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Tissue culture seedling producing process for lechang michelia and golden leaf michelia

A technology of tissue culture and gold leaf, applied in horticultural methods, botany equipment and methods, plant cells, etc., can solve the problems of shortening breeding time, low reproduction coefficient, low natural seed setting rate of seeds, etc., to shorten the breeding time , the effect of high reproduction coefficient

Inactive Publication Date: 2007-06-27
吴月燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The tissue culture of Lechang Michelle and Golden Leaf Michelle is generally propagated by seeds, but due to the low rate of natural seed setting, difficulty in seed collection, low reproduction coefficient and long propagation cycle, it is not conducive to mass reproduction
In addition, because Michelia Lechang and Michelia jinye are perennial woody plants, conventional breeding methods take a long time and have poor results, which greatly shortens the breeding time
Michelia Lechang and Michelia jinye are more difficult to adopt conventional tissue culture. Compared with other plants, they contain more lipids and phenolic substances. It is difficult to occur, especially the root system is not easy to occur, and it cannot be successful with conventional tissue culture methods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Lechang Michelia, Jinye Michelia tissue culture seedling method, including the following steps:

[0027] 1), material selection and disinfection

[0028] When the weather is fine in autumn or spring, pick the young shoots with buds from the 3-year-old branches of Lechang Michelle, put them in the refrigerator at 5°C for 4 days, take them out for disinfection, first rinse them with tap water for 5 hours, and wash them. Soak the essence for 30 minutes, rinse with sterile water 3 times; then sterilize with 70% alcohol for 40 seconds, rinse with sterile water 5 times; finally sterilize with 0.1% mercuric chloride for 10 minutes, wash with sterile water 10 times for inoculation;

[0029] 2), bud induction and proliferation culture

[0030] Inoculate the sterilized buds on MS+6-BA0.8·L-1+IBA0.08·L-1 induction medium, and transfer to the proliferation medium when the adventitious buds germinate to a length of 2 cm; The above materials were subcultured once every 40 days, and...

Embodiment 2

[0037] 1), material selection and disinfection

[0038] When the weather is fine in autumn or spring, pick the young shoots with buds from the 2-year-old branches of Lechang Michelle, put them in the refrigerator at 3°C ​​for 4 days, take them out for disinfection, first rinse them with tap water for 4 hours, and wash them. Soak the essence for 40 minutes, rinse with sterile water 5 times; then sterilize with 70% alcohol for 30 seconds, rinse with sterile water 3 times; finally sterilize with 0.1% mercuric chloride for 10 minutes, wash with sterile water 10 times for inoculation;

[0039] 2), bud induction and proliferation culture

[0040] Inoculate the sterilized buds on MS+6-BA0.8~1.2mg·L-1+IBA0.08~0.12mg·L-1 induction medium, wait for the adventitious buds to germinate to 1~2cm long and transfer to Proliferation medium; the material on the proliferation medium is subcultured once every 30-40 days, and after 12-17 generations of continuous transfer, it is transferred to th...

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Abstract

The tissue culture seedling producing process for Lechang michelia and golden leaf michelia includes the following steps: 1. treating tender michelia stem segment with bud at 3-5deg.C for 4-8 days before conventional sterilizing; 2. inoculating the sterilized bud in inducing culture medium to germinate indefinite bud, shifting to proliferating culture medium after the indefinite bud grows to 1-2 cm long, subculturing for 12-17 generations and shifting into rooting culture medium; 3. dark culturing for 8-12 days before normal rooting culturing; and 4. hardening off under natural lighting after the root grows to 2 cm long for 6-8 days and transplanting. The present invention has the advantages of high propagation coefficient, being suitable for factory seedling raising, high transplanting survival rate, etc.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method for Lechang Michelia and Jinye Michelle. Background technique [0002] The tissue culture of Lechang Michelle and Golden Leaf Michelia is generally propagated by seeds, but due to the low natural seed setting rate, difficulty in seed collection, low reproduction coefficient and long reproduction cycle, it is not conducive to mass reproduction. In addition, because Michelia Lechang and Michelia jinye are perennial woody plants, conventional breeding methods take a long time and have poor results, which greatly shortens the breeding time. Michelia Lechang and Michelia jinye are more difficult to adopt conventional tissue culture. Compared with other plants, they contain more lipids and phenolic substances. Occurrence has a certain degree of difficulty, especially the root system is not easy to occur, and it cannot be successful with conventional tissue cultu...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04A01G1/00
Inventor 吴月燕
Owner 吴月燕
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