp64 gene and p35 gene. The gp64 gene and p35 gene of baculovirus AcMNPV, or Autogrpha California multiple nuclear polyhedrosis virus, are jointly expressed in the joint application. The gp64 gene and p35 gene of baculovirus AcMNPV have index number in GenBank of NC001623. The gp64 gene and p35 gene combination is used in the transfer vector. The present invention provides important theoretic base for constituting recombinant virus with expanded host domain and provides one hopeful way for developing recombinant virus pesticide with wide spectrum pesticidal activity, and improving and developing genetically the expression vector system.

Description

technical field [0001] The invention relates to the combined application of gp64 and p35 genes of a baculovirus, i.e. Autographa californica nuclear polyhedrosis virus (AcMNPV). Background technique [0002] Baculovirus is a pathogenic virus that specifically infects and kills arthropods, mainly Lepidoptera insects. Because baculovirus is harmless to humans and animals, has no environmental retention, and pests are not easy to develop resistance, it was recommended by WHO in 1973 as The control of harmful insects has been widely used so far. It is an ideal substitute for chemical pesticides and has broad market prospects. However, due to the shortcomings of narrow insecticidal spectrum and slow insecticidal speed of baculovirus wild-type virus insecticides, scientific researchers have improved the insecticidal efficacy of wild-type viruses through genetic engineering. However, at present, the improvement of recombinant viruses is mainly focused on increasing the insecticida...

Claims

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Application Information

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IPC IPC(8): C07H21/00C12N7/01C12N15/33C12N15/63C12N15/866
Inventor 王华林王慧媛邓菲袁丽胡志红
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI