Method of improving lactic acid bacteria freeze drying activity using preculturing mode

Abstract

The invention relates to a method to improve lactic acid bacteria freeze-dry activity that belongs to acidophilus milk freeze-dry ferment and lactic acid bacteria active fungus technology field. It mixes the lactic acid bacteria body before freeze-dry and protection agent, lays at 4 degree centigrade for 30 minutes to balance, under a certain temperature, after a certain time of pre-cultivating, then freezing, drying and storing. The method could improve the freeze-dry activity of lactic acid bacteria.

Description

technical field

[0001] The invention discloses a method for improving the freeze-dried activity of lactic acid bacteria by means of pre-cultivation, which belongs to the technical field of yogurt freeze-dried starter and live lactic acid bacteria preparations. Background technique

[0002] Due to the problems of cost and industrialization, the most commonly used method for cell collection is centrifugation, that is, the centrifugal force of the centrifuge is used to deposit the cells. The purpose is to separate the cells from the culture medium and metabolites, and to minimize For the damage of bacteria, this method is simple, fast, and has a large amount of treatment, which is suitable for commercial production. After the bacteria are enriched and cultured, collected by centrifugation and suspended with a protective agent, the general measure is to quickly pre-freeze at a low temperature, and freeze-dry after a certain period of time. From the step of suspending the bacter...

Examples

Embodiment 1

[0013] The strains were Lactobacillus delbrueckii subsp. Bulgaricus (Lactobacillus delbrueckii subsp. Bulgaricus) CTCC6047 and Streptococcus thermophilus (Streptococcus thermophilus) CTCC6038 in a ratio of 1:1 and cultured in the fermentation medium with 2%. The conventional method is through enrichment culture, and the bacteria are collected after centrifugation, added with the above protective agent to suspend, placed at 4°C for 30 minutes and then pre-cultured. Select 20°C, 30°C, 42°C, 55°C for the pre-cultivation temperature and 1 hour, 2 hours, and 3 hours for the pre-cultivation time, and conduct primary selection to investigate the changes in pH and bacterial count during the culture process; investigate the pre-culture The survival rate and clotting time of the treated cells after freeze-drying. The results show that: at the growth temperature of 20°C, the indicators of the bacteria are very close, and the pH and the number of bacteria have no change. With the extensio...

technical field

[0001] The invention discloses a method for improving the freeze-dried activity of lactic acid bacteria by means of pre-cultivation, which belongs to the technical field of yogurt freeze-dried starter and live lactic acid bacteria preparations. Background technique

[0002] Due to the problems of cost and industrialization, the most commonly used method for cell collection is centrifugation, that is, the centrifugal force of the centrifuge is used to deposit the cells. The purpose is to separate the cells from the culture medium and metabolites, and to minimize For the damage of bacteria, this method is simple, fast, and has a large amount of treatment, which is suitable for commercial production. After the bacteria are enriched and cultured, collected by centrifugation and suspended with a protective agent, the general measure is to quickly pre-freeze at a low temperature, and freeze-dry after a certain period of time. From the step of suspending the bacter...

Examples

Embodiment 1

[0013] The strains were Lactobacillus delbrueckii subsp. Bulgaricus (Lactobacillus delbrueckii subsp. Bulgaricus) CTCC6047 and Streptococcus thermophilus (Streptococcus thermophilus) CTCC6038 in a ratio of 1:1 and cultured in the fermentation medium with 2%. The conventional method is through enrichment culture, and the bacteria are collected after centrifugation, added with the above protective agent to suspend, placed at 4°C for 30 minutes and then pre-cultured. Select 20°C, 30°C, 42°C, 55°C for the pre-cultivation temperature and 1 hour, 2 hours, and 3 hours for the pre-cultivation time, and conduct primary selection to investigate the changes in pH and bacterial count during the culture process; investigate the pre-culture The survival rate and clotting time of the treated cells after freeze-drying. The results show that: at the growth temperature of 20°C, the indicators of the bacteria are very close, and the pH and the number of bacteria have no change. With the extensio...