51 results about "Stain reaction" patented technology

A bacterial strain for eliminating organophosphorous residue is Granis staining reaction negative bacteria DLL-1 identified as pseudomonas putida. Its biological feature is G- with thallus being short bar shape, tail end being oval and single end being grown thickly with flagellum. The Genbank landing number of 16S rDNA for the bacterial strian is AF 447394.

The invention discloses rhodococcus ruber YMHL-1 capable of degrading nicosulfuron and applications thereof. The invention provides a strain namely rhodococcus ruber YMHL-1 capable of degrading residual nicosulfuron in wastewater, and the identification shows that the strain belongs to Rhodococcus. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC in February 9th, 2015; and the preservation number is CGMCC No. 10542. The main biological characteristics are as follows: the Gram staining reaction is negative; the bacterial colony is in an orange yellow color, is in a round shape, and is opaque, the surface of the bacterial colony is rough and dry; the cell is in a sphere shape or short bar shape, does not have any flagellum, is motionless, and does not generate any spore; the strain is aerobic and chemoheterotrophic and cannot liquefy gelatin and hydrolyze starch; the enzyme contact reaction is positive, the V.P. reaction is positive, the methyl red reaction is negative; the strain cannot degrade casein, cannot reduce nitrates; and the strain can produce acids from glucose, can utilize glucose, fructose, and citrates, cannot utilize mannose, arabinose, and raffinose, and can reduce more than 90% of antibiotics in water through direct application.

The invention provides a pesticide-degrading bacterium for removing the residue of a herbicide of chlorotoluron; the bacterium that is used is gram staining reaction negative bacilli YBL3 which is identified to be Sphingobium sp. The bacterium is preserved in China Center for Type Culture Collection in May 23rd, 2008 and the preservation number of the bacterium is CCTCC M208076. The technique for producing the bacterium includes: slant seed-shake flask seed-seed pot-production pot-product (the packaging formulation is liquid bacterium or solid absorption bacterium). The product of the degrading bacterium can be directly applied to reduce the pesticide residue in the crops by more than 90 percent, thus solving the over standard problem of the pesticide residue in agricultural production and being capable of producing green agricultural products with no poison and social effects of pollution.

The invention provides a prodegradant which can dispel the organic phosphorus pesticide residue of chlorpyrifos. The used strain is Gram's stain reaction hysteroptosis Dsp-2 which is Sphingomonas sp. The main biology characteristic is G-; the thaliana is the rod type of asporous whose size is 1.0-1.5um; the scavenger enzyme is male and the oxidative enzyme is female which can not be hydrolysed starched and be gelatin liquidated; the methyl red reaction is female and not to produce the indole with the aquolysis temperature 80 deg. The Genbank landing number of the strain 16S rDNA is AY994060.

The invention provides dimethylformamide (DMF) degrading bacteria and a bacterial agent produced from the same, which belong to the field of biological high technology. The strain is a Gram staining reaction negative bacteria strain DMF3 and is identified as pseudomonasplecoglossicida. The strain has the main biological characteristic of G<->, an irregular rod-shaped strain body, the size of about 0.5-1.0*1.5-5.0 mu m and no stipe or mycoclena, does not produce spore, is aerobic, undergoes strict respiratory metabolism and can grow by taking DMF as a unique carbon source and nitrogen source so as to be completely mineralized for releasing ammonia nitrogen. The strain can reduce the residual quantity of the DMF with certain concentration in waste water by over 95 percent at the temperature of 30 DEG C under the pH value of 7.0 within 3 days, so that the problem of difficult treatment of the DMF in chemical production waste water is solved, the chemical oxygen demand (COD) of the DMF waste water reaches the standard and the waste water can be discharged.

The invention provides a degrading bacterium eliminating the pollution of triethylamine and a degrading microbial agent and belongs to the high-technical field of biology. The used strain is a gram staining reaction positive bacterium R4, is determined as arthrobacter protophormiae, and has a biological characteristic of G+. The logarithmic phase thallus is bacilliform, sporeless and aerobic to grow, and can grow by taking the triethylamine as the only carbon source or nitrogen source and thoroughly mineralize the triethylamine to generate ammonia nitrogen. In a flask experiment in a laboratory, the strain can completely degrade 100mg / L triethylamine and solve the problem of the difficult biodegradation of the triethylamine in wastewater treatment.

