Western blot kit for detection of vaccinated poultry

Abstract

Modified western blot membranes with silver nanoparticle allow the small peptides of the NS1 protein of the poultry influenza virus to be kept in the membrane and not to diffuse during transferring from the Tricine SDS PAGE. These peptides may differentiate infected from vaccinated poultry.

Description

FIELD OF THE INVENTION[0001]This kit could differentiate infected poultry from vaccinated during influenza outbreak.BACKGROUND OF THE INVENTION[0002]Differentiating infected from vaccinated animals is known as DIVA strategy. Vaccination is primarily performed using killed whole virus-adjuvanted vaccines. Proper vaccination may reduce or prevent clinical signs, reduce virus shedding in infected birds, and increase the resistance to infection.[0003]One limitation of killed whole-virus vaccines is that vaccinated birds cannot be distinguished serologically from naturally infected birds using commonly available diagnostic tests. Thus detection of avian influenza becomes much more difficult and often results in trade restrictions because of the inability in DIVA strategy to distinguish between vaccinated and infected individuals.[0004]The most common method to distinguish vaccinated individuals from infected is the use of unvaccinated sentinels. A second approach is the use of subunit va...

AI Technical Summary

Benefits of technology

The patent text describes a kit that can distinguish infected poultry from vaccinated poultry during avian influenza outbreaks. The kit uses a conserved peptide from the NS1 protein of the virus to detect antibodies in infected poultry. The kit can be used in any context where it is necessary to differentiate between vaccinated and infected individuals, such as humans, cattle, poultry, and pork. The kit can be used to discriminate between infected and vaccinated individuals during avian influenza outbreaks. The kit can also be used to differentiate between infected and vaccinated individuals caused by other infectious diseases.

Problems solved by technology

One limitation of killed whole-virus vaccines is that vaccinated birds cannot be distinguished serologically from naturally infected birds using commonly available diagnostic tests.

Thus detection of avian influenza becomes much more difficult and often results in trade restrictions because of the inability in DIVA strategy to distinguish between vaccinated and infected individuals.

However, poultry vaccines are not highly purified, and they contain small amounts of the NS1 protein.

They developed an ELISA system with these peptides to differentiate the infected and vaccinated poultry, but because of the small size of the peptides, it was not possible to develop a western blot method.