Agrobacterium rhizogenes mediated aconitum coreanum and its obtaining method

A technology of Aconitum chrysalis and hair, which is applied in the field of Aconitum chrysantha root and its acquisition, can solve the problems affecting the output and quality of Aconitum chrysalis, not conforming to land resources, long growth cycle, etc., and achieve broad application prospects and utilization value, To overcome the effects of slow reproduction speed and short production cycle

Inactive Publication Date: 2006-12-27
李昌禹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, natural Aconitum japonica is often affected by climate, strong seasonality, and pests and diseases will seriously affect the yield and quality of Aconitum japonica. The growth cycle of Aconitum japonica is long, and it takes 5 years to harvest once, which is affected by climate and pests. Large, unstable qual

Method used

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  • Agrobacterium rhizogenes mediated aconitum coreanum and its obtaining method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1, the cultivation and identification of the root of Aconitum japonica

[0016] 1. Cultivation of the roots of Aconitum japonica

[0017] 1. Materials

[0018] Fresh, healthy leaves of Aconitum japonica in pot, Agrobacterium rhizogenes A 4 .

[0019] 2. Conversion

[0020] 1) Obtaining of explants: Take fresh and healthy leaves of potted Aconitum chrysanthemum, wash them; sterilize them with 0.1% mercuric chloride for 10 minutes, rinse them with sterile water for 4 times, each time for no less than 1 minute, and then blot the surface dry Water; then cut the leaves into 0.5cm×1.0cm size on the ultra-clean bench, transfer to sterilized 1 / 2MS solid medium (5 pieces per bottle); pre-cultivate in the dark for 3 days.

[0021] 2) Cultivation of Agrobacterium rhizogenes: Pick a single colony of Agrobacterium rhizogenes, inoculate it in 5mL liquid YEB, culture it with shaking at 28°C in the dark overnight, inoculate it into 50ml liquid YEB for secondary culture f...

Embodiment 2

[0040] Embodiment 2, the cultivation of Aconitum japonica root

[0041] Take fresh and healthy stems of potted Aconitum chrysanthemum, wash them; sterilize them with 0.1% mercuric chloride for 10 minutes, rinse them with sterile water for 4 times, each time for no less than 1 minute, then blot the surface moisture; then put them on the ultra-clean bench The leaves were cut into 0.5cm×1.0cm size, transferred to sterilized 1 / 2MS solid medium (5 pieces per bottle); pre-cultivated in the dark for 6 days.

[0042] Pick a single colony of Agrobacterium rhizogenes 15834, inoculate it in 5 mL of liquid YEB, culture it overnight with shaking at 28°C in the dark, and inoculate it into 50 mL of liquid YEB for secondary culture for 4-6 hours the next day before use.

[0043] Immerse the pre-cultured Aconitum chrysogenum stem in the cultured Agrobacterium rhizogenes bacterial liquid, infect it for 20min, then use sterile filter paper to suck the bacterial liquid, and then inoculate it on 1...

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Abstract

The invention discloses a yellow aconite hair root and its acquisition method. The yellow aconite hair root provided by this invention is obtained by selecting yellow aconitic organ as explant materials, performing transfection by hair root agricillin and culturing the hair root. The yellow aconite hair root obtained by this invention can reproduce by fluid culture method, the reproductive speed is quick, the period of production is short (about 40 days or so), and it can not be influenced by the natural condition, which can carry out the intensifited industrial production and can overcome the defects that the speed of reprothropology is slow, the growing cycle is long, the output is low and the hereditary stability is weak in artificial planting of yellow aconite hair root; the medicinal components in hair root-the guanfu base A is of high content, which has wide application foreground and utilization value.

Description

technical field [0001] The invention relates to a root of Aconitum chrysalis and its obtaining method. Background technique [0002] Guanfujiasu is a special drug for treating angina pectoris, and it has a very good curative effect with a very small amount. At present, in China, Guanfu A is mainly extracted from the root of Aconitum Coreanum (Levl.) Rapaics (Guanbaifu) for medicinal purposes. However, natural Aconitum japonica is often affected by climate, strong seasonality, and pests and diseases will seriously affect the yield and quality of Aconitum japonica. The growth cycle of Aconitum japonica is long, and it takes 5 years to harvest once, which is affected by climate and pests. Large, unstable quality; and, its root head is small, low per unit yield, which seriously restricts the application of Aconitum chrysanthemum in production. Expanding the planting of Aconitum chrysalis will inevitably compete with grain planting for land, which is not in line with the curren...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12N1/20C12N15/87A01G7/00A01H4/00C12N15/82
Inventor 李昌禹王英平杨福合卢淑波臧埔孙长伟孔祥义
Owner 李昌禹
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