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Hydrophilic and phydrophobic mode sheet base special for biological chip

A biochip, hydrophilic and hydrophobic technology, applied in biological testing, microbial determination/inspection, biochemical equipment and methods, etc., can solve problems such as large spot area, cross-contamination of sample points, high array points, etc., to avoid cross-contamination , Reduce the difficulty of production, the effect of high area consistency

Inactive Publication Date: 2010-04-21
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the contact mode, a hydrophilic film base is usually selected, which is beneficial to transfer the sample to the film base during the contact process, but the hydrophilic film base has the following unavoidable shortcomings in use: 1. It leads to sample spot diffusion As a result, the spot area is relatively large, and high-density array points cannot be obtained, and cross-contamination between sample points is easily caused; 2. The quality of the sample point (reproducibility of sample point volume and area) is largely dependent on the sample distributor.

Method used

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  • Hydrophilic and phydrophobic mode sheet base special for biological chip
  • Hydrophilic and phydrophobic mode sheet base special for biological chip
  • Hydrophilic and phydrophobic mode sheet base special for biological chip

Examples

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Embodiment 1

[0018] see figure 1 , the embodiment of the present invention is provided with a rectangular substrate 1, the upper surface of the substrate 1 is provided with a hydrophobic layer 2, and an array of hydrophilic dots 3 is arranged on the hydrophobic layer 2, and the diameter of the hydrophilic dots 3 is 10 to 500 μm. The center distance between the water dots 3 is 50-1000 μm, the substrate 1 is selected from glass slides or silicon wafers, etc., the hydrophobic layer is selected from polydimethylsiloxane (PDMS), the thickness is 2-5 μm, and the hydrophilic dot film The material of the layer is made of hydrophilic metal (iron, aluminum), and the thickness is 1-3 μm. The contact angle of the hydrophilic surface in the prepared film base is 56°, and the contact angle of the hydrophobic surface is 110°.

[0019] A kind of preparation method of the special-purpose hydrophilic-hydrophobic mode sheet base of biochip of the present invention is given below (there can be many ways to c...

Embodiment 2

[0032] Similar to Example 1, the difference is that a circular base is used, the thickness of the hydrophobic layer provided on the upper surface of the base is 3-5 μm, and an array of hydrophilic square points is arranged on the hydrophobic layer, and the length and width of the hydrophilic square points are equal. is 100 μm, and the center-to-center distance between hydrophilic square points is 800 μn. The substrate is a slide glass with a size of 2.5cm×7.5cm, and the material of the film layer of the hydrophilic square dots is aluminum and hydrophilic metal.

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Abstract

This invention relates to a special hydrophilic and drain mode film base for biochips by providing a special hydrophilic and drain mode film base of a biochip having hydrophilic points in the same area and same size in a modified drain surface (thickness of the drain layer is 2-5mum) on a glass slide, silicon plate or other carriers, in which, shape, area, density and array arrangement can be decided according to the needs and the shape of the points are generally round, the film base has a substrate having a drain layer on the top surface and array hydrophilic points are distributed on the drain layer.

Description

technical field [0001] The invention relates to a biochip substrate, in particular to a modified hydrophobic surface to construct hydrophilic dots with uniform area and size (the shape, area, density and arrangement of the dots can be determined according to actual needs) The biochip is dedicated to the hydrophilic and hydrophobic mode substrate. Background technique [0002] To make a biochip, in addition to special instruments, it is also necessary to choose a suitable solid support--a substrate, that is, a carrier material. As a chip substrate, the following requirements must be met: 1. There are active groups on the surface, which can be coupled with biomolecules; 2. Inertness (does not affect the function of biomolecules) and stability (including mechanical, chemical, physical, etc.); 3. , Good biocompatibility. Generally speaking, all kinds of chip bases choose correspondingly treated silicon wafers, glass wafers, ceramic wafers or polypropylene membranes, nitrocellu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/48C12Q1/00
Inventor 林华水田昭武周勇亮李佳张润香胡维玲
Owner XIAMEN UNIV