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Expression monitoring for human cytomegalovirus (HCMV) infection

a technology of human cytomegalovirus and expression monitoring, which is applied in the field of expression monitoring for human cytomegalovirus (hcmv) infection, can solve the problems of difficult to understand the functions and regulatory relationships of a large number of genes with traditional tools, and achieve the effect of preventing or ameliorating disease symptoms and increasing the probability of being infected

Inactive Publication Date: 2006-01-05
AFFYMETRIX INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach enables the identification of biologically relevant genes and their regulatory networks, providing insights into HCMV pathogenesis and disease progression, facilitating the identification of potential drug targets and understanding the host-pathogen interaction.

Problems solved by technology

Understanding the functions and regulatory relationships among the large number of genes is becoming a difficult task with traditional tools.

Method used

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  • Expression monitoring for human cytomegalovirus (HCMV) infection
  • Expression monitoring for human cytomegalovirus (HCMV) infection
  • Expression monitoring for human cytomegalovirus (HCMV) infection

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Summary

[0128] Mechanistic insights to viral replication and pathogenesis generally have come from the analysis of viral gene products, either by studying their biochemical activities and interactions individually or by creating mutant viruses and analyzing their phenotype. Now it is possible to identify and catalog the host cell genes whose mRNA levels change in response to the pathogen. We have employed DNA array technology to monitor the level of approximately 6600 human mRNAs in uninfected as compared to human cytomegalovirus-infected cells. The level of 258 mRNAs changed by a factor of 4 or more early after infection, before the onset of viral DNA replication. Several of these mRNAs encode gene products that might play key roles in virus-induced pathogenesis, identifying them as intriguing targets for further study.

Introduction

[0129] Human cytomegalovirus (HCMV) has the potential to alter cellular gene expression though multiple mechanisms. Its initial interaction with the ...

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Abstract

Certain human genes have been found to be induced or repressed in host cells infected with HCMV. A large set of such genes has been identified. These have diagnostic use in determining the extent of tissue damage caused by the infection as well as in determining the stage of disease progression of the HCMV infection. Such genes are likely those involved in mediating the pathology of the infected tissues. Thus by identifying agents which are able to reverse the induction or repression of such genes, one can find candidate therapeutic agents for use in treating and or preventing HCMV-caused disease pathologies.

Description

BACKGROUND OF THE INVENTION [0001] Many biological functions are accomplished by altering the expression of various genes through transcriptional (e.g. through control of initiation, provision of RNA precursors, RNA processing, etc.) and / or translational control. For example, fundamental biological processes such as cell cycle, cell differentiation and cell death, are often characterized by the variations in the expression levels of groups of genes. [0002] Gene expression is also associated with pathogenesis. For example, the lack of sufficient expression of functional tumor suppressor genes and / or the over expression of oncogene / protooncogenes could lead to tumorigenesis (Marshall, Cell, 64:313-326 (1991); Weinberg, Science, 254: 1138-1146 (1991), incorporated herein by reference for all purposes). Thus, changes in the expression levels of particular genes (e.g. oncogenes or tumor suppressors) serve as signposts for the presence and progression of various diseases. [0003] The study...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/70C12Q1/68C12Q1/6837C12Q1/6883
CPCC12Q1/6837C12Q1/6883G01N2333/045C12Q2600/158C12Q1/705
Inventor ZHU, HUAGINGERAS, THOMASSHENK, THOMAS
Owner AFFYMETRIX INC