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Application of musculus growth inhibin for preparing anticarcinogen

A technology for muscle growth inhibition and anti-tumor drugs, which is applied in anti-tumor drugs, drug combinations, and pharmaceutical formulations, etc. It can solve the problems of decreased leptin secretion level and fat formation ability, and achieves convenient medication, low cost, and safety high effect

Active Publication Date: 2007-01-31
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Myostatin has the function of inhibiting the differentiation of preadipocytes, and this regulatory process may be related to the signal transduction of C / EBP-alpha and PPAR-gamma; it was also found that the myostatin gene knockout mice showed reduced adipogenesis ability, which eventually led to leptin ( Leptin) secretion level decreased

Method used

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  • Application of musculus growth inhibin for preparing anticarcinogen
  • Application of musculus growth inhibin for preparing anticarcinogen
  • Application of musculus growth inhibin for preparing anticarcinogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, observe the influence of Myostatin on tumor cell growth

[0029]Take the human prostate cancer cell line Du-145, the human cervical cancer cell line Hela and the human lung adenocarcinoma cell line AGZY-83a as examples to observe the effect of Myostatin on the growth of tumor cells. Myostatin lug / mL was added to the culture medium of Du-145, Hela and AGZY-83a cells, and PBS was used as a control, and then continued to culture for 12 hours under the same conditions, and the growth of cells was observed.

[0030] The growth of the above-mentioned cells after the end of the culture is as follows: figure 1 As shown (A.Du-145PBS group, B.Du-145Myostatin group, C.Hela PBS group, D.Hela Myostatin group, E.AGZY-83a PBS group, F.AGZY-83aMyostatin group), after adding Myostatin, paste The walls of Du-145, Hela and AGZY-83a cells gradually shrunk and detached, while the control cells grew normally, indicating that Myostatin has a killing effect on tumor cells.

Embodiment 2

[0031] Example 2, detecting the apoptosis of tumor cells under different concentrations of Myostatin

[0032] Taking the human lung adenocarcinoma cell line A549, AGZY-83a and the human liver cancer cell line HepG2 as examples, the apoptosis of tumor cells under different concentrations of Myostatin was detected. and HepG2 cells were counted by flow cytometry combined with Annexin V-FITC / PI fluorescent staining, and then different concentrations of Myostatin (control, 50, 100, 200, 500, 1000 ng / mL) were added to the culture medium, Continue to culture under the same conditions for 12 hours, count the apoptotic cells with the same method after the end of the culture, and calculate the proportion of apoptotic cells, wherein the cell counting method of flow cytometry combined with Annexin V-FITC / PI fluorescent staining includes the following steps:

[0033] 1) Digestion and collection of cells: Aspirate the culture medium. Since the dead cells have been suspended, transfer the cu...

Embodiment 3

[0036] Example 3, DAPI staining to observe the morphology of apoptotic cell nuclei after Myostatin treatment

[0037] Add Myostatin lug / mL to the culture medium of AGZY-83a, HepG2, A549 and Hela adherently cultured under the same conditions, and then continue to culture under the same conditions for 12 hours. The morphology of the nucleus of apoptotic cells, the staining method is as follows:

[0038] First use the same method as in Example 2 to digest and collect the cells, then fix the cells and stain with DAPI: suspend the cells with 3.7% formaldehyde solution, fix on ice for 5 minutes, centrifuge at 4°C and 3000rpm for 10 minutes; discard the supernatant , the cell pellet was washed with cold PBS, and treated with 0.2% (V / V) Triton at 37°C for 15 minutes; then washed with cold PBS, and added with DAPI staining solution (dissolve DAPI in phosphate buffer, the working concentration was lug / mL), centrifuge at 4°C and 3000rpm for 10 minutes, discard the supernatant; suspend t...

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Abstract

An application of myostatin in preparing the orally taken antineoplastic medicines for treating prostatic cancer, cencer of uterine cervix, lung cancer, liver cancer, etc is disclosed.

Description

technical field [0001] The present invention relates to the new application of cytokines, in particular to the application of Myostatin in the preparation of antitumor drugs. Background technique [0002] The tumor problem is one of the unsolved problems in the scientific community today. Tumor tissue grows rapidly and uncontrollably, can invade or metastasize to any other organs and form new lesions, seriously affecting the normal physiological functions of organs, and overly fast-growing tumors can also consume nutrients and energy to cause cachexia, and are prone to recurrence and difficult to cure . These properties make tumor one of the major killers threatening people's health and life today. Therefore, there is an urgent need for drugs capable of killing tumor cells and curing diseases involving multiple organs and systems caused by tumors. [0003] Apoptosis refers to the process of cell suicide tightly regulated by genes. Many cells continue to grow due to the l...

Claims

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Application Information

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IPC IPC(8): A61K38/19A61P35/00
Inventor 边睿赵相轩刘泳朱大海陈佺
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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