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Pig meat quality character gene FOX03A cloning and its use

A gene and trait technology, applied in the application of molecular markers of pork quality traits, the field of cloning of pork quality traits related gene FOXO3A gene

Inactive Publication Date: 2007-06-13
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In recent years, the research on the economic traits of livestock and poultry using DNA markers has been comprehensively carried out, especially the research on the growth and carcass quality of pigs has made great progress, but the research on the QTL affecting muscle quality by using molecular markers is still relatively limited. few

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  • Pig meat quality character gene FOX03A cloning and its use
  • Pig meat quality character gene FOX03A cloning and its use
  • Pig meat quality character gene FOX03A cloning and its use

Examples

Experimental program
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Effect test

Embodiment 1

[0088] Example 1: Distribution of PCR-RFLP-AvaII polymorphism in various pig breeds

[0089] Six pig breeds were detected by PCR-AvaII-RFLP: among them, the pig breeds belonging to Chinese local blood were Xiaomeishan, Tongcheng pig, Dahuazi pig, Jiangxi Yushan pig, and the pig breeds of foreign origin such as European and American blood were respectively Duroc pigs and large white pigs, these pig breeds basically cover the genotypes of various pig breeds. The gene frequencies of the pig breeds used in the experiment are shown in Table 3. It was found that the frequencies of allele A and allele B in Yushan pigs were close, and allele A was dominant in the other pig breeds.

[0090] Table 3 FOXO3A gene PCR product genotype frequency and gene frequency

[0091] Variety

number of individuals

No. of

genotype

allele frequency

AAA

AB

BB

A

B

large white

Xiaomeishan

Tongcheng pig

Yushan...

Embodiment 2

[0098] Association analysis of pig marker traits

[0099] The association analysis of the AvaII-RFLP polymorphism site in the second exon of the pig FOXO3A gene and some carcass and meat quality traits was carried out. The genotype detection results showed that there were 93 AA genotypes and 108 AB genotypes in 219 individuals. There are 17 individuals with BB genotypes (the actual values ​​obtained are shown in Table 5 due to the lack of data on individual measured traits). In the correlation analysis with some production traits, the analyzed traits include some production traits and meat quality traits , the relevant traits are carcass slant length, 6-7 fat thickness, and drip loss.

[0100] It can be seen from Table 5 that the A allele is a favorable marker for carcass oblique length and 6-7 fat thickness traits (carcass length, thin backfat), and the AA genotype marker can be used as a selection marker for simultaneously increasing body length and reducing backfat thicknes...

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Abstract

This invention belongs to animal genetic engineering technology. Concretely speaking, it involves the pig FOXO3A cloning and its application as molecular markers. The sequence length of meat quality traits gene is 1153bp, encoding 383 amino acids. There is one point mutation in the sequence with T77 changed into C77, resulting the Avall-RFLP polymorphism, which can be used in meat quality traits of correlation analysis. The FOXO3A gene of this invention can be used as a new molecular marker for pig's marker assisted breeding.

Description

technical field [0001] The invention belongs to the technical field of animal genetic engineering, and in particular relates to the cloning of FOXO3A gene, a gene related to pork quality traits, and its application as a molecular marker for pork quality traits. Background technique [0002] In the past few decades of breeding practice, the growth rate, feed remuneration and lean meat percentage of pigs have all been greatly improved. At the same time, due to the negative genetic correlation between meat quality traits and growth traits or carcass traits, the selection of increasing growth rate and lean meat percentage is often accompanied by the decline of meat quality, that is, the deterioration of meat quality and flavor . It has seriously affected pig production and pork processing and consumption, and has attracted the attention of consumers and pig breeders. In order to ascertain the genetic law of meat quality, various studies have been carried out on meat quality at...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/47C12N15/10C12Q1/68
Inventor 赵书红于晶朱猛进李奎刘榜樊斌余梅李长春
Owner HUAZHONG AGRI UNIV
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