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Pseudomonas SYA-1 for degrading triethylamine and application of pseudomonas SYA-1

A technology of SYA-1 and Pseudomonas, which is applied in the direction of bacteria, enzymes, immobilized enzymes, etc., can solve the problems of high cost and secondary pollution, achieve low production cost, reduce waste water treatment cost, and have good removal effect Effect

Active Publication Date: 2015-03-25
中持(江苏)环境建设有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Triethylamine is widely present in organic synthesis, pesticides, dyes and other production wastewater. At present, physical and chemical methods are mostly used for the treatment of triethylamine-containing wastewater, which is costly and easy to cause secondary pollution; and bioremediation technology due to microbial It can degrade and transform various organic pollutants and has great potential

Method used

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  • Pseudomonas SYA-1 for degrading triethylamine and application of pseudomonas SYA-1
  • Pseudomonas SYA-1 for degrading triethylamine and application of pseudomonas SYA-1
  • Pseudomonas SYA-1 for degrading triethylamine and application of pseudomonas SYA-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Isolation and identification of triethylamine-degrading strain SYA-1

[0023] Take 1.0ml of triethylamine-enriched bacterial solution, add 9.0ml of sterile water, and mix well to make 10 -1 The rich solution, and then draw 1.0ml prepared 10 -1 The enrichment solution was added to 9.0ml sterile water, mixed thoroughly to make 10 -2 The enrichment solution, and so on, carry out gradient dilution on the enrichment solution. Draw 0.1ml of the diluted solution of each gradient and spread it on the inorganic salt solid medium containing 100mg / L triethylamine. The formula is: each liter contains 1.5 g K 2 HPO 4 , 0.5 g KH 2 PO 4 , 0.2 g MgSO 4 ×7H 2 O, 1.0 g NaCl, 1.0 g (NH 4 ) 2 SO 4 , 20g agar, pH 7.0, cultured at 30°C for 7 days. After 7 days, pick a single colony from the above inorganic salt solid medium, culture in 5ml LB liquid medium for 24 hours, centrifuge at 8000r / min for 2min, pour off the supernatant, add 5ml of sterile Shake well with water,...

Embodiment 2

[0024] Example 2 The method for preparing immobilized pellets from bacterial strain SYA-1

[0025] 1) Use an inoculation loop to pick a small amount of bacterial strains on the slant medium and inoculate them in LB liquid medium at 30°C and 160r / min for shaking culture to the logarithmic phase;

[0026] 2) Centrifuge the above cultured bacterial solution at 8000r / min for 2min, discard the supernatant, add the same volume of sterile water, shake well and centrifuge at 8000r / min for 2min, wash twice in this way, and use the same volume Suspended in sterile water to make a bacterial fluid.

[0027] 3) Mix 1ml of bacterial liquid with 20ml of 4% sodium alginate solution at room temperature, then drop the mixture into 3% CaCl at room temperature with a syringe 2 The solution was cross-linked and calcified at 4°C for 6 hours to obtain calcium alginate gel-immobilized beads, which were washed twice with sterile water and stored at 4°C for later use.

[0028] The LB medium described...

Embodiment 3

[0029] The application method of embodiment 3 immobilized bead in triethylamine degradation

[0030] First, aerate the immobilized pellets in wastewater for 12 hours to activate. During the activation process, the water temperature is 28±2°C, the aeration rate is 1.2L / min, and then the temperature is 28±2°C, and the pH value is 6.8-7.2. Under conditions, under the situation that triethylamine influent concentration is lower than 1000mg / L, every liter of triethylamine waste water takes the immobilized pellet prepared in embodiment 2 of 185ml to process, and simultaneously, hydraulic retention time is 32h, as image 3 Shown, in this case the removal rate of triethylamine is more than 97%, and better removal effect is arranged; And Figure 4The removal of triethylamine for different influent concentrations is shown. The research shows that the removal rate of triethylamine decreases with the increase of influent concentration. When the concentration of triethylamine in influent i...

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Abstract

The invention provides a degrading bacterium for eliminating triethylamine pollution and an immobilized bead of the degrading bacterium, belonging to the field of high biological technologies. The adopted strain is a gram staining reaction negative bacterium SYA-1 and authenticated as Pseudomonassp. The degrading bacterium has the main biological property G-, a thallus in a logarithmic phase is rod-shaped, a bacterial colony is a circular light yellow non-transparent gelatin which is shaped like a thin film and can be liquefied when located on an LB (Luria-Bertani) flat, and the bacterium is incapable of hydrolyzing starch, positive in oxidase reaction, negative in methyl red reaction, negative in V.P experiments and free of spores and can grow under an aerobic condition. The triethylamine can be used as a unique carbon source and a unique nitrogen source for growing and can be thoroughly mineralized to produce ammonia nitrogen. The degrading rate of the bacterium to 100mg / L of triethylamine under a laboratory shake flask condition is up to 100%, thus the problem that the triethylamine is difficult to biodegrade in wastewater treatment is solved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a triethylamine-degrading pseudomonas strain SYA-1 and its application. Background technique [0002] Organic amine products are widely used in pesticides, pharmaceuticals, rubber, synthetic dyes, petroleum, leather, detergents, organic synthesis, inorganic chemicals and other industries. There are many types of organic amine products, and each product will have residual raw materials and intermediate products entering the wastewater during the production process. Most of these substances are high in nitrogen and low in carbon, toxic and harmful, and difficult to biodegrade. posed difficulties. [0003] Triethylamine is an important raw material for organic solvents and chemical synthesis. In the manufacture of synthetic resins, it is used as a catalyst for the phosgene process of polycarbonate, a polymerization inhibitor for tetrafluoroethylene and a rubber vulcanization accelerato...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/00C02F3/34C12R1/38C02F101/38
Inventor 顾乔梅张金邱沪生蔡双梅
Owner 中持(江苏)环境建设有限公司
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