New competence stimulating peptide

Inactive Publication Date: 2012-02-16
INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
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  • Abstract
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Benefits of technology

[0017]The present invention also relates to a culture medium comprising an effective amount of said isolated polypeptide and nutrients that allow the growing of Firmicutes, in particular Streptococcus, and more preferably S. thermophilus.
[0018]The present invention further relates to a use of said polypeptide, said nucleic acid, said vector, said host cell, or said culture medium for stimulating competence in Firmicutes, in particular Streptococcus, and more preferably S. thermophilus.
[0019]The present

Problems solved by technology

The sensing function is more complex and is poorly documented, particularly in nonpathogenic bacteria.
However, until now, only genetic tools based on genetically modified bacteria exist.

Method used

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examples

1) The Anti Transporter Controls the Synthesis of Several Proteins Essential for Natural Transformation in Streptococci

[0083]In order to find physiological functions controlled by signaling peptides that are internalized by the Ami transporter, we compared the proteome of the wild type LMD-9 strain and its isogenic mutant deleted for the ami operon, LMD-9 ΔamiCDE (TIL883; IBRAHIM et al., J. Bacteriol., vol. 189, p: 8844-8854, 2007).

[0084]In order to by-pass the nutritional function of the Ami transporter, cells were gown in CDM, a free-peptide chemically defined medium (CDM), containing only amino acids as nitrogen source, as described by Letort & Juillard, Development of a minimal chemically-defined medium for the exponential growth of Streptococcus thermophilus, J. Appl. Microbiol., vol. 91, p. 1023-1029, 2001. Optical density at 600 nm (OD600) of the cultures was measured using a spectrophotometer UVIKON 931 (KONTRON).

[0085]Proteins were prepared from cells grown in CDM and harve...

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Abstract

The present invention concerns a new competence stimulating peptide identified in Firmicutes, in particular Streptococcus, and more preferably S. thermophilus and methods of producing transformation competent Firmicutes, in particular Streptococcus, and more preferably S. thermophilus bacteria.

Description

DOMAIN OF THE INVENTION[0001]The present invention relates to Firmicutes, in particular Streptococcus, and more preferably S. thermophilus competence, and more specifically to a new competence stimulating peptide.BACKGROUND OF THE INVENTION[0002]Many species of bacteria control gene expression on a community-wide scale by producing, secreting, detecting and responding to extracellular signaling molecules (sometimes called ‘autoinducers’ or ‘pheromones’) that accumulate in the environment. This phenomenon is termed ‘quorum sensing’ (QS) as gene expression is triggered by the ‘sensing’ of the pheromone when its concentration has reached a ‘quorum’. In Gram-positive bacteria, the signaling molecules are mainly short peptides acting either from the outside part of bacteria or from the inside, after internalization via oligopeptide transport systems called Opp or Ami.[0003]Several bacterial functions such as the virulence in Staphylococcus aureus and Enterococcus faecalis, the competence...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C07K14/315C12N15/01C12N15/63C12N1/21C07K7/06C12N15/31
CPCC07K14/315C12N15/746C12N1/20C12N1/38
Inventor GARDAN, ROZENNMONNET, VERONIQUE
Owner INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
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