Dendritic cell obtained by antigen pulsing

A technology of dendritic cells and dendritic cells, applied in animal cells, virus antigen components, vertebrate cells, etc., can solve problems such as heavy economic burdens, and achieve the effect of improving immune response and immune response

Inactive Publication Date: 2007-05-30
博傲西腾医疗科技(上海)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The frequency of administration must be once every two weeks, which will bring a heavy economic burden of 2.5 million yen a year

Method used

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  • Dendritic cell obtained by antigen pulsing
  • Dendritic cell obtained by antigen pulsing
  • Dendritic cell obtained by antigen pulsing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0172] The experimental examples of the present invention will be specifically described below, but the present invention is not limited to the following experimental examples.

experiment example 1

[0173] (Experimental example 1) Preparation of dendritic cells

(1) Use a heparin (Novo-heparin injection 1000: manufactured by Aventis) syringe (Terumo SyringeSS-50ESZ: manufactured by Terumo) from each subject (five healthy volunteers who have repeatedly used HB vaccine experience as the test subjects) Blood collection 40-60mL. Fully mix the collected blood with the same amount of phosphate buffered saline (PBS) in a clean bench. Then, mononuclear cells were isolated by density gradient centrifugation using Ficoll-Conray solution (specific gravity 1.077). At room temperature, centrifuge at 1800rpm-3000rpm for 30 minutes, and take out the middle layer after centrifugation as mononuclear cells.

[0174] (2) At the same time, autoserum was obtained from each subject according to the following procedure. First, 10 mL of blood was collected from each subject with a heparin-free syringe (Terumo Syringe SS-10ESZ: manufactured by Terumo Corporation). Then, the collected blood wa...

experiment example 2

[0181] (Experimental Example 2) Dendritic cells were pre-excited with HB vaccine.

(1) The dendritic cells obtained in Experimental Example 1 were divided into two. Add HB vaccine (r-HB vaccine "Mitsubishi": manufactured by Mitsubishi Pharma Co., Ltd.) at a final concentration of 2.5-5 μg / mL to 1 aliquot of dendritic cells (ingredients: HBs antigen 20 μg / mL, ethylmercury thiosalicylic acid Sodium 0.01w / v% / mL, Aluminum Potassium Sulfate 5.2g / mL), cultivated for 8-24 hours, thus obtained HB vaccine pre-excited dendritic cells containing HBs antigen. (Hereafter, this cell is referred to as "HB vaccine-primed dendritic cell"). In a dedicated culture room at 37°C, 5% CO 2 Concentration, cultured under the condition of 75% air. Another set of dendritic cells was cultured without HB vaccine as a negative control.

[0182] (2) After culturing, HB vaccine-pre-excited dendritic cells were recovered from the culture dish. PBS was added to the recovered cells, centrifuged at 1500 rpm...

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Abstract

It is intended to provide a dendritic cell efficacious in preventing and treating diseases, mainly hepatitis B. A dendritic cell capable of presenting an HBs antigen, which is obtained by a method involving the step of pulsing a dendritic cell with the HBs antigen, is efficacious in preventing and treating hepatitis B. As the HBs antigen, use can be made of an HBs antigen contained in an HB vaccine. A vaccine-pulsed dendritic cell, which is obtained by a method involving the step of pulsing a dendritic cell with a vaccine, is usable not only for patients but also for healthy subjects. As the vaccine to be used in the pulsing, various types of vaccines can be employed.

Description

technical field [0001] The present invention relates to dendritic cells that can be used for disease prevention or treatment. Background technique [0002] (Dendritic cell therapy and its problems) Dendritic cell therapy in cellular immunotherapy is attracting attention in fields such as cancer treatment. [0003] Dendritic cells are the antigen-presenting cells (antigenpresenting cell: APC) with the strongest antigen-presenting ability in the body. They can present the antigen epitopes they bind to T cells to activate T cells and improve immunity answer. [0004] Dendritic cell therapy refers to a treatment method in which dendritic cells are pre-excited by cancer cell extracts or cancer-specific antigens in vitro to improve the ability of antigen presentation and then reintroduced into the body. [0005] Cancer cell extracts are generally a mixture of various substances. Therefore, dendritic cells pre-excited by cancer cell extracts are considered to be pre-excited by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06C12N5/08A61K35/14A61K39/29A61P1/16A61P31/12A61K35/12A61K35/15A61P31/20A61P37/04A61P43/00C12N5/07C12N5/0784C12N5/0786
CPCA61K2039/5154A61K39/292A61K39/12A61P1/16A61P31/12A61P31/20A61P37/04A61P43/00
Inventor 恩地森一SK.·MD.·法兹勒·阿克巴尔
Owner 博傲西腾医疗科技(上海)有限公司
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