Bacillus and production of monodesmosidic panasaponin and aglucon therewith

A technology of bacillus and ginsenosides, applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of occupying land, polluting the environment, etc., and achieve the advantages of reducing emissions, high product recovery rate, and simple and convenient methods. Effect

Inactive Publication Date: 2007-06-20
辽宁新中现代医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of collecting ginseng, the seeds and fruits are collected at the same time, the seeds are used to reproduce offspring, and the pulp is often thrown away as waste, which not only pollutes the environment but also occupies land

Method used

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  • Bacillus and production of monodesmosidic panasaponin and aglucon therewith
  • Bacillus and production of monodesmosidic panasaponin and aglucon therewith
  • Bacillus and production of monodesmosidic panasaponin and aglucon therewith

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Transformation of Panax notoginseng stem and leaf saponins by A8

[0076] Inoculate the A8 cultivated under optimal culture conditions into an aqueous solution containing 10 g of notoginseng stem and leaf saponins (content > 80%). -1 , Conversion (hydrolysis) under natural pH conditions. Sampling starts after 3d. The results of TLC showed that compared with the control group, the spots of C-K, PPD and PPT gradually increased and the color deepened with the prolongation of time, while the content of Rb, Rd and Rg with larger Rf value in the stem and leaf saponins of Panax notoginseng decreased correspondingly . After 13 days of transformation, the reaction product was passed through a 0.2μm-0.45μm microporous membrane to remove microorganisms, and then injected into a D101 macroporous adsorption resin column, washed with water until colorless, and then washed with ethanol gradient to wash the resin column. The 80-90% ethanol eluate was concentrated and evaporated to d...

Embodiment 2

[0078] Transformation of ginseng fruit glycosides by A8

[0079]Inoculate the A8 cultivated under optimal culture conditions into an aqueous solution containing 1 g of ginseng fruit glycosides (content > 60%), at a temperature of 30°C and a shaker rotation speed of 160 r min -1 , Conversion (hydrolysis) under natural pH conditions. After 13 days of transformation, the reaction product was passed through a 0.2 μm microporous membrane to remove microorganisms, then injected into a D101 macroporous adsorption resin column, washed with water until it was colorless, and then washed with an ethanol gradient to wash the resin column. The 80-90% ethanol eluate was concentrated and evaporated to dryness to obtain 0.24 g of a mixture of C-K, PPD and PPT, with a conversion rate of 24%.

Embodiment 3

[0081] Transformation of American Ginsenosides by A8

[0082] Inoculate A8 cultivated under optimal culture conditions into an aqueous solution containing 2g American ginsenoside (content > 90%), transform (hydrolyze under the conditions of 160r min-1 and natural pH value at a temperature of 30°C) ). After 13 days of transformation, the reaction product was passed through a 0.2 μm microporous membrane to remove microorganisms, then injected into a D101 macroporous adsorption resin column, washed with water until it was colorless, and then washed with an ethanol gradient to wash the resin column. The 80-90% ethanol eluate was concentrated and evaporated to dryness to obtain 1.16 g of a mixture of C-K, PPD and PPT, with a conversion rate of 58%.

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PUM

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Abstract

Bacillocin and production of monose-chain ginseng saponin and aglycone are disclosed. The process is carried out by zymolysis acting by bacillocin A8 at 25-45degree and pH value 5.0-7.0 for 2-15hrs, collecting reactant, removing microorganism by 0.2-0.45mum micro-porous filter film, filling it into D101 macro-porous adsorptive resin column, washing by water to be colorless, gradient washing for resin column by alcohol, concentrating for 80-90% alcohol eluent, volatilizing and drying to obtain C-K, PPD and PPT mixture. The converting liquid is aqueous solution and the proportion of ginseng saponin and microbe strain A is 4:1-8:1. Its absorbing capacity reaches to 70-90%.

Description

Technical field: [0001] The invention relates to a method for preparing monosaccharide chain ginsenosides and aglycones by transforming (hydrolyzing) ginsenosides with microorganisms. In particular, it involves a strain of Bacillus sp. A8 [(Bacillus sp.), date of deposit: April 26, 2006, depository unit: General Microorganism Center (CGMCC), China Committee for Microbial Culture Collection, deposit number: 1703, classification name: Bacillus (Bacillus sp.)] transforming ginsenosides to produce monosaccharide chain ginsenoside 20(S) protopanaxadiol-20-O-β-D-glucopyranoside [20(S)-protopanaxadiol-20- O-β-D-glucopyranoside, ginsenoside Compound-K referred to as C-K], protopanaxadiol aglycon [20(S)-protopanaxadiol, referred to as PPD] and protopanaxatriol aglycone [20(S)-protopanaxatriol, referred to as PPT] method. Background technique: [0002] Ginsenoside is the main active ingredient of Araliaceae Panax genus plants such as ginseng, American ginseng, Panax notoginseng, Rh ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12P33/00C12R1/07
Inventor 赵余庆姜彬慧韩颖
Owner 辽宁新中现代医药有限公司
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