Composition of restricted cancer cells which produce cancer cell proliferation suppressive materials, and uses thereof

a cancer cell and suppressive material technology, applied in the direction of tumor/cancer cells, unknown materials, antibody medical ingredients, etc., can solve the problems of host fibrotic response to implant material, limited success, instability of implant material,

Inactive Publication Date: 2007-05-17
ASINA SHIRIN +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The encapsulation of various biological materials in biologically compatible materials, which is well documented in the literature, is a technique that has been used for some time, albeit with limited success.
There are many problems associated with methods of the prior art, including a host fibrotic response to the implant material, instability of the implant material, limited nutrient diffusion across semi-permeable membranes, secretagogue and product permeability, and diffusion lag-time across semi-permeable membrane barriers.
Other investigators, however, repeating these experiments, found the alginate to cause a tissue reaction and were unable to reproduce Lim, et al.
Their method, however, suffers from a number of drawbacks.
It is cumbersome and inaccurate.
Furthermore, the transplanted beads are difficult to retrieve, tend to be fragile, and will easily release islets upon slight damage.
They found that when the islets are injected into the fiber, they aggregate within the hollow tube with resultant necrosis in the central portion of the islet masses.
However, this experiment has not been repeated extensively.
Therefore, the membrane's function as an islet transplantation medium in humans is questionable.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] This example, and those which follow, employ RENCA cells. These are spontaneous renal adenocarcinoma cells of BALB / C mice, which are widely available, having been maintained in both in vitro cultures and in vivo. See Franco, et al., Cytokine Induced Tumor Immunogenecity, 181-193 (1994).

[0024] Samples of frozen RENCA cells were thawed at 37° C., and then placed in tissue culture flasks containing Dulbecco's Modified Medium (D-MEM), which had been supplemented with 10% bovine serum, penicillin (100 u / ml) and streptomycin (50 ug / ml), to give what will be referred to as “complete medium” hereafter.

[0025] Cells were grown to confluence, and then trypsinized, followed by washing with Hank's Balanced Salt Solution, and then with the complete medium referred to supra.

[0026] In order to determine if the RENCA cells produced tumors efficiently, two BALB / C mice were injected, intraperitoneally, with 106 of these cells. The mice were observed, over a 3-4 week period. Clinically, they ...

example 2

[0028] Following the showing that the RENCA cells did grow in vivo, studies were carried out to determine if these cells grew when restricted in the structure of the invention.

[0029] RENCA cells were grown to confluency, as described supra, trypsinized, and washed, also as described above. Samples of between 60,000 and 90,000 cells were then prepared. The cells were then centrifuged, at 750 RPMs, and fluid was removed. The cells were then suspended in solutions of 1% atelocollagen, in phosphate buffered saline solution, at a pH of 6.5.

[0030] A 1% solution of low viscosity agarose was prepared in minimal essential medium (MEM), maintained at 60° C., and then 100 ul of this was added to the suspension of RENCA cells and atelocollagen, described supra. The materials were then transferred, immediately, as a single large droplet, into sterile, room-temperature mineral oil. The mixture formed a single, smooth, semi-solid bead. This procedure was repeated to produce a number of beads.

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example 3

[0033] Prior to carrying out in vivo investigations, it was necessary to determine if the RENCA cells would grow in beads prepared in the manner described supra.

[0034] To do this, beads prepared as discussed in example 2 were incubated in the medium described in example 2, for a period of three weeks, under the described conditions. Three of the beads were then cut into small pieces, and cultured in standard culture flasks, affording direct contact with both the flask and culture medium.

[0035] Observation of these cultures indicated that the cells grew and formed standard RENCA colonies. This indicated that the cells had remained viable in the beads.

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Abstract

Compositions of matter are described which contain restricted cancer cells. When so restricted, the cells produce an unexpectedly high amount of material which suppresses cancer cell proliferation. The phenomenon crosses cancer type and species lines. Processes for making these compositions, and their use, are also described.

Description

RELATED APPLICATION [0001] This application is a continuation in part of allowed patent application Ser. No. 08 / 745,063, filled on Nov. 7, 1996, which is a continuation-in-part of co-pending application Ser. No. 08 / 625,595, filed Apr. 3, 1996 now abandoned. Both are incorporated by reference.FIELD OF THE INVENTION [0002] The present invention relates to the restriction of the proliferation of cancer cells to produce material which suppresses proliferation of unrestricted cancer cells. The structures which are one feature of the invention can be used “as is,” or to produce material such as concentrates with a minimum approximate molecular weight, which also have an anti-proliferative effect on cancer. BACKGROUND AND PRIOR ART [0003] The encapsulation of various biological materials in biologically compatible materials, which is well documented in the literature, is a technique that has been used for some time, albeit with limited success. Exemplary of the art are U.S. Pat. Nos. 5,227...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/00A61K35/12A61K35/22A61K35/23C12N5/09C12N11/04
CPCA61K35/22A61K39/0011A61K2035/126A61K2035/128C12N5/0693C12N11/04
Inventor ASINA, SHIRINJAIN, KANTIRUBIN, ALBERT L.SMITH, BARRYSTENZEL, KURT
Owner ASINA SHIRIN
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