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Method for producing polyhydroxyalkanoate

A technology of polyhydroxyalkanoate and a manufacturing method, which is applied in recombinant DNA technology, fermentation and other directions, can solve the problems of unpractical use of solvent extraction method, time-consuming, low solubility and the like

Inactive Publication Date: 2007-03-07
KANEKA CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is disadvantageous in that it is complicated to operate on an industrial scale, requires an additional large amount of solvent, and increases equipment costs.
[0008] Due to the low solubility of PHA, it is desirable to extract at as high a concentration as possible, but the higher the concentration, the more difficult and time-consuming a series of operations including removal of residue after extraction tends to be, and there is concern that the molecular weight will decrease significantly
However, there is no technique for suppressing the decrease in molecular weight in a series of operations assumed to be practical, so the solvent extraction method, which is considered to be simple, is not actually put into practical use at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The dried cells obtained in Comparative Example 1 were vacuum-dried at 50° C. for 5 hours. The water content of the obtained dried bacterial cells was 4.9% by weight. Using 10 g of the dried bacterial cells, extraction with toluene was performed in the same manner as in Comparative Example 1, and PHBH was recovered. The recovered amount was 5.5 g (92% recovery), and the purity was 99%. The weight average molecular weight was 750,000, which was a sufficient molecular weight for processing, although it decreased by 25% within 1 hour.

Embodiment 2

[0071] The dried cells obtained in Comparative Example 1 were vacuum-dried at 50° C. for 10 hours. The water content of the obtained dried bacterial cells was 2.6% by weight. Using 10 g of the dried bacterial cells, extraction with toluene was performed in the same manner as in Comparative Example 1, and PHBH was recovered. The recovered amount was 5.5 g (92% recovery), and the purity was 99%. The weight-average molecular weight was 800,000, which only decreased by 20% in 1 hour.

Embodiment 3

[0073] The dried cells obtained in Comparative Example 1 were vacuum-dried at 50° C. for 20 hours. The moisture content of the obtained dried bacterial cells was 1.8% by weight. Using 10 g of the dried bacterial cells, extraction with toluene was performed in the same manner as in Comparative Example 1, and PHBH was recovered. The recovered amount was 5.5 g (92% recovery), and the purity was 99%. The weight average molecular weight was 850,000, which only decreased by 15% in 1 hour.

[0074] Examples 1 to 3 show that the decrease in the molecular weight of PHA can be significantly suppressed by reducing the water content in the dried cells.

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Abstract

The present invention provides a method for obtaining a biodegradable polyhydroxyalkanoate by a solvent extraction method without causing a significant molecular weight decrease. The present invention relates to a method for producing a polyhydroxyalkanoate which comprises extracting a polyhydroxyalkanoate from a polyhydroxyalkanoate-containing biomass having the water content of 5% by weight or less using an extraction solvent, crystallizing, and recovering the resultant.

Description

technical field [0001] The present invention relates to a method for producing polyhydroxyalkanoate. Background technique [0002] Polyhydroxyalkanoate (hereinafter abbreviated as PHA) is a biodegradable thermoplastic polyester synthesized and accumulated as an energy storage substance in the cells of many kinds of microorganisms. PHA, which is produced by microorganisms using natural organic acids or oils as carbon sources, can be incorporated into the natural carbon cycle process because it can be completely biodegraded by microorganisms in soil or water. Therefore, PHA is known as an environmentally friendly plastic material with little adverse effect on the ecosystem. In recent years, synthetic plastics have become a serious social problem from the viewpoint of environmental pollution, waste disposal, and petroleum resources. PHA has attracted attention as a green plastic that does not pollute the environment, and its practical application is eagerly expected. [0003]...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12N15/09
CPCC12P7/62C12P7/625
Inventor 木下浩一柳田义文小坂田史雄上田恭义卡鲁纳卡兰·纳拉辛汉安杰拉·C·西尔利肯尼思·伊野田勇夫
Owner KANEKA CORP
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