Hyaluronan synthase genes and expression thereof in Bacillus hosts
a technology of hyaluronan synthase and host, applied in the field of nucleic acid segment, can solve the problems of insufficient development of vitro systems, inability to fully understand the mechanism of ha synthesis, and inability to produce at least one, so as to improve translational efficiency, increase the half-life, and improve the effect of production
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[0070] Before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not limited in its application to the details of construction and the arrangement of the components set forth in the following description or illustrated in the drawings. The invention is capable of other embodiments or of being practiced or carried out in various ways. Also, it is to be understood that the phraseology and terminology employed herein is for purpose of description and should not be regarded as limiting.
[0071] As used herein, the term “nucleic acid segment” and “DNA segment” are used interchangeably and refer to a DNA molecule which has been isolated free of total genomic DNA of a particular species. Therefore, a “purified” DNA or nucleic acid segment as used herein, refers to a DNA segment which contains a Hyaluronate Synthase (“HAS”) coding sequence yet is isolated away from, or purified free from, unrelated genomic DNA of the source cell. Inclu...
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