Method for preparing recombined human atrial natriuretic peptide rhANP by using ferment in high density
A high-density fermentation, human-friendly technology, applied in fermentation and other directions, can solve the problems of limiting the industrial application of ANP, difficulty in ANP expression and purification, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0033] The present invention is further described below in conjunction with embodiment.
[0034] The preparation method of the present embodiment comprises the following process steps:
[0035] A. The slant strains are expanded and cultivated to make seed liquid
[0036] The glycerol-producing strains preserved at -70°C were inoculated in a test tube at a ratio of 1% (3ml LB / Amp+medium), and slant strains were made under the culture conditions of 30°C, 300rpm, and a culture cycle of 15 hours; Inoculate in a small conical flask (20ml LB / Amp+medium) at a ratio of 1%, culture at 30°C and 300rpm for 8 hours; then inoculate a large conical flask at a ratio of 1% (1500ml 2-YT / Amp+medium) , 30° C., 300 rpm for 15 hours.
[0037] B. Fermentation
[0038] The above seed solution was inoculated into 30L fermentation medium at a ratio of 3%. The composition of the fermentation medium is:
[0039] Glycerin 5g / L, tryptone 7g / L, yeast powder 3g / L, KH 2 PO 4 7g / L,
[0040] K 2 HPO...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com