Tebuconazole pesticide degrading bacteria, soil bio-remediation fungicide based on bacteria and application of soil bio-remediation fungicide

A technology of soil biology and restoration of bacterial agents, applied in the biological field, can solve problems such as environmental pollution, and achieve the effects of easy use, elimination of pesticide residues, and good degradation effect

Inactive Publication Date: 2017-03-08
JILIN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, it must be accompanied by its residue in the environment during its extensive use, an

Method used

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  • Tebuconazole pesticide degrading bacteria, soil bio-remediation fungicide based on bacteria and application of soil bio-remediation fungicide
  • Tebuconazole pesticide degrading bacteria, soil bio-remediation fungicide based on bacteria and application of soil bio-remediation fungicide
  • Tebuconazole pesticide degrading bacteria, soil bio-remediation fungicide based on bacteria and application of soil bio-remediation fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Enrichment, separation, purification and preparation of bacterial suspension of tebuconazole-degrading bacteria

[0027] Take 5g of soil sample (tebuconazole has been used for many years) and add it to a conical flask filled with 100mL of inorganic salt medium, and take 1mL from the methanol solution of tebuconazole with a concentration of 10000mg / L and directly add it to the conical flask as a microorganism The only carbon source for growth (100mg / L), cultured on a shaker for 7d (30°C, 150r / min) and then transferred to an inorganic salt medium containing tebuconazole 200mg / L, cultured for 7d, and then transferred in turn To culture in the inorganic salt medium containing 300mg / L, 500mg / L, 800mg / L and 900mg / L of tebuconazole, after culturing for 7 days at each drug concentration, then in the plate medium containing 400mg / L of tebuconazole Insert 0.1mL of the above-mentioned culture medium on top, culture in a 30°C incubator for 2 days after coating, pick out ...

Embodiment 2

[0030] Embodiment 2: degradation condition

[0031]1. The effect of pH on the degradation of tebuconazole by strain H2

[0032] It is 4% inoculum amount by volume percentage, the H2 bacterium suspension prepared by embodiment 1 is inserted in the inorganic salt medium that tebuconazole concentration is 200mg / L, regulates the pH of culture medium with hydrochloric acid and sodium hydroxide solution respectively The values ​​were 3, 5, 7, 9, and 11, cultured on a shaker at 30°C and 150 rpm, and the content of tebuconazole was measured at 1, 2, 4, 6, and 8 days, respectively.

[0033] Described inorganic salt medium formula is: K 2 HPO 4 1.5g, KH 2 PO 4 1g,NH 4 NO 3 1g, MgSO 4 ·7H 2 O0.3g, CaCl 2 2H 2 O 25mg, Fe 2 (SO4) 3 8mg, pH natural, 1000mL distilled water.

[0034] The experimental results of pH on the degradation of tebuconazole by strains showed that when the pH was 7.0, the degradation rate reached 91.25% at 6 days, and the degradation efficiency of tebu...

Embodiment 3

[0050] Embodiment 3: the preparation of soil bioremediation bacterial agent

[0051] In the slant test tube strain of tebuconazole-degrading bacteria H2, use an inoculation loop to inoculate a little bacteria on the plate medium, culture at 30°C for 48 hours, then use an inoculation loop to inoculate a little bacteria in LB medium, 30°C Shake culture to the logarithmic phase, the formula of the plate medium is: NaCl 10g, peptone 10g, beef extract 5g, agar 15g, add 1000mL distilled water; the LB medium is: NaCl 10g, yeast extract Powder 5g, tryptone 10g, add 1000mL of distilled water; put 10mL of the cultured strain into the seed bottle, shake culture to the logarithmic phase; 1000mL seed bottle medium is: sucrose 2g, K 2 HPO 4 1.5g, KH 2 PO 4 1g, MgSO 4 ·7H 2 O 0.3g, NH 4 NO 3 1g, CaCl 2 2H 2 O 25mg, Fe 2 (SO4) 3 8mg, pH 7.0, 1000mL distilled water.

[0052] Add 15mL of the cultured strains above to 1000g of fermentation medium consisting of wheat bran, polyvin...

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Abstract

The invention provides tebuconazole pesticide degrading bacteria, a soil bio-remediation fungicide based on the bacteria and application of the soil bio-remediation fungicide and belongs to the technical field of biology. The tebuconazole pesticide degrading bacteria are classified and named serratia marcescens H2, the strain is gram staining reaction negative bacteria and is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO:M 2016478, and the preservation date is 13th September, 2016. The tebuconazole pesticide degrading bacteria are a strain which is separated from soil using tebuconazole for many years through enrichment culture and has high degrading capacity to tebuconazole. According to the strain, tebuconazole serves as a unique carbon source for growth, the thallus activity is improved through tebuconazole induction in a liquid medium, and the degrading capacity to tebuconazole is improved. A preparation method of the fungicide is simple, cost is low, application is convenient, the fungicide can be scattered on the soil, the degrading effect in the soil can reach 88%, and the fungicide is suitable for removing pesticide residues in the soil, and has great significance in protecting ecological environment and food safety.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a tebuconazole pesticide-degrading bacterium and a soil bioremediation bacterium based on the bacterium and its application. It uses microbial technology to degrade chemical pesticide residues, and is suitable for green and non-toxic green in modern agricultural production. Production and processing of polluted agricultural products. Background technique [0002] Tebuconazole is a hydroxyethyl triazole derivative, which is a new generation of high-efficiency broad-spectrum systemic fungicide first developed by Bayer in 1986. It is used in the seeds of important economic crops A high-efficiency, low-toxicity, systemic fungicide for treatment or foliar spraying, which can effectively control various rusts, root rots, scabs, sheath blights, rice false smut and various smuts of cereal crops, etc. . [0003] However, it must be accompanied by its residue in the envir...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A62D3/02C12R1/425A62D101/04A62D101/26A62D101/28
CPCA62D3/02A62D2101/04A62D2101/26A62D2101/28B09C1/10C12N1/205C12R2001/425
Inventor 张浩侯晓娟梁爽逯忠斌王岩侯志广赵晓峰王鑫宏
Owner JILIN AGRICULTURAL UNIV
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