Unlock instant, AI-driven research and patent intelligence for your innovation.

LANA viral protein binding sites

a technology of lana virus and binding site, which is applied in the field of lana virus protein binding site, can solve problems such as interfering with host processes, and achieve the effects of preventing transcription, strengthening interaction, and closer complementarity to perfection

Inactive Publication Date: 2004-12-09
RGT UNIV OF MICHIGAN
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

Enables the identification of compounds that inhibit or potentiate viral DNA binding, potentially leading to new therapeutic strategies for viral infections and tumors, and facilitates gene therapy by ensuring persistence of therapeutic genes in host cells.

Problems solved by technology

For example, the modified oligonucleotide (or "lof" oligonucleotide") may, in effect, function as a diminisher of natural gene function if the natural gene is present and functional in the host into which the lof oligonucleotide was transfected, or may negatively interfere with processes in the host if the natural gene is not present or is non-functional.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • LANA viral protein binding sites
  • LANA viral protein binding sites
  • LANA viral protein binding sites

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

Identification of LANA binding Sequences in Viral Genomes

[0158] A. Identification of Potential Consensus LANA-Binding Sites via Randomer Immunoprecipitation

[0159] In an effort to identify potential LANA-binding sites, a template for PCR amplification was synthesized which included KpnI and EcoRI restriction sites flanking a random 40-bp oligomer. Sense and antisense primers, which annealed to the nonrandom KpnI / EcoRI flanking sequences, were used to amplify a large pool of random 40-bp oligomers. This DNA was purified and mixed with anti-myc immunoprecipitates from 293 cells stably transduced with a myc-tagged LANA expression construct. After a period of 30 min, the immunoprecipitates were centrifuged out of the binding reaction and washed four times in binding buffer. These beads were then resuspended, heated at 95.degree. C. for 10 min, and then extracted with phenol:chloroform followed by chloroform isoamyl alcohol. Oligomers that had been precipitated with the LANA protein were ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
temperatureaaaaaaaaaa
Login to View More

Abstract

This invention relates to novel reagents and methods for the screening of compounds that may be agonistic or antagonistic to the binding of viral DNA binding proteins to viral DNA. The invention also relates to methods that may be used in gene therapy and that may be used to treat tumor viruses.

Description

[0002] This invention generally relates to novel compounds and methods for the detection of compounds that are agonistic or antagonistic for the binding of viral genetic material to genomic host DNA. Additionally, the inventions generally relates to compounds and methods related to gene transfer and gene therapy, as well as therapeutics for virally based diseases.[0003] In 1872, Moritz Kaposi described a multifocal vascular tumor affecting elderly men of Mediterranean or Eastern European origin. More recently, this neoplasm has become prevalent in immunocompromised patients, such as transplant recipients on immunosuppressive therapy, and AIDS patients, where it has become the most common cancer (Beral, V. "Epidemiology of Kaposi's sarcoma" Cancer Surv 10:5-22, 1991). The relationship of the disease to geography and immunocompromised patients led to the suspicion of an infectious agent in Kaposi's sarcoma pathogenesis. This suspicion was supported when KSHV or human herpesvirus 8 (HH...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C07K14/03C12N7/01C12Q1/70G01N33/569
CPCC07K14/005C12N2710/16222C12Q1/70G01N33/56983
Inventor ROBERTSON, ERLE S.
Owner RGT UNIV OF MICHIGAN