Method for producing polyhydroxyalkanoate
A technology of polyhydroxyalkanoate and a manufacturing method, which is applied in recombinant DNA technology, fermentation and other directions, can solve the problems of unpractical use of solvent extraction method, time-consuming, low solubility and the like
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Embodiment 1
[0069] The dried cells obtained in Comparative Example 1 were vacuum-dried at 50° C. for 5 hours. The water content of the obtained dried bacterial cells was 4.9% by weight. Using 10 g of the dried bacterial cells, extraction with toluene was performed in the same manner as in Comparative Example 1, and PHBH was recovered. The recovered amount was 5.5 g (92% recovery), and the purity was 99%. The weight average molecular weight was 750,000, which was a sufficient molecular weight for processing, although it decreased by 25% within 1 hour.
Embodiment 2
[0071] The dried cells obtained in Comparative Example 1 were vacuum-dried at 50° C. for 10 hours. The water content of the obtained dried bacterial cells was 2.6% by weight. Using 10 g of the dried bacterial cells, extraction with toluene was performed in the same manner as in Comparative Example 1, and PHBH was recovered. The recovered amount was 5.5 g (92% recovery), and the purity was 99%. The weight-average molecular weight was 800,000, which only decreased by 20% in 1 hour.
Embodiment 3
[0073] The dried cells obtained in Comparative Example 1 were vacuum-dried at 50° C. for 20 hours. The moisture content of the obtained dried bacterial cells was 1.8% by weight. Using 10 g of the dried bacterial cells, extraction with toluene was performed in the same manner as in Comparative Example 1, and PHBH was recovered. The recovered amount was 5.5 g (92% recovery), and the purity was 99%. The weight average molecular weight was 850,000, which only decreased by 15% in 1 hour.
[0074] Examples 1 to 3 show that the decrease in the molecular weight of PHA can be significantly suppressed by reducing the water content in the dried cells.
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