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Conversion of non-neuronal cells into neurons

a technology of non-neuronal cells and neurons, applied in the direction of genetically modified cells, skeletal/connective tissue cells, instruments, etc., can solve the problems of shortened neuron development time course, 19 days post antibiotic selection, and expensive neurotrophic factors

Inactive Publication Date: 2017-01-05
UNIV OF SOUTH FLORIDA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This approach reduces the conversion time to 6-8 days and eliminates the requirement for costly neurotrophic factors, enabling faster and more cost-effective production of neuronal cells for drug screening and therapeutic evaluation in Alzheimer's disease and other neurodegenerative conditions.

Problems solved by technology

This method has shortened the time course of neuron development, but it still takes 19 days post antibiotic selection and requires expensive neurotrophic factors.

Method used

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  • Conversion of non-neuronal cells into neurons
  • Conversion of non-neuronal cells into neurons
  • Conversion of non-neuronal cells into neurons

Examples

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example 1

[0040]Fibroblasts obtained from human skin are cultured on a coverslip coated with poly-D-lysine and laminin overnight. The following day, cells are transduced with lentivirus containing PTB1 shRNA for 16 hours and the medium is replaced. After 24 hours, the cells are selected using hygromycin (100 ng / ml) for 48 hours. The neural induction medium containing (10 μM) Rock inhibitor is added and cells are incubated for 6-8 days for induced neuronal development (see FIGS. 1-4). As shown in FIG. 5, the induced neurons are able to uptake monomeric Tau and form Tau aggregates inside the cell. In summary, we have successfully induced neurons from human skin fibroblast in half the time that is reported by others and without using any expensive neurotrophic factors. This newly discovered method will hasten personalized medicine approaches for Alzheimer's disease and possibly many other neurodegenerative diseases.

[0041]It should be understood that the examples and embodiments described herein ...

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Abstract

The subject invention concerns materials and methods for conversion of non-neuronal cells into neurons. The subject invention also concerns methods for screening drugs and other compounds for activity in treating Alzheimer's disease using neuronal cells produced using the subject invention. The subject invention also concerns neuronal cells that have been produced using the methods of the invention. The subject invention also concerns methods for evaluating therapeutic treatment for efficacy in a person or animal having Alzheimer's disease or other neurodegenerative diseases. The subject invention also concerns methods of treating Alzheimer's disease or other neurodegenerative diseases or conditions in a person or animal.

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This application is a divisional of U.S. application Ser. No. 14 / 788,045, filed Jun. 30, 2015, which is hereby incorporated by reference herein in its entirety, including any figures, tables, nucleic acid sequences, amino acid sequences, or drawings.BACKGROUND OF THE INVENTION[0002]Alzheimer's disease (AD) is most common cause of dementia amongst people over 65 years old (National Institute of Aging). Increasing evidence suggests that multi-factorial events occur between the initial phases of the disease and the end stage pathology. One of the most common proteins in AD pathology is a Tau, a soluble, microtubule-associated protein known to aberrantly form amyloid-positive aggregates. Therefore, developing an ex vivo assay to screen drugs using individual patient cells to slow or diminish these aggregates would be helpful in discovering new therapies for AD. Recent advances in stem cell research have rekindled newer ways of synthesizing the...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/0793C12N15/86G01N33/50A61K35/30
CPCA61K35/30A61P25/28C12N5/0619C12N15/111C12N15/113C12N15/1138C12N2310/113C12N2310/122C12N2310/531C12N2320/30C12N2501/727C12N2506/1307C12N2510/00C12N15/86C12N2500/32C12N2500/34C12N2501/734C12N2501/998C12N2501/999C12N2506/45C12N2740/15043G01N33/5058
Inventor KAMATH, SIDDHARTH G.DICKEY, CHADCHERPELIS, BASIL
Owner UNIV OF SOUTH FLORIDA
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