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Recombinant gene engineered strain of aujeszkys disease-porcine reproductive and respirator syndrome virus and its uses

A technology for recombining pseudorabies and respiratory syndrome, applied in the direction of virus antigen components, virus/bacteriophage, medical preparations containing active ingredients, etc., can solve the problem of no biological experiment data, difficult promotion and application, and incomprehensible effects To achieve good prevention and treatment effects, good immunogenicity, and strong protective effects

Active Publication Date: 2006-07-12
WUHAN KEQIAN BIOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The purpose of the invention is to use the strain to prepare a bivalent or multivalent genetically engineered live vector vaccine, but judging from the published specification and claims, the application does not provide any relevant biological experimental data to illustrate the effect of the invention, and cannot It makes people understand the effect of its implementation, and there is no detailed information on how to use the invention and reproduce the effect of the invention in the embodiment, so it is difficult to popularize and apply

Method used

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  • Recombinant gene engineered strain of aujeszkys disease-porcine reproductive and respirator syndrome virus and its uses
  • Recombinant gene engineered strain of aujeszkys disease-porcine reproductive and respirator syndrome virus and its uses
  • Recombinant gene engineered strain of aujeszkys disease-porcine reproductive and respirator syndrome virus and its uses

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Embodiment Construction

[0041] Below in conjunction with accompanying drawing, the present invention will be further described

[0042] 1. Primer design (for gene cloning and molecular detection)

[0043] According to the published ORF5M and ORF6 gene sequences of porcine reproductive and respiratory syndrome virus (Jiang Yunbo et al., Chinese Journal of Veterinary Medicine, 2005 (1), 1-3) design primers, the amplified fragment size is about 650bp and 540bp respectively, and its Restriction sites for BamH I, Xba I, EcoR I, and SmaI were designed at both ends, respectively. At the same time, ORF7 gene sequence primers were designed, and the size of the amplified fragment was about 430bp. Pcmv and PpolyA primers amplify the human cytomegalovirus CMV promoter, MCS multiple cloning site and polyA signal fragment in the pCI-neo (Promega company product) vector, the size is about 1450bp. The above primers were synthesized by Shanghai Sangon Bioengineering Company. The primer sequences are as follows:

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Abstract

The invention relates to a construct, vaccination preparation and application of recombination infectious bulbar paralysis-pig breeding and breathing complex gene project toxic strain in the field of animal virus gene engineering technology. The recombination infectious bulbar paralysis-pig breeding and breathing complex gene project toxic strain is Pseudorabies virus HzauAVL-PRprrsvV-GP5mM which is preserved in CCTCC with the number: CCTCC-V200509. The gene project toxic strain can be used to prepare for infectious bulbar paralysis-pig breeding and breathing complex gene project vaccine.

Description

technical field [0001] The invention belongs to the technical field of animal virus genetic engineering. It specifically relates to a recombinant pseudorabies-porcine reproductive and respiratory syndrome genetically engineered virus strain, a genetically engineered vaccine prepared by using the genetically engineered virus strain and its application. Background technique [0002] Pseudorabies is an acute infectious disease caused by pseudorabies virus (Pseudorabies virus, referred to as PrV) infecting pigs, cattle, sheep, dogs, cats, rabbits, mice, minks, foxes and other domestic and wild animals. Reservoirs and disseminators of viruses (Mettenleiter et al., Comp Immunol Microbiol Infect Dis. 1991, 14: 151-163). Other animals except pigs are in sporadic form, and usually have fever symptoms such as fever, itching, and encephalomyelitis after the onset, all of which are fatal infections. The disease is an outbreak in pigs, and its harm mainly lead...

Claims

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Application Information

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IPC IPC(8): C12N7/01A61K39/12A61K39/295
Inventor 方六荣江云波陈焕春肖少波金梅林吴斌刘正飞
Owner WUHAN KEQIAN BIOLOGY CO LTD
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