Antineoplastic medicine preparation and prepn. method therefor
A composition and anti-tumor technology, which is applied in the direction of drug combination, bacteria, nervous system diseases, etc., can solve the problems of non-specific anti-tumor effect, neurotoxicity and allergic reaction, and limit anti-tumor effect, so as to achieve easy purchase and high cost. curative effect, less damage effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0036] Example 1 Establishment of Recipient Animals and Tumor Models
[0037] 1. Materials and methods
[0038] 1.1 Bacteria, cells and their culture conditions
[0039] Escherichia coli DH5α, BL21 and HB101 were stably integrated with fluorescent plasmid pXen-1, which were all purchased from Xenogen. Plasmid pXen-1 is resistant to ampicillin, and the bacteria can grow in the medium with ampicillin without adding additional substances. In the experiment of observing the growth inhibition of simple Escherichia coli on tumors, Escherichia coli was cultured in a culture medium with ampicillin at 37°C and 220 rpm for 6-8 hours until the OD600 reached 0.6-0.8. Bacillus growth reached mid-log phase. Bacteria were harvested, washed 3 times with sterile normal saline, centrifuged to precipitate, and finally resuspended with a certain volume of normal saline. The cisplatin used was purchased from the 307 Hospital of the Chinese People's Liberation Army. The cells used were B16 mela...
Embodiment 2
[0045] Example 2 Detection of Escherichia coli enrichment of cisplatin by ICP-MS
[0046] 1. Material: same as embodiment one.
[0047] 2. Method results:
[0048] Shake Escherichia coli DH5α in 5ml LB medium with ampicillin to 0.6-0.8, add 0.5mg, 0.25mg, 0.05mg of cisplatin respectively, and incubate for 1h, 12h, 48h for long-term culture. The Escherichia coli treated with cisplatin were washed five times with 1×PBS, centrifuged, placed in an oven at 60°C for 24 hours, after being thoroughly dried, weighed, and sent to ICP-MS for detection of platinum content. Long-term culture means that the bacteria are transferred to LB medium containing a certain concentration of cisplatin, placed in a shaker, 37°C, 220 rpm, and cultured until the OD of the bacteria reaches about 1.5. It was found that the amount of platinum enriched by Escherichia coli increased with the concentration of cisplatin, and the Escherichia coli absorbed the largest amount of cisplatin after incubation for 2...
Embodiment 3
[0049] Example 3 Observation of the Inhibitory Effect of Simple Bacteria on Tumor Growth
[0050] 1. Materials:
[0051] Same as embodiment one.
[0052] 2. Method results:
[0053] Set up experimental group 1, experimental group 2, and control group. Five mice in each of the experimental group and the control group, Balb / C mice, male, 6-8 weeks old, were purchased from the Experimental Animal Center of the Academy of Military Medical Sciences. Shake the Escherichia coli DH5α transformed with pXen-1 to 0.6-0.8 in the medium with ampicillin, collect the bacteria in the middle logarithmic growth phase, wash with sterile normal saline for 3 times, centrifuge, and reconstitute with a certain volume of normal saline. overhang, the 1 x 10 8 cfu / 100 μl of Escherichia coli was injected into the tumor-bearing mice of the experimental group through the tail vein, and the tumor inoculated into the mice was B16 melanoma, and the injection time was 7 days after the inoculation of the tum...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More