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Artificial bonegraft and its preparing method

A graft and artificial bone technology, applied in the field of artificial bone grafts and its preparation, can solve the problems of difficulty in obtaining osteoblasts and few sources, achieve the effect of solving the source of seed cells and adapting to large-scale production and utilization

Inactive Publication Date: 2005-12-21
上海蓝融股权投资基金管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above methods have the following disadvantages: the acquisition of osteoblasts is difficult, the source is small, and new injuries are caused to patients in different degrees, which limits further application.

Method used

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  • Artificial bonegraft and its preparing method
  • Artificial bonegraft and its preparing method
  • Artificial bonegraft and its preparing method

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Embodiment Construction

[0033] The present invention is further specifically described below in conjunction with specific examples, but is not limited thereto. This fabrication technique is still clear to those skilled in the art.

[0034] (1) Sampling and pretreatment of adipose tissue: the adipose tissue is separated from the living body. Pack the separated adipose tissue into a 50ml centrifuge tube under aseptic conditions and bring it back to the laboratory within 2 hours. Then rinse repeatedly with sterile PBS until the aspirate solution becomes clear to remove blood cells. Add an equal volume of 0.01-0.5% type I collagenase, digest in a constant temperature shaker at 37°C for 20-100 minutes, and centrifuge at 1200rpm for 10 minutes to obtain a high-density cell sediment, gently pour off the supernatant and floating yellow tissue, Shake the cells and add DMEM and 10% FBS to resuspend the cells.

[0035] (2) Inoculation: Draw a small amount of cell suspension and dilute it with 4% acetic acid ...

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Abstract

The present invention relates to field of preparation technology of product related to biological medicine engineering tissue transplantation, in the concrete, it relates to an artificial bone graft and its preparation method. The described graft contains pharmaceutically-acceptable degradable material and fatty stem cell. Its preparation method includes the following steps: (1) using culture solution DMEM to make culture and amplification of fatty stem cell, before inoculation using osteogenetic induction solution to make culture; (2) inoculating fatty stem cell in the pharmaceutically-acceptable bio-degradable material; and (3) making culture under the proper growth condition so as to form fatty stem cell-pharmaceutically-acceptable bio-degradable material compound.

Description

[technical field] [0001] The invention relates to the technical field of preparation of products related to biomedical engineering tissue transplantation, in particular to an artificial bone graft and a preparation method thereof. [Background technique] [0002] At present, due to factors such as immune rejection, the clinical cases of using tissue engineering to repair bone tissue defects reported in the literature all use the host's own cells as the seed cells of tissue-engineered bone, which greatly limits the large-scale production of tissue products. Can do some individualized treatment. In 2001, it was reported that allogeneic engineered bone constructed with osteoblasts as seed cells did not see obvious lymphocyte infiltration and new bone formation in the allogeneic body. (Cui Pengcheng et al. Constructing allogeneic engineered bone to repair defect by tissue engineering method, Modern Rehabilitation 2001VoL5 No.22P44-45). However, the above methods have the follow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 丁勃元
Owner 上海蓝融股权投资基金管理有限公司