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Big Symphyllia mucilage collection method

A collection method, coral technology, applied in animal husbandry, etc., can solve problems affecting research, inability to collect mucus, insufficient mucus, etc., and achieve the effect of easy extraction

Inactive Publication Date: 2006-07-19
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although mucus secretion can be enhanced by environmental stresses such as sediment, fresh water, air exposure, etc., it is not sufficient for certain studies that require large amounts of mucus
Although the low temperature and the method of adding chemical reagents adopted by Meikle et al. (1988) obtain a large amount of mucus, the low temperature and chemical reagents cause damage to stony corals (listed as protected animals), so that the mucus cannot survive after being collected; moreover, chemical reagents can influence subsequent research
[0005] For S. gigantea, it is of great significance to study the compounds in its mucus in terms of chemical ecology and application, but referring to the previous method (without adding chemical reagents), it is impossible to collect a large amount of mucus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] From the fringing reef in the sea area under the jurisdiction of Hainan Tropical Marine Biological Experiment Station (Sanya Luhuitou, Hainan), Chinese Academy of Sciences 2 (cover the reef block surface with oxford paper and measure the area of ​​the paper to get the surface area of ​​the coral block). Carry the pried coral blocks to the shore, and knock twice on the top with an external force of 3kg (with an interval of less than 10 seconds between each time). The corals were placed in a plastic tank filled with seawater and soaked for 60 minutes. The corals were removed and placed upside down in a plastic pot pre-passivated with 0.02% Tween 20 to collect the mucus. The amount of mucus collected reaches 65ml.

Embodiment 2

[0023] From the fringing reef in the sea area under the jurisdiction of Hainan Tropical Marine Biological Experiment Station (Sanya Luhuitou, Hainan), Chinese Academy of Sciences 2 (cover the reef block surface with oxford paper and measure the area of ​​the paper to get the surface area of ​​the coral block). The pried coral block was transported to the shore, and the top of it was tapped three times with an external force of 2.5kg (with an interval of less than 10 seconds between each time). The corals were placed in a plastic tank filled with seawater and soaked for 70 minutes. The corals were removed and placed upside down in a plastic basin pre-passivated with 0.02% Tween 20 to collect the mucus. The amount of mucus collected reaches 100ml.

Embodiment 3

[0025] From the fringing reef in the waters under the jurisdiction of Hainan Tropical Marine Biological Experiment Station (Sanya Luhuitou, Hainan), Chinese Academy of Sciences 2 (cover the reef block surface with oxford paper and measure the area of ​​the paper to get the surface area of ​​the coral block). Carry the pried coral blocks to the shore, and knock twice on the top with an external force of 3.5kg (with an interval of less than 10 seconds between each time). The corals were placed in a plastic tank filled with seawater and soaked for 80 minutes. The corals were removed and placed upside down in a plastic pot pre-passivated with 0.02% Tween 20 to collect the mucus. The amount of mucus collected reached 110ml.

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PUM

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Abstract

The invention provides a method for collecting mucous from giant S.gigantea, which comprises the following steps: (1) prying out a block of healthy giant S. gigantean of moderate size from seabed; (2) knocking the top of pried out coral with extra force for more than twice; (3) immersing in sea for 50-100 minutes; (4) taking out the coral and putting into collecting basin to collect the mucous; (5) pouring the mucous into plastic centrifuge tube, maintaining under temperature of minus 20 Deg. C. The invention collects the mucous from giant S. gigantean by means of physical stimulation, which avoids great damage to coral and guarantee the survival of coral after returning to sea, and the nonuse of chemical agent makes little influence to further research.

Description

technical field [0001] The present invention relates to a method for harvesting the mucilage of the giant synaptic coral for use and research. Background technique [0002] Coral reef ecosystems are among the most productive and diverse on the planet, but in recent decades, many scientists have invested in the disease resistance of stony corals due to the death of coral reefs due to disease outbreaks. under study. The mucus secreted by coral polyps covers the surface of reef blocks formed by coral polyps. This layer of mucus is the first gateway for corals to resist pathogens. Therefore, it is very important to study the anti-bacterial effect of mucus. The compounds contained in mucus can not only be used as anti-infective substances of coral itself, but also the biological activities of many compounds can provide antibiotics, anti-tumor agents and other drugs for humans. [0003] Although the research on mucus of stony corals is of great significance, the difficulty of mu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/00
Inventor 陈国华黄良民谭烨辉
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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