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Reagent kit and method for detecting early stage cancer

A technology for early detection and kits, which is applied in the fields of medicine and pharmacy, can solve the problems of high detection cost, inconvenient promotion, and inconvenient use, and achieve the effects of low cost, easy promotion, and easy acquisition

Inactive Publication Date: 2006-05-17
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Determination of the light absorption of NADH at 340nm requires a UV photometric analyzer or a special analyzer, and requires specialized technical personnel to operate, which is not very convenient to use, the detection cost is high, and it is not easy to promote

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0015] 1. Separation and extraction of L-fucose dehydrogenase

[0016] Extract L-fucose dehydrogenase from fresh pig liver: Homogenize 250 g of fresh pig liver with a tissue grinder, then add 1 L of 0.25 mol / L sucrose, centrifuge at 25,000 g for 20 min, discard the precipitate and take the supernatant, add 450 ml to saturate Ammonium sulfate, centrifuge at 25000g for 20min after standing for 15min, discard the precipitate and take the supernatant, add 400ml saturated ammonium sulfate, centrifuge at 25000g for 20min after standing for 15min, discard the supernatant to remove the precipitate, dialyze with 0.005M phosphate buffer saline (PBS), and take the dialysate Diethylaminoethyl (DEAE) cellulose column (DE 52 ), eluted with a gradient of 0.005mol / L phosphate buffered saline (PBS) containing 0.05-0.15mol / L sodium chloride (NaCl), and collected the eluted fraction of 0.1-0.15mol / L sodium chloride (NaCl) Concentrate, measure L-fucose dehydrogenase, and store in a -70°C refrige...

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PUM

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Abstract

The present invention discloses a kit for detecting early carcinoma and its method. Said kit includes solution-A, solution-B and solution-C. Its detecting method includes the following steps: firstly, extracting L-fucosedehydrogenase from pig liver, placing it in refrigerator with-79 dge.c for stand-by; secondly, adopting solution in the kit; then taking patientí»s urine, respectively adding solution-A, solution-B and solution-C, heat-insulating and observing reaction. Its detected result is visible to the naked eyes, and its detection effect is high, the positive rate of carcinoma of digestive tract can be up to 85-90%.

Description

technical field [0001] The present invention relates to the fields of medicine and pharmacy. More specifically, it relates to a kit for detecting early cancer, which is suitable for the detection of digestive tract cancer, and also relates to a detection method. Background technique [0002] According to statistics, there are about 1.6 million new cancer patients in my country every year, and about 1.3 million people die from cancer every year. Among the many causes of death among residents in large and medium-sized cities in my country, cancer is the first cause of death, and among various causes of death in rural areas, cancer is the second cause of death. For middle-aged and elderly people, cancer is a frequently-occurring disease and a common disease. According to the current medical level, more than 80%-90% of early cancer patients can be cured; however, there are fewer advanced cancer patients who can survive for more than 5 years after treatment. After the treatmen...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N1/34G01N33/50
Inventor 吴建国邬开朗刘芳朱应祝成亮
Owner WUHAN UNIV
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