The invention provides a degradation strain for eliminating residual bromoxynil, belonging to the field of biological high technology. The adopted bacterial strain is a gram staining reaction negative strain MXX-04 which is identified to be paracoccussp. The main biological properties are G-, short rod shape, no spores, no motility and positive oxidase and catalase. The strain provided by the invention can grow by taking bromoxynil as a unique nitrogen source and mineralize the bromoxynil. Under the condition of a shake flask in a laboratory, the strain can degrade 95.3% of 100mg / L bromoxynil within 24 hours, thus the problem that bromoxynil is hard to degrade biologically in the environment is solved.

The invention provides degrading bacteria for eliminating residue of pesticide, namely bromoxynil octanoate, and a degrading bacterial agent thereof, and belongs to the technical field of biology high-technology. The strain is Gram staining reaction negative bacteria XB2 and is identified as Acinetobacter baumannii. The strain has main biological characteristics that: the strain is G-; the thallus has a short-rod shape with the size of 0.9-1.6*1.5-2.5 mu m, has no spores and is strictly aerobic; and the strain is negative for oxidase and positive for catalase and can grow by taking the bromoxynil octanoate as a unique carbon source. The degrading bacteria is directly applied to make bromoxynil octanoate residue in crops reduced by over 90 percent, solves the problem that the bromoxynil octanoate residue in agricultural production is overproof, and can produce non-toxic and nuisanceless green agricultural products.

The invention provides a degradation bacillus which can dispel the long-lasting phosphorus pesticide residue and its producing bacillus. The strain is Gram's stain reaction hysteroptosis m-1 in the field of Paracoccus.sp.; the main biology characteristic is G-; the thaliana is the short rod type which likes oxygen and dislikes sport; the scavenger enzyme and the oxidative enzyme is positive; it can not use citrate and nitrate to reduction the positive; the denitrifying reaction generates the gas.

The invention discloses a halogenated phenol-degrading strain and a microbial agent prepared from the halogenated phenol-degrading strain. The strain is a Gram staining reaction negative strain DCP-2and is Bosea sp. through identification. The strain is preserved in the China Center for Type Culture Collection on December 23, 2019 and has a strain preservation number of CCTCC M20191083. The halogenated phenol-degrading strain DCP-2 can be applied to degradation of 2,4-dichlorophenol (2,4-DCP), o-chlorophenol, p-chlorophenol or m-chlorophenol, and is preferentially applied to degradation of 2,4-DCP in soil. A degradation microbial agent can be used for reducing the residual amount of 2,4-DCP in soil to be 95 percent or more by direct application, and the pollution problem of 2,4-DCP in soil can be solved.

The invention provides a degradation microbial inoculum for eliminating piperazine residue, and belongs to the high technical field of biology. The strain is gram staining reaction negative strain PQ-01, and is identified to be paracoccus sp., wherein the main biological characteristics are gram negative, spherical or club shape, asporous and non-motile. The strain can grow by taking piperazine as the unique carbon source and nitrogen source, and can be completely mineralized. 100mg / L of piperazine can be degraded to a level which cannot be detected in 24 hours by the strain under a shaking condition in the lab, so that the problem that the piperazine is difficult to biodegrade in waste water treatment can be solved.

The invention provides DDT pesticide residue degrading bacterial and its produced bacterial agent. The used strain is gram stain reaction positive bacterial strain DB-1. And it is sphingomonas sp by identifying. Its main bionomics is G-; thallus is short baculiform and its size is 0.28 um-0.54um*0.69um-0.85umum its end has flagellum; concurrently character like oxygen; catalase and oxidase are positive; V.P. and indole reactions are negative; it can not hydrolyze starch, oxidize glucose to produce acid, and make litmus milk acid solidify. The strain 16S rDNA Genbank debarkation number is AY947554. The degrading bacterial product can reduce pesticide residue by over 90%, and produce green farm products.

The present invention discloses a carbonate mineral identification method, which comprises: S1, acquiring the rock flake of a carbonate mineral to be identified; S2, carrying out partial staining on one surface of the rock flake to obtain a partially-stained rock flake; and S3, placing the rock flake under a single polarizing microscope and carrying out staining reaction observation within 15 s after the staining, and judging the rock type of the carbonate mineral according to the staining reaction observation result, wherein the one rock flake surface being subjected to the partial staining is named the stained surface, the area of the whole stained surface is recorded as S, and the area of the stained part is recorded as SA. According to the present invention, the partial staining is creatively performed, and the stained flake is placed under the single polarizing microscope and is subjected to the real-time staining reaction observation within 15 s after the staining, such that the identification accuracy is increased, the non-stained part can be re-identified, and the damage rate of the rock flake can be reduced.

