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Multiple passage intelligent temperature-control device facing PCR microfluidic chip

A microfluidic chip and chain reaction technology, which is applied in enzymology/microbiology devices, biochemical cleaning devices, bioreactor/fermenter combinations, etc., can solve the problem of low heating efficiency of copper blocks, large system volume, cost advanced questions

Inactive Publication Date: 2007-07-11
BEIJING UNIV OF TECH
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  • Abstract
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Problems solved by technology

The disadvantages of this device are: the heating efficiency of the copper block is low, the whole system is bulky, and it is not conducive to the on-chip detection and integration of the chip.
The disadvantages of this device are: the heating film, temperature sensor, and PCR microfluidic chip are integrated, the cost is high, and the bonding of the chip is difficult

Method used

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  • Multiple passage intelligent temperature-control device facing PCR microfluidic chip
  • Multiple passage intelligent temperature-control device facing PCR microfluidic chip
  • Multiple passage intelligent temperature-control device facing PCR microfluidic chip

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Embodiment Construction

[0026] Chip fixing device for a multi-channel intelligent temperature control device oriented to a PCR microfluidic chip In the present invention, a copper sheet with good thermal conductivity is used as a base material. The chip fixing device is insulated by the air belt between the heat insulation grooves with a width of 4mm and a length of 60mm. The present invention demonstrates the feasibility of the device from three aspects of theoretical calculation, numerical simulation and experimental detection.

[0027] Theoretical calculation: The heat transfer problem of the heat insulation groove is mainly divided into three parts: ①The heat radiation of the adjacent copper sheets of the heat insulation groove q = ϵσ ( T 100 ) 4 ; ②Insulation tank phase Radiative heat transfer between adjacent copper sheets q 1，2 =...

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Abstract

The invention discloses a multi-channel intelligent temperature-controlling device of polyase chain reaction micro-flow control chip, which comprises the following parts: three temperature sensors (3), three electric heat films (4), chip fixing device (1) and temperature collecting control device (2), wherein the chip fixing device is composed of three insulating areas (6), insulating groove (7) and pin on the insulating area with PCR micro-flow chip (5) on the surface of chip fixing device; the temperature collecting control device contains multi-path analog switch, A / D switcher, single chip set, D / A switcher, driving circuit and display.

Description

technical field [0001] A multi-channel intelligent temperature control device for polymerase chain reaction (PCR) microfluidic chips belongs to the field of micro-integrated automatic control for biochips. The device is mainly used in the independent setting and temperature control of the three constant temperature zones for DNA amplification on the PCR microfluidic chip. The whole system requires short heating time, small size, low cost and can communicate with PC. Background technique [0002] PCR microfluidic chip is a new technology that can rapidly and efficiently amplify DNA fragments of different lengths in vitro. The principle is to inject the PCR reaction mixture into the microchannels in three constant temperature zones on the substrate at a flow rate of nl / s under the action of the precision syringe pump, and realize the denaturation and renaturation of PCR by controlling the length of the microchannels. and extension. Its structure generally consists of an inle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/36C12P19/34
Inventor 左铁钏姚李英孔峥刘勋陈涛刘世炳
Owner BEIJING UNIV OF TECH
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