Medium for inducing cucumber macrospore in vitro haploid embryogenesis and its uses

A technology for embryogenesis and megaspores, which can be applied to plant cells and other directions, and can solve problems such as large differences in type.

Inactive Publication Date: 2006-02-22
TIANJIN RES INST OF VEGETABLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it has the following deficiencies: ①The test materials are all European smooth type cucumbers, and the genotypes of European smooth type cucumbers and Chinese prickly tumor type cucumbers are quite different. There are limitations; ②Cucumber haploid embryogenesis induction medium must be adjusted according to the parthenocarpy ability of the material: varieties with strong parthenocarpy ability need to add cytokinin and low concentration of auxin; non-parthenocarpic varieties need to add Add auxin
At present, there are no other studies on cucumber haploid induction at home and abroad

Method used

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  • Medium for inducing cucumber macrospore in vitro haploid embryogenesis and its uses

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Experimental program
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Effect test

Embodiment 1

[0020] A medium for inducing haploid embryogenesis of cucumber megaspores in vitro, comprising the following components: KNO 3 750mg, CaCl 2 .H 2 O is 165 mg, MgSO 4 .7H 2 O is 75 mg, KH 2 PO 4 170mg, (NH 4 ) 2 SO 4 120mg, MnSO 4 10.5mg, ZnSO 4 8.6 mg; H 3 BO 3 7.2mg, KI 0.83mg, FeSO 4 .7H 2 O 27.8 mg; Na 2 EDTA.2H 2 O 37.3mg, inositol 130mg, vitamin B 1 0.5mg, vitamin B 6 1.0 mg of niacin, 2.0 mg of niacin, 2.0 mg of glycine, 0.4 mg of indole acetic acid, 2.5 mg of 6-benzyl adenine, 30 g of sucrose, 7 g of agar, pH 5.8, add water to 1 liter, follow the conventional method Make culture medium.

Embodiment 2

[0022] A medium for inducing haploid embryogenesis of cucumber megaspores in vitro, comprising the following components: KNO 3 550mg, CaCl 2 .H 2 O is 165 mg, MgSO 4 .7H 2 O is 190mg, KH 2 PO 4100mg, (NH 4 ) 2 SO 4 120mg, MnSO 4 10.5mg, ZnSO 4 8.6 mg; H 3 BO 3 7.2mg, KI 0.83mg, FeSO 4 .7H 2 O 27.8 mg; Na 2 EDTA.2H 2 O 37.3mg, inositol 130mg, vitamin B 1 0.5mg, vitamin B 6 1.0 mg of niacin, 2.0 mg of niacin, 2.0 mg of glycine, 0.3 mg of naphthaleneacetic acid, 2.0 mg of kinetin, 30 g of sucrose, 7 g of agar, pH 5.8, add water to one liter, and prepare the culture medium according to the conventional method.

Embodiment 3

[0024] A medium for inducing haploid embryogenesis of cucumber megaspores in vitro, comprising the following components: KNO 3 300mg, CaCl 2 .H 2 O is 300mg, MgSO 4 .7H 2 O is 50 mg, KH 2 PO 4 150mg, (NH 4 ) 2 SO 4 400mg, MnSO 4 5mg, ZnSO 4 5 mg; H 3 BO 3 6mg, KI 1.0mg, FeSO 4 .7H 2 O 27.8 mg; Na 2 EDTA.2H 2 O 37.3mg, inositol 200mg, vitamin B 1 0.1mg, vitamin B 6 0.5mg of niacin, 3.0mg of niacin, 1.0mg of glycine, 0.2mg of naphthaleneacetic acid, 1.0mg of kinetin, 30g of sucrose, 7g of agar, pH 5.8, add water to 1 liter, and prepare the medium according to the conventional method.

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Abstract

Disclosed is a culture medium for inducing cucumber macrospore in vitro monoploid embryogenesis, which comprises different contents of KNO3, CaCl2*H2O, MgSO4*7H2O, KH2PO4, (NH4)2SO$, MnSO4, ZnSO4, H3BO3, KI, FeSO4*7H2O, Na2EDTA*2H2O, inositol, vitamins B1, vitamins B6, nicotinic acid, glycine, auxin, cytokinin, sucrose and agar.

Description

Technical field: [0001] The invention relates to a culture medium for inducing haploid embryogenesis in plant cell engineering. In particular, it relates to a medium for inducing haploid embryogenesis in vitro of megaspores and its application in inducing haploid embryogenesis in vitro of cucumber megaspores. Background technique: [0002] Cucumber is one of the vegetable varieties that Chinese people like to eat. Cucumber hybrid breeding has made great achievements. Four generations of cucumber varieties have been bred successively: Jinyan, Jinza, Jinchun, and Jinyou, which account for more than 80% of the country's cucumber planting area. The current conventional hybrid breeding method needs to cultivate inbred line parents with excellent traits first. Due to the continuous separation of hybrid offspring, continuous selfing and selection of at least 6 to 8 generations are required to obtain a stable inbred line, which generally costs 3 -4 years, it usually takes 5 to 8 y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 杜胜利魏爱民韩毅科张历王艳飞
Owner TIANJIN RES INST OF VEGETABLE
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