The invention provides tebuconazole pesticide degrading bacteria, a soil bio-remediation fungicide based on the bacteria and application of the soil bio-remediation fungicide and belongs to the technical field of biology. The tebuconazole pesticide degrading bacteria are classified and named serratia marcescens H2, the strain is gram staining reaction negative bacteria and is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO:M 2016478, and the preservation date is 13th September, 2016. The tebuconazole pesticide degrading bacteria are a strain which is separated from soil using tebuconazole for many years through enrichment culture and has high degrading capacity to tebuconazole. According to the strain, tebuconazole serves as a unique carbon source for growth, the thallus activity is improved through tebuconazole induction in a liquid medium, and the degrading capacity to tebuconazole is improved. A preparation method of the fungicide is simple, cost is low, application is convenient, the fungicide can be scattered on the soil, the degrading effect in the soil can reach 88%, and the fungicide is suitable for removing pesticide residues in the soil, and has great significance in protecting ecological environment and food safety.

This invention discloses a DNA fluorescence capillary biologic sensor (DNA-FCBS) and its preparation method, it belongs to biochemical field. DNA-FCBS is made by using crosslinking agent fixing DNA probe in capillary tube, it includes capillary tube, poly lysine and DNA probe. It can realize the classification of DNA and quantitative determination. DNA sample solution with butt is inhaled by DNA-FCBS for hybridization and coloration reaction, and then the butt DNA is quantitative determined in fluorescence device according to the fluorescence strength. Different butt nucleotides can be determined by changing DNA-FCBS inner probe type, multiple probes are fixed in DNA-FCBS to determine multiple butt nucleotides once time. The DNA-FCBS also can be made into all kinds of DNA fluorescence capillary biologic testing cassette. The reproducibility of this invention is good, operation is simple, and trace quick determination can be realized, so gene detection can be popular and practical.

The invention provides degrading bacteria and immobilized cells for eliminating diethylamine pollution, and belongs to the high technical field of biology. A used strain is Gram staining reaction negative bacteria EYA-2, and identified as pseudomonas (Pseudomonas sp.). Main biological characteristics are that the strain EYA-2 is G-, logarithmic growth phase thallus is rod-shaped, a bacterial colony is of a round white bulge, nitrate can be reduced, the oxidase reaction is positive, indole is not produced, spores are not generated, and an aerobic growth is carried out. Diethylamine can be taken as the sole carbon source and nitrogen source for the growth, and is completely mineralized to produce ammonia nitrogen. Under the condition of a laboratory shake flask, the degradation rate of 100mg / L diethylamine by the strain reaches 100%, and the problem that diethylamine is difficultly biodegraded in wastewater treatment is solved.

The invention provides a degrading bactericide for eliminating fluoroglycofen-ethyl residues in wastewater, which belongs to the field of organism high technology. The used bacterial strain is a gram staining reaction negative bacterium FC12 which is identified as bacillus. The main biological property of the gram staining reaction negative bacterium FC12 is G-, the gram staining reaction negative bacterium FC12 is straight rod-shaped, the spore of the gram staining reaction negative bacterium FC12 is elliptical, the peritrichous of the bacillus moves, and the size of the gram staining reaction negative bacterium FC12 is 0.5-2.5*1.2-10 microns; catalase is positive; oxidase is negative; and the gram staining reaction negative bacterium FC12 can be used for hydrolyzing starch and liquefying gelatine. Pesticide residue in water can be reduced by more than 90% by direct application of the degrading bacterium product.

The invention provides a degrading bacterium for eliminating triethylamine pollution and an immobilized bead of the degrading bacterium, belonging to the field of high biological technologies. The adopted strain is a gram staining reaction negative bacterium SYA-1 and authenticated as Pseudomonassp. The degrading bacterium has the main biological property G-, a thallus in a logarithmic phase is rod-shaped, a bacterial colony is a circular light yellow non-transparent gelatin which is shaped like a thin film and can be liquefied when located on an LB (Luria-Bertani) flat, and the bacterium is incapable of hydrolyzing starch, positive in oxidase reaction, negative in methyl red reaction, negative in V.P experiments and free of spores and can grow under an aerobic condition. The triethylamine can be used as a unique carbon source and a unique nitrogen source for growing and can be thoroughly mineralized to produce ammonia nitrogen. The degrading rate of the bacterium to 100mg / L of triethylamine under a laboratory shake flask condition is up to 100%, thus the problem that the triethylamine is difficult to biodegrade in wastewater treatment is solved.

