Check patentability & draft patents in minutes with Patsnap Eureka AI!

UV quantitative measuring method for total phthalide compounds amount and application thereof

A quantitative determination and compound technology, applied in the field of ultraviolet quantitative determination, can solve the problems of high cost, high requirements for operators, unreasonable total amount of phthalide compounds, etc., and achieve the effect of reliable results and simple operation process

Inactive Publication Date: 2007-07-11
TIANJIN UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the determination results of the HPLC method and gas chromatography-mass spectrometry are relatively accurate, a reference substance with high purity and good stability of various components is required in the determination process, and quantitative determination must be carried out under a good separation. Therefore, the operating conditions are relatively strict. In addition, the equipment investment and daily analysis costs of these two methods are relatively large, and the requirements for operators are also relatively high, so industrial application is difficult.
In addition, these two methods can only measure the content of phthalide compounds with certain structures, but there are dozens of phthalide compounds with known structures at present, so the content of phthalide compounds with these structures is simply used to determine It is obviously unreasonable to characterize the total amount of phthalides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • UV quantitative measuring method for total phthalide compounds amount and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: UV quantitative determination method measures the total amount of phthalides in Ligusticum chuanxiong

[0025] (1) Accurately weigh 38.25 mg of sedanolactone with a purity of 98.10%, and dilute to 10 mL with absolute ethanol. Pipette 0, 250, 370, 500, 620, 750, 870 μL of the above solution, and dilute to 10 mL with absolute ethanol to obtain a series of reference substances with concentrations of 0, 93.80, 138.82, 187.60, 232.62, 281.40, 326.42 μg / mL standard solution.

[0026] (2) Accurately draw 1.0mL of the reference substance standard solution prepared in step (1) into a 10mL volumetric flask, add 4.0mL of 3% NaOH solution, then add absolute ethanol to dilute to the mark, shake well to make a sample, and simultaneously use Water and ethanol were used as reagent blank for control. Place the reagent blank and the sample in a water bath at 30°C for 30 minutes and keep them warm for 30 minutes. Using absolute ethanol as a reference, measure the absorbanc...

Embodiment 2

[0033] Embodiment 2: UV quantitative determination method is measured the total amount of phthalides in Angelica sinensis

[0034] (1) Accurately weigh 38.25 mg of sedanolactone with a purity of 98.10%, and dilute to 10 mL with absolute ethanol. Pipette 0, 250, 370, 500, 620, 750, 870 μL of the above solution, and dilute to 10 mL with absolute ethanol to obtain a series of reference substances with concentrations of 0, 93.80, 138.82, 187.60, 232.62, 281.40, 326.42 μg / mL standard solution.

[0035] (2) Accurately draw 1.0mL of the reference substance standard solution prepared in step (1) into a 10mL volumetric flask, add 4.0mL5% NaOH solution, then add absolute ethanol to dilute to the mark, shake well to make a sample, and simultaneously use Water and ethanol were used as reagent blank for control. Place the reagent blank and the sample in a water bath at 10°C for 60 minutes of insulated reaction. Using absolute ethanol as a reference, use a UV spectrophotometer to measure ...

Embodiment 3

[0042] Embodiment 3: UV quantitative determination method measures the total amount of phthalides in Ligusticum edulis

[0043] (1) Accurately weigh 38.25 mg of sedanolactone with a purity of 98.10%, and dilute to 10 mL with absolute ethanol. Pipette 0, 250, 370, 500, 620, 750, 870 μL of the above solution, and dilute to 10 mL with absolute ethanol to obtain a series of reference substances with concentrations of 0, 93.80, 138.82, 187.60, 232.62, 281.40, 326.42 μg / mL standard solution.

[0044] (2) Accurately draw 1.0mL of the reference substance standard solution prepared in step (1) into a 10mL volumetric flask, add 4.0mL of 3% NaOH solution, then add absolute ethanol to dilute to the mark, shake well to make a sample, and simultaneously use Water and ethanol were used as reagent blank for control. Place the reagent blank and the sample in a water bath at 30°C for 30 minutes and keep them warm for 30 minutes. Using absolute ethanol as a reference, measure the absorbance at...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention relates to the aromatic compounds ultraviolet quantitative measurement and application. It makes the aromatic compounds with known purity into serial of reference solution with density of 50-800 mug / mL by alcolate, adding strong queous alkali and alcohol, water bathing for 10-60 minutes in 10-60DEG C, measuring light absorbant at 290-340nm wavelength, with standard solution density as the horizontal ordinate, light absorbant as the vertical ordinate to draw standard curve, calculating the total content of aromatic compounds of the sample through standard curve. It s simple, quick and reliable free from particular instrument.

Description

technical field [0001] The invention relates to an ultraviolet quantitative determination method and application thereof, in particular to an ultraviolet quantitative determination method and application of the total amount of phthalide compounds. Background technique [0002] Phthalide compounds refer to a class of compounds with a phthalide core structure, which is one of the characteristic components of Umbelliferae plants, distributed in several families of Apiaceae, especially Amicel and Zephyr. The distribution is the most concentrated in the genus, mainly in plants such as Celery, Cnidium, Ligusticum, Angelica, Shanxiong and Euangia. In particular, the studied plants in the genus Ligusticum all contain phthalides, and more than 40 kinds of phthalides have been isolated and identified. It is known that phthalides are the dominant aroma components of their corresponding plants and have a wide range of biological activities. They are the main active ingredients of many ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/33
Inventor 杨学东孙广平丁明玉
Owner TIANJIN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com