Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Affinity particle and affinity separation method

A separation method and affinity technology, applied in the field of affinity separation, can solve the problems that organic particles are prone to agglomeration, require more time for purification, and have low capture efficiency, and achieve prevention of non-specific adsorption, excellent dispersibility, and low purification efficiency. Effect

Inactive Publication Date: 2007-05-02
SHISEIDO CO LTD
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fatal problems shown in the following (1)-(3) in column separation
[0003] (1) In order to obtain the target substance, various columns must be used, and the purification efficiency is poor
[0004] (2) Confirmation experiments must be carried out to confirm whether the target substance is contained in the separated components, so purification takes more time
[0005] (3) The loss during purification is large, requiring a large amount of samples
[0009] (2) The capture efficiency is low and a large amount of liquid sample is required
[0011] Affinity particles containing organic particles In addition to the above-mentioned fatal problems, there is also the problem that organic particles are prone to agglomeration in samples with high salt concentration

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Affinity particle and affinity separation method
  • Affinity particle and affinity separation method
  • Affinity particle and affinity separation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0228] "Example 1"

[0229] "Affinity Particles"

[0230] The affinity separation method shown in claim 6 is given below. Add 10 mg of the aldehyde body of Synthesis Example 1 to the 2-aminoethyl methacrylate-N-isopropylacrylamide-methylenebisacrylamide particles obtained in 0.1 g of Reference Example 2, stir overnight, then place in an ice-water bath Add 3 mg of sodium cyanoborate to , stir for 6 hours, and then purify and prepare by dialysis in water. To 25 mg of the affinity particles, 1 mL of 8% glutaraldehyde solution and 10 mg of sodium cyanoborohydride (Cianotrihidrohou acid Natrium) for stabilizing the Schiff base were added, reacted at room temperature for 5 hours, and purified by centrifugation with PBS. (5000g) 5 times. Affinity particles according to claim 2 are obtained in which glutaraldehyde is a ligand-binding reactive group or adsorption group. Next, 1 ml of 1 mg / ml bovine albumin or 1 mg / ml human hemoglobin and 10 mg of sodium borohydride were added, reac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The object of the present invention is to provide affinity particles, which are characterized in that: the surface of the organic particles has a phosphorylcholine group represented by the following formula (1) in the form of covalent bonding or adsorption. A ligand with specific affinity for a target substance. Another object of the present invention is to provide an affinity separation method that can easily and accurately separate a target substance by using affinity particles of organic particles.

Description

technical field [0001] The present invention relates to affinity particles and affinity separation methods. More specifically, it relates to affinity particles using organic particles and an affinity separation method capable of separating target substances with high precision and easily. The affinity particles of the present invention are also extremely useful for various separation, purification, and detection methods represented by immunoprecipitation, latex agglutination, and the like, which can detect target substances with high precision and easily. Background technique [0002] Conventionally, separation and purification of biological substances have been performed by column chromatography. However, there are fatal problems shown in the following (1)-(3) in column separation. [0003] (1) In order to obtain the target substance, various columns must be used, and the purification efficiency is poor. [0004] (2) Confirmation experiments must be carried out to confir...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22C07F9/09B01J20/286B01D15/38B01J20/32G01N33/543G01N33/566
CPCB01J20/32Y10T428/2991C07F9/091B01D15/3804B01J20/286G01N33/54393B01J20/3242B01J20/321B01J20/3212B01J20/3219B01J20/3236B01J20/3251B01J20/3274B01J20/3285
Inventor 宫泽和之前野克行
Owner SHISEIDO CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com