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Bacterium for degrading metsulfuron-methyl pesticide residue and produced bacterium formulation

A technology of pesticide residue degradation and metsulfuron-methyl, which is applied in the field of high biotechnology, can solve the problems of limited use and narrow degradation spectrum of bacterial strains, and achieve the effects of reducing production and use costs, low production and use costs, and protecting the ecological environment

Inactive Publication Date: 2006-07-12
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

In the prior art, our research group already has an invention patent for degrading bacteria (ZL96106317.3, publication number: CN1145408A, publication date: March 19, 1997), which is a strain of Bacillus J5, which can effectively degrade methylamine Phosphorus, but the strain has a narrow degradation spectrum and can only degrade one pesticide, methamidophos, so its use is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] Examples of the present invention are described below.

[0015] Determination of degradation characteristics of degrading bacteria S113 in soil: Weigh 1000g of soil, add metsulfuron-methyl, thifensulfuron-methyl, bensulfuron-methyl, and ethametsulfuron-methyl drug solution, so that the drug concentration in the soil is 10mg / kg, add S113 bacterial solution , mixed evenly, placed in a 30°C incubator for constant temperature cultivation in the dark, and regularly took samples to determine the residual amount of metsulfuron-methyl in the soil. The results are shown in Table 1.

[0016] substrate

metsulfuron-methyl

Thifensulfuron-methyl

Bensulfuron-methyl

ethametsulfuron

Degradation rate%

85.92

88.47

86.12

86.15

[0017] Activate the original seed of the herbicide and pesticide residue-degrading bacteria S113 of the present invention on a petri dish, measure its degradation performance, and inoculate it on the i...

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Abstract

The invention provides a degradation bacillus which can dispel the metoxuron pesticide residue and its prodegradant. The used strain is Gram's stain reaction hysteroptosis s113 in the field of Methylopilus sp. The main biology characteristic is G-; the thaliana is the rod type whose size is 2.2*0.45mu m; the scavenger enzyme, oxidative enzyme and the nitrate reduction reaction are positive; the V.P. reaction and the indole reaction are negative; it can not be hydrolysed starched; the Genbank landing number of the strain 16S rDNA is DQ095877.

Description

1. Technical field [0001] The invention relates to a metsulfuron-methyl pesticide residue degrading bacterium and a bacterial agent produced therefrom, which belong to the high-tech field of biology, use microorganisms to degrade chemical pesticide residues, and are suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Technical background [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by weed damage and other reasons for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production, playing an important role in the modernization of agricultural production. an immeasurable effect. But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to huma...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/00
Inventor 李顺鹏黄星孙纪全何健洪青
Owner NANJING AGRICULTURAL UNIVERSITY
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