HLA-A resolution match chip preparation and uses

Abstract

The invention discloses a gene parting chip, which is characterized by the following: designing resolving typed probe in the atopic oligonucleotide according to gene point of common HLA-A of Chinese human populations according to real need; adopting primer marked by fluorescence; augumenting HLA-A on the multi-pattern area through fitful PCR method; crossing product and probe on the chip; scanning and analyzing the positive probe; affirming sample genetype; fitting for clinical and scientific study.

Description

Background technique [0001] HLA antigen is located in a narrow region of the short arm of human chromosome 6. It is the most complex genetic polymorphism system in the human body and plays an important role in the process of immune regulation. HLA polymorphism detection is of great significance in medical practice and scientific research. It provides reliable technical methods and valuable insights for the development of organ and tissue transplant matching, disease correlation research, anthropology and forensic applications. material. Serological method is the traditional classic typing method of HLA-B antigen, but with the in-depth research on HLA and the continuous improvement of typing technical requirements, and more and more alleles have been discovered and named one after another, serological The method has been unable to obtain enough monospecific antibodies, and cannot distinguish all the specificities, and the screening technology of standard antiserum is complicat...

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Problems solved by technology

Among them, PCR-SSP, PCR-SSO, and PCR-SSCP (single-strand conformation polymorphism analysis) three typing methods were all successful in typing antigens. But these methods also have some obvious deficiencies, such as PCR-SSP The method mainly relies on PCR technology, and a large number of primers are often designed when typing a certain gene individual, which will be difficult to avoid false positives caused by contamination problems in the subsequent PCR process, and the operation is complicated and needs to be passed Repeated electrophoresis to determine the final result is not suitable for typing large quantities of samples, which limits its clinical application; PCR-SSCP method is very effective for analyzing PCR amplification products less than 400bp, but this method can only detect genes The existence of variation, but the exact position and content of the variation cannot be determined, and it is generally only used for the analysis of the degree of HLA matching, not for the specific typing of HLA

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