Cysteine granules and use thereof as bifidobacterium animalis lactis growth stimulants

a technology of bifidobacterium animalis and cysteine granules, which is applied in the field of cysteine granules and use thereof as bifidobacterium animalis lactis growth stimulants, can solve the problems of difficult to achieve good compromise between the growth of technological ferments and probiotics, the quantity of activator compounds which have to be used in order to provide stimulation and the above constraints are generally very small, and the problem of difficult to

Inactive Publication Date: 2009-12-31
DANONE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0020]Despite the low solubility of the particular L-cysteine selected by the inventors, the inventors have succeeded in producing an inoculum in the form of frozen granules and / or lyophilisate(s) which combine or integrate cells of B. animalis lactis and this particular form of L-cysteine, without in any way damaging the physiological condition and / or the metabolism of B. animalis lactis.

Problems solved by technology

A good compromise between the growth of technological ferments and probiotics is difficult to achieve; the majority of industry professionals favor the organoleptic aspect over the quantity of probiotic: generally, the probiotic population is between 106 and 107 cfu / g of fermented product.
Furthermore, the quantities of activator compounds which have to be used in order to provide stimulation and which satisfy the above constraints are generally very small as a proportion with respect to the dairy substrates.
Such doses (generally less than 100 mg / L) and the allowable tolerances are not compatible with the measurement systems and the modes of incorporation used to produce the dairy substrates, and so using such substances on an industrial scale is problematic.
The problem is further complicated when the technical strains employed have to function in the context of the symbiosis of several strains of lactic bacteria, as is the case with fermented milks which use a symbiotic fermentation of S. thermophilus and L bulgaricus in addition to the lactic fermentation occurring with the probiotics.

Method used

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  • Cysteine granules and use thereof as bifidobacterium animalis lactis growth stimulants
  • Cysteine granules and use thereof as bifidobacterium animalis lactis growth stimulants
  • Cysteine granules and use thereof as bifidobacterium animalis lactis growth stimulants

Examples

Experimental program
Comparison scheme
Effect test

example 1

Using Cysteine-HCl is not Suitable for the Manufacture of Granules Termed “Integrated” Granules which would Comprise both Cysteine and B. animalis lactis

Process and Apparatus

[0314]In this example, the granule manufacturing principle shown in FIG. 2 was followed.

[0315]The following probiotic strains were used in this example:[0316]Bifidobacterium animalis lactis strain BB12® (sold by Chr Hansen A / S, Hoersholm, Denmark;[0317]Bifidobacterium animalis lactis strain I-2494 available from the CNCM (date of deposit with CNCM=20 Jun. 2000); see International patent application WO-02 / 02800 filed on 4 Jul. 2001 in the name of Compagnie Gervais Danone and the national and regional counterparts of this international application; more particularly as regards the US counterparts of this international application, see U.S. Pat. No. 7,008,785.

[0318]If necessary, the strains were defrosted before use.

[0319]The cysteine used was cysteine-HCl, which is normally used to provide cysteine because of its...

example 2

Selection of an L-Cysteine Base which is Suitable for the Production of Highly Effective “Integrated” Granules Despite Problems Linked to Low Solubility of this Form of Cysteine

[0353]The procedure was comparable to that described in Example 1 above, but L-cysteine base (L-cysteine; C3H7NO2S; CAS number: 52-90-4; molar mass: 121.16) was selected instead of and in place of the cysteine-HCl.

[0354]L-cysteine base is available from Ajinomoto Aminoscience LLC, Raleigh N.C. 27610, USA.

[0355]The solubility of said cysteine base is 16 g per 100 g of H2O at 20° C.

[0356]The pH of a solution containing 1.15 g of cysteine base per 50 mL of H2O is 4.5 to 5.5.

[0357]A stock solution was prepared containing 160 g / L of cysteine base and 3 grams of this stock solution was mixed with 10 grams of B. animalis lactis ferment (B. animalis lactis strain I-2494 or strain BB12®) which was formulated into frozen granules (I-2494 G4I Cys-base; and BB12 G4I Cys-base) as described in Example 1 above.

[0358]Referen...

example 3

Performance of Integrated G4I Cys-Base System Firstly Compared with the Combined G4 System (Cysteine Granules Distinct from Probiotic Ferment Granules) and Secondly Compared with a Reference System (T Granules, No Cysteine)

[0369]In this example, granules of B. animalis lactis ferment produced as described in Example 2 were used, namely:[0370]BB12 T granules (reference granules containing B. animalis lactis BB12®+water);[0371]BB12 G4I granules (integrated granules containing B. animalis lactis BB12®+L-cysteine base in a concentration of approximately 1.85 mg of L-cysteine, C3H7NO2S, per billion cfu of probiotic ferment);[0372]I-2494 T granules (reference granules containing B. animalis lactis I-2494+water); and[0373]I-2494 G4I granules (integrated granules containing B. animalis lactis I-2494+L-cysteine base in an amount of approximately 1.85 mg of cysteine, C3H7NO2S, per billion cfu of probiotic ferment).

[0374]In this example, ferments termed technical ferments were also used; they ...

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Abstract

The present application relates to an inoculum that is particularly suitable for the direct inoculation of at least one strain of Bifidobacterium animalis ssp. lactis in a dairy substrate, for the conversion of said dairy substrate into a fermented dairy product. This inoculum comprises, in mixture or in collection form, the L-cysteine base and at least one strain of B. animalis lactis. Said cysteine and said at least one strain of B. animalis lactis are each contained in, or in the form of frozen and / or lyophilizate granules. Advantageously, said cysteine is incorporated within the same granules or the same lyophilizate as the cells of B. animalis lactis.

Description

FIELD OF THE INVENTION[0001]The present invention relates to granules of cysteine and to their uses as growth stimulants for Bifidobacterium animalis ssp lactis. PRIOR ART OF THE INVENTION[0002]In the dairy products field, the probiotics market is burgeoning.[0003]In order to propose products containing high levels of probiotic strains while providing advantageous organoleptic qualities, dairy professionals are confronted with problems with mixed fermentations which employ both technical or technological ferments as well as probiotic ferments.[0004]Strains termed probiotic strains are represented by species which grow slowly on a dairy substrate (generation time 1 h to 2 h for Bifidobacterium), while technological ferments derive from yoghurt ferments which grow rather more rapidly (generation time 15 min to 30 min for S. thermophilus and L. bulgaricus).[0005]A good compromise between the growth of technological ferments and probiotics is difficult to achieve; the majority of indust...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A23C9/12
CPCA23C9/1234A23C9/1322A23V2002/00A23V2250/0616A23V2200/08
Inventor MARCHAL, LAURENTDHOOSCHE, XAVIER
Owner DANONE
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