Trachyostracous mussel extract and its prepn and use

A thick-shelled mussel and a manufacturing method technology, which are applied in the directions of drug combination, medical raw materials derived from mollusks, respiratory system diseases, etc., to enhance the transformation ability of B lymphocytes, enhance the humoral immune function, and increase the transformation ability of T lymphocytes Effect

Inactive Publication Date: 2006-05-17
SHANGHAI CENT OF RES & DEV OF NEW DRUGS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of mussel substances in new drugs at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of the extract of embodiment 1 thick shell mussel

[0037]Collect fresh thick-shelled mussels with bright shells and roughly equal individual size and weight from the Zhoushan Islands in Zhejiang Province, my country, wash, shell, and wash away the sediment. Store in -20°C refrigerator. Take out the preserved thick-shelled mussels, thaw, boil for 1-2 hours, filter out the dry matter, and homogenize. Adjust the pH of the raw material slurry to 6.0, add 0.1% (ratio to raw material) Neutrase enzyme produced by Danish Novozymes, 45 ° C, stir for 3 hours; then boil, adjust the pH to 6.8, add 1% (ratio to raw material) Flavourzyme enzyme, 50 ℃, stirred for 3h, boiled. Obtain a hydrolyzate. Dilute the obtained hydrolyzate by 10 times, remove impurities with an ion exchange column equipped with weakly basic resin D303, and obtain a crude product with a pH of about 5.0. Concentrate the obtained crude product in a rotary vacuum concentrator at 50° C. to an ext...

Embodiment 2

[0038] Embodiment 2 The selection of mussel raw material

[0039] Raw materials were purchased from different origins. It is prepared into freeze-dried powder, or frozen after being shelled. Samples were prepared by the established production process, and the amino N, total N and in vivo activity were determined. Compare the advantages and disadvantages of each raw material, as shown in Table 1.

[0040] Out of the meat

Rate(%)

Dry matter contains

quantity(%)

N content

mg / mL acid hydrolysis

liquid

Amino N content

mg / mL acid solution

Hydrolysis rate = amino group

N / total N%

In vivo activity (flat

Average days of life)

blue mussel

Emerald Mussels

Thick shelled mussels

dried mussels

13.1

13.8

13.6

13.3

15.6

19.6

80.5

13.66

15.17

18.01

16.83

12.79

14.66

14.13

15.13

93.6

96.6

78.5

89.9

8.06

...

Embodiment 3

[0043] Example 3 Determination of amino acid content in samples

[0044] Get the extract of Example 1, measure and measure the amino acid content according to the commonly used RP-HPLC and OPA method in the art (Edited by Xia Qichang, "Protein Chemistry Research Technology and Progress", Beijing: Science Press, 1999: 30-32.) , the result is amino acid 12.5g / 100mL extract.

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PUM

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Abstract

The present invention relates to medicine preparation with animal material, and is especially trachyostracous mussel extract and its preparation and use. The trachyostracous mussel extract features its total nitrogen content of 12-16 mg / ml, protein concentration of 15-20 mg / ml, amino nitrogen content of 8.5-15 mg / ml and pH 5.0-6.0. The preparation process includes washing trachyostracous mussel, eliminating shell, homogenizing meat to form homogeneous slurry, hydrolysis, eliminating acid and impurity with weak alkaline resin, concentration and other steps. The trachyostracous mussel extract of the present invention may be used in preparing medicines for resisting influenza, resisting SARS and regulating immunity.

Description

technical field [0001] The present invention belongs to the field of medical preparations containing raw materials from animals other than mammals or birds or the extracts of their reaction products with unknown structures, in particular to the extract, production method and pharmaceutical application of thick-shelled mussels Applications. Background technique [0002] The ocean is a huge treasure house of medicine sources. So far, more than 1,000 new compounds have been obtained from marine animals and plants, more than half of which have pharmacological activities such as antitumor, antibacterial, and antiviral. The study of marine drugs has become the trend of modern biomedical research. Compared with other shellfish, mussels have high yield and low price. Mussel, synonym: shell vegetable, sea red, red clam, etc., Latin name: Mytilus edulis; English name: mussel. The main species are: 1. Blue mussel (Mytilus galloprovincilis Linaeus), which lives on rocky reefs near t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/618A61P11/10A61P29/00A61P43/00
Inventor 唐军汪兰英张灵霞杨秀珍
Owner SHANGHAI CENT OF RES & DEV OF NEW DRUGS
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