The invention provides a degrading bacterial agent for eliminating fluoroglycofen-ethyl residue in wastewater, and belongs to the high technical field of biology. Used strains are Gram staining reaction negative bacteria YS3, and identified as pseudomonas (Pseudomonas sp.). Main biological characteristics are that the fluoroglycofen-ethyl degradation pseudomonas YS-03 is G-, rod-shaped, and free of spores, and has a polarflagella of which the size is 0.8 to 1.3*0.5 to 0.6mu m; catalase is positive; oxidase is negative; starch cannot be hydrolyzed, gelatin cannot be liquefied, the voges-proskauer test reaction is positive, the methyl red negative reaction is negative, indole is not produced, and Tween-80 is hydrolyzed. The pesticide residue in water can be lowered by more than 90% by direct application of a degrading bacteria product.

The invention discloses a color developing agent for fast detecting the high-whiteness flour wheat seed resource and a use method, and belongs to the technical field of wheat flour whiteness detection. On the basis of using a phenol reagent, the phenol solution concentration is regulated; copper sulfate pentahydrate in certain concentration is added; two kinds of solutions in different concentration are mixed into the color developing agent to soak seeds; through seed coat staining reaction, the high-whiteness flour wheat seed resource is identified. Large-scale instruments with complicated tasks are not needed; meanwhile, the flour whiteness detection is moved to the field from a laboratory; by the method, the seeds can be collected at any time in any place in the field; then, the seeds are put into a small beaker for directly performing staining reaction to detect the high-whiteness flour wheat seed resource.

The invention provides a degrading strain capable of eliminating norfloxacin left in wastewater and belongs to the technical field of high techniques for biology. The strain is gram staining reaction negative bacteria NOR-36 and is identified as acinetobacter calcoaceticus. The acinetobacter calcoaceticus is preserved in CGMCC (China general microbiological culture collection center) in Microbiology Research Institute of China Science Academy in No.3, yard No.1, Beichen western road, Chaoyang district, Beijing, the postcode is 100101, the collection number is CGMCC No.10540, and the strain is identified as acinetobacter calcoaceticus. The main biological character is gram-negative, the bacterial colony is smooth, and the strain is short-rod-shaped and is positive when contacted with oxidase and negative when contacted with nitrate reductase. The degrading product can be directly applied and can reduce the amount of residual norfloxacin in water by more than 90%.

The invention discloses a bromoxynil degrading bacterium and application thereof, and belongs to the field of high biotechnologies. A bacterial strain of the bromoxynil degrading bacterium is a Gram staining reaction negative bacterium BX03, and belongs to Burkholderia bacterial genus (Burkholderia sp.) is identified as Burkholderia sp.. The bromoxynil degrading bacterium is mainly biologically characterized in that the bromoxynil degrading bacterium is Gram-negative, is in the shape of a short spar and is free of spores, and flagella grow at the ends of the bromoxynil degrading bacterium. Bromoxynil can be used as the only carbon source and the only nitrogen source to grow the bacterium, and the bacterium can be mineralized. The bromoxynil degrading bacterium and the application have the advantages that 95% of 100mg / L of the bromoxynil can be degraded by the bacterial strain within 24 hours under a flask shaking condition in a laboratory, and accordingly the problem of difficulty in biologically degrading bromoxynil during waste water treatment can be solved.

The invention provides a pear fruit stone cell specific histochemical localization method, and belongs to the technical field of cell staining. The method comprises the following steps: slicing pear fruits in a vitamin C solution to obtain pear fruit slices; submerging the pear fruit slices in a dyeing reaction solution for 2-5 hours to obtain soaked pear fruit slices, wherein the components of the dyeing reaction solution comprise diaminobenzidine or nitrotetrazole; and decolorizing the soaked pear fruit slices by using a decolorizing agent, and sealing the slices by using a stationary liquid. According to the invention, the method is high in distinguishing degree, and can accurately, conveniently and effectively distinguish the pear pulp parenchyma cells from the stone cells; the safetycoefficient is high, hazardous chemical substances are not involved, and pungent smell is avoided; vitamin C effectively reduces the browning phenomenon of slices and prevents color development colorinterference; and morphological structure characteristics in the stone cell development process can be efficiently observed, and pear varieties with different stone cell contents and sizes can be distinguished.

The invention discloses a fur processing wastewater treatment device, which comprises a grid well, a dyeing water collection well, a dyeing reaction tank, a primary sedimentation tank, a hydrolysis tank, an oxidation tank, a secondary sedimentation tank, a secondary reaction tank, a final sedimentation tank, and a discharge well , a sludge thickening tank, a chrome liquid collection well, a chrome liquid reaction tank and a chrome mud concentration tank, the dyeing water collection well is connected to the dyeing wastewater output end, the chromium liquid water collection well is connected to the chromium-containing wastewater output end, and at the dyeing wastewater output end Grille wells are arranged between the dyeing water collection well, between the chromium waste water output end and the chromium liquid water collection well, the output end of the dyeing water collection well is connected to the input end of the dyeing reaction pool, and the output end of the dyeing reaction pool is connected to The input end of the primary sedimentation tank and the output end of the chromium liquid collection well are connected with the input end of the chromium liquid reaction tank. Through the combination of various treatment processes, the present invention can ensure that the effluent is discharged up to the standard, and the present invention has simple process, convenient operation, thorough treatment, energy saving and low cost.

The invention discloses a specific nucleic acid fluorescent staining reaction solution which comprises the following four components: a semen diluent, an acidizing fluid, a fluorescent dye and a dye diluent. The invention also provides a method for detecting sperm DNA fragmentation by using the specific nucleic acid fluorescent staining reaction liquid. The method comprises the following steps: preparing a fluorescent dye; preparing cells; cracking and dyeing the cells; detecting on a machine; result judgment: determining that the spermatids emitting green fluorescence are normal spermatids according to a photographing result; the cells emitting orange and red fluorescence are fragmented sperm cells. Furthermore, the DNA damage proportion in the sample can be calculated according to the number ratio of the two fluorescent cells. The method provided by the invention has the advantages of few operation steps, good specificity and accurate judgment on sperm DNA fragments.

The invention relates to the technical field of biology, in particular to a DNA ploidy staining solution as well as a preparation method and a staining method thereof, and the prepared DNA staining solution can solve the problems that the background is easy to co-stain, the staining time is relatively long and slices are easy to fade in flaking, so that a reliable histochemical staining technologyis provided for tumor cell nucleus DNA content image determination. Alcohol with the concentration of 95% is used for fixing cell nucleuses, the situation that in the dyeing hydrolysis process of unfixed cell nucleuses, DNA can form small fragments and dissociate out of the cell nucleuses, the DNA measurement accuracy is affected is avoided, aldehyde groups generated before hydrolysis can be sealed through fixation, and false positive products are reduced. Meanwhile, the BS stationary liquid can be used for changing the space structure of DNA molecules, so that the sensitivity to acid hydrolysis is influenced, and the situation that chromatin coloring is relatively light due to improper selection of the stationary liquid or too long tissue fixing time and relatively large difference in strength of dyeing reaction is avoided. Through gradient alcohol dehydration, the DNA staining result is clear, and the background is cleaner.

The invention provides a degrading bacterium for eliminating triethylamine pollution and an immobilized bead of the degrading bacterium, belonging to the field of high biological technologies. The adopted strain is a gram staining reaction negative bacterium SYA-1 and authenticated as Pseudomonassp. The degrading bacterium has the main biological property G-, a thallus in a logarithmic phase is rod-shaped, a bacterial colony is a circular light yellow non-transparent gelatin which is shaped like a thin film and can be liquefied when located on an LB (Luria-Bertani) flat, and the bacterium is incapable of hydrolyzing starch, positive in oxidase reaction, negative in methyl red reaction, negative in V.P experiments and free of spores and can grow under an aerobic condition. The triethylamine can be used as a unique carbon source and a unique nitrogen source for growing and can be thoroughly mineralized to produce ammonia nitrogen. The degrading rate of the bacterium to 100mg / L of triethylamine under a laboratory shake flask condition is up to 100%, thus the problem that the triethylamine is difficult to biodegrade in wastewater treatment is